Functional analysis of genes from an anhydrobiotic chironomid, Polypedilum vanderplanki, using the transgenic fly system

• Project/Area Number: 21580071
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: KANAMORI Kanamori yasushi 京都工芸繊維大学, ショウジョウバエ遺伝資源センター, 研究員 (70535799)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Masatoshi 京都工芸繊維大学, ショウジョウバエ遺伝資源センター, 教授 (10142001) ; KIKAWADA Takahiro 農業生物資源研究所 (60414900) ; OKUDA Takashi 農業生物資源研究所 (60414893)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 乾燥耐性 / ネムリユスリカ / トレハロース / LEAタンパク質 / キイロショウジョウバエ / ストレス / 糖

Research Abstract

To analysis the functions of trehalose and four LEA proteins in the dried state of an anhydrobiotic chironomid, Polypedilum vanderplanki larva, desiccation-related genes were introduced into Drosophila melanogaster. The adult flies expressing four LEA protein genes had no additional tolerance for desiccation ; moreover, PvLEA1-expressed male fly presented the sensitivity for desiccation. This suggests that PvLEA proteins concern with desiccation tolerance of P. vanderplanki indirectly.

Research Products

• [Journal Article] Identification of anhydrobiosis-related genes from an expressed sequence tag database in the crypto biotic midge Polypedilum vanderplanki(Diptera ; Chironomidae). (2010)
  • Author(s): Cornette, R., Kanamori, Y., Watanabe, M., Nakahara, Y., Gusev, O., Mitsumasu, K., Kadono-Okuda, K., Shimomura, M., Mita, K., Kikawada, T, Okuda, T
  • Journal Title: J Biol Chem Volume: Vol.285 Pages: 35889-35899
  • Peer Reviewed
• [Journal Article] Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki. (2010)
  • Author(s): Mitsumasu, K., Kanamori, Y., Fujita, M., Iwata, K., Tanaka, D., Kikuta, S., Watanabe, M., Cornette, R., Okuda, T., Kikawada, T.
  • Journal Title: FEBS J. Volume: Vol.27 Pages: 4215-4228
  • Peer Reviewed
• [Journal Article] Desiccation-induced structuralization and glass formation of group 3 late embryogenesis abundant protein model peptides (2010)
  • Author(s): Shimizu, T., Kanamori, Y., Furuki, T., Kikawada, T., Okuda, T., Takahashi, T., Mihara, H., Sakurai, M.
  • Journal Title: Biochemistry Volume: Vol.40(6) Pages: 1093-1104
  • Peer Reviewed
• [Journal Article] The trehalose transporter 1 gene sequence is conserved in insects and encodes proteins with different kinetic properties involved in trehalose import into peripheral tissues (2010)
  • Author(s): Kanamori, Y., Saito, A., Hagiwara-Komoda, Y., Tanaka, D., Mitsumasu, K., Kikuta, S., Watanabe, M., Cornette, R., Kikawada, T., Okuda, T.
  • Journal Title: Insect Biochem Mol Biol Volume: Vol.40 Pages: 30-37
  • Peer Reviewed
• [Journal Article] Identification of anhydrobiosis-related genes from an expressed sequence tag database in the cryptobiotic midge Polypedilum vanderplanki (Diptera ; Chironomidae) (2010)
  • Author(s): Cornette R, Kanamori Y, Watanabe M, Nakahara Y, Gusev O, et al
  • Journal Title: J Biol Chem Volume: 285 Pages: 35889-35899
  • Peer Reviewed
• [Journal Article] A Eukaryotic (Insect) Tricistronic mRNA Encodes Three Proteins Selected by Context-dependent Scanning (2010)
  • Author(s): Kanamori Y, Hayakawa Y, Matsumoto H, Yasukochi Y, Shimura S, et al
