An improvement of insect cell-free protein synthesis system
| Project/Area Number |
21580069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Okinawa National College of Technology |
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Principal Investigator |
ITO Masaaki 沖縄工業高等専門学校, 生物資源工学科, 教授 (00395659)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 昆虫利用・機能開発 / 無細胞タンパク質合成系 / 昆虫 / 昆虫培養細胞 / 無細胞タンパク質合成 / 無細胞翻訳系 / 翻訳促進配列 / カイコ |
|---|
| Research Abstract |
In the present study, we analyzed an enhancer sequence composed of Malacosma Neustria nucleopolyhedrovirus polyhedorin 5'-UTR for improvement of insect cell-free protein synthesis system. As a result, we found important sequence of 16 base length. A protein synthesis activity using twice repeat of the sequence as enhancer was 1.4-fold greater than that of using usual enhancer. Furthermore, we constructed cell-free expression and analysis systems for organic solvent resistant tyrosinase(metal protein) and thermostable laccase(membrane-binding protein)
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Development of an insect cell-free system2010
Author(s)
Suzuki, T., Ezure, T., Shikata, M., Ito, M., Ando, E., Utsumi, T., Nishimura, O., and Tsunasawa, S
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Journal Title
Current Pharmaceutical Biotechnology
Volume: 11
Pages: 279-284
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[Journal Article]2009
Author(s)
伊東昌章
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Journal Title
ラッカーゼ-その特性と環境分野への利用-「産業酵素の応用技術と最新動向」(井上國世監修)(シーエムシー出版)
Pages: 321-328
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[Journal Article]2009
Author(s)
Suzuki, T., Ezure, T., Ito, M., Shikata, M., Ando, T.
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Journal Title
An insect cell-free system for recombinant protein expression using cDNA resources in Reverse Chemical Genetics, Methods in Molecular Biology, vol. 577(Humana Press)
Pages: 97-108
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[Book] A cell-free protein synthesis system from insect cells in Cell-free Protein Production, Methods in Molecular Biology2010
Author(s)
Ezure, T., Suzuki, T., Shikata, M., Ito, M., and Ando, E.
Publisher
Humana Press
Related Report
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[Book] "A cell-free protein synthesis system from insect cells" in Cell-free Protein Production, Methods in Molecular Biology, vol.6072010
Author(s)
Ezure, T., Suzuki, T., Shikata, M., Ito, M., Ando, E.
Publisher
Humana Press
Related Report
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[Book] An insect cell-free system for recombinant protein expression using cDNA resources in Reverse Chemical Genetics, Methods in Molecular Biology2009
Author(s)
Suzuki, T., Ezure, T., Ito, M., Shikata, M., and Ando, T.
Publisher
Humana Press
Related Report
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