| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Research Abstract |
In the present study, we analyzed an enhancer sequence composed of Malacosma Neustria nucleopolyhedrovirus polyhedorin 5'-UTR for improvement of insect cell-free protein synthesis system. As a result, we found important sequence of 16 base length. A protein synthesis activity using twice repeat of the sequence as enhancer was 1.4-fold greater than that of using usual enhancer. Furthermore, we constructed cell-free expression and analysis systems for organic solvent resistant tyrosinase(metal protein) and thermostable laccase(membrane-binding protein)
|