  • Journal Title: J Biol Chem Volume: 285 Pages: 36933-36944
  • Peer Reviewed
• [Journal Article] Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki (2010)
  • Author(s): Mitsumasu K, Kanamori Y, Fujita M, Iwata K, Tanaka D, et al
  • Journal Title: FEBS J Volume: 277 Pages: 4215-4228
  • Peer Reviewed
• [Journal Article] The trehalose transporter 1 gene sequence is conserved in insects and encodes proteins with different kinetic properties involved in trehalose import into peripheral tissues (2010)
  • Author(s): Kanamori, Y., Saito, A., Hagiwara-Komoda, Y., Tanaka, D., Mitsumasu, K., Kikuta, S., Watanabe, M., Cornette, R, Kikawada, T., Okuda, T.
  • Journal Title: Insect Biochem Mol Biol Vol. 40 Pages: 30-37
  • Peer Reviewed
• [Journal Article] Desiccation-induced structuralization and glass formation of group 3 late embryo genesis abundant protein model peptides (2010)
  • Author(s): Shimizu, T., Kanamori, Y., Furuki, T., Kikawada, T., Okuda, T., Takahashi, T., Mihara, H., Sakurai, M.
  • Journal Title: Biochemistry Vol. 49(6) Pages: 1093-1104
  • Peer Reviewed
• [Presentation] ショウジョウバエにおけるトレハローストランスポーター遺伝子の重複と進化 (2012)
  • Author(s): 金森保志
  • Organizer: 平成24年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2012-03-19
• [Presentation] ショウジョウバエにおけるトレハローストランスポーター遺伝子の重複と進化 (2012)
  • Author(s): 金森保志
  • Organizer: 日本蚕糸学会「平成24年度蚕糸・昆虫機能利用学術講演会」
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2012-03-19
• [Presentation] ネムリユスリカの乾燥耐性関連遺伝子を導入したキイロショウジョウバエにおける耐性能力検証の試み (2011)
  • Author(s): 金森保志
  • Organizer: 第12回極限環境生物学会
  • Place of Presentation: 長崎大学(長崎県)
  • Year and Date: 2011-11-28
• [Presentation] 遺伝子組換えショウジョウバエによるネムリユスリカ乾燥耐性関連遺伝子の研究 (2011)
  • Author(s): 金森保志
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2011-09-22
• [Presentation] 遺伝子組換えショウジョウバエによるネムリユスリカ乾燥耐性関連遺伝子のin vivo解析 (2011)
  • Author(s): 金森保志
  • Organizer: 平成23年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 要旨掲載による発表
  • Year and Date: 2011-03-20
• [Presentation] 遺伝子組換えショウジョウバエによるネムリュスリカ乾燥耐性関連遺伝子のin vivo解析 (2011)
  • Author(s): 金森保志
  • Organizer: 平成23年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: (要旨掲載による発表)
  • Year and Date: 2011-03-20
• [Presentation] ネムリユスリカのゲノム構造から見えてきた乾燥耐性獲得の進化過程 (2010)
  • Author(s): 金森保志
  • Organizer: 第54回日本応用動物昆虫学会小集会
  • Place of Presentation: 千葉大学(千葉県)
  • Year and Date: 2010-03-27
• [Presentation] ネムリユスリカのゲノム構造から見えてきた乾燥耐生獲得の進化過程 (2010)
  • Author(s): 金森保志
  • Organizer: 第54回日本応用動物昆虫学会小集会
  • Place of Presentation: 千葉大学, 西千葉キャンパス (千葉県)
  • Year and Date: 2010-03-27
• [Presentation] Functional analysis of genes from an anhydrobiotic chironomid, Polypedilum vanderplanki, using the transgenic fly system. (2010)
  • Author(s): Kanamori Y
  • Organizer: International Bioresource Symposium "Drosophila"
  • Place of Presentation: 延暦寺会館(滋賀県)
  • Year and Date: 2010-03-17
• [Presentation] Functional analysis of genes from an anhydrobiotic chironomid, Polypedilum vanderplanki, using the transgenic fly system (2010)
  • Author(s): Kanamori Y, Gusev O, Mitsumasu K, Cornette R, Kikawada T, Watanabe M, Okuda T, Takahashi A, Yamada H, Yamamoto MT
  • Organizer: International Bioresource Symposium "Drosophila"
  • Place of Presentation: 延暦寺会館 (滋賀県)
  • Year and Date: 2010-03-17