| Project/Area Number |
21580070
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
KIUCHI Makoto 独立行政法人農業生物資源研究所, 昆虫科学研究領域, 領域長 (90391549)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIMURA Manabu (独)農業生物資源研究所, 昆虫成長制御研究ユニット, 主任研究員 (60370649)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 昆虫生理 / 休眠 / 脱皮ホルモン / 緑きょう病菌 / オオワタノメイガ / 脱皮ホルモン分解酵素 |
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| Research Abstract |
Many researches suggested that larval and pupal diapauses are maintained by ecdysteroid deficiency, however, the endocrinological cue to trigger diapause is still not very clear. We therefore examined the role of ecdysteroid for the induction of the larval diapause of Haritalodes basipunctalis. When non-diapausing larvae of H. basipunctalis were injected with an ecdysteroid inactivating enzyme ecdysteroid-22-oxidase(E22O) identified from Nomuraea rileyi, they survived for a few months without eating anything. The E22O-injected larvae also acquired high cold-hardiness, a characteristic often observed in insects in diapause. Furthermore, they resumed development after being exposed to chilling conditions, which is the environmental stimulus to break diapause in many insect species. These developmental and survival characteristics were indistinguishable from those of the normal diapausing larvae, suggesting strongly that the E220 injection induced the larval diapause in H. basipunctalis. To the best of our knowledge, this is the first report indicating that a reduction in the ecdysteroid titer is a sufficient endocrinological stimulus to induce diapauses in insects.
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