Functional characterization of two-component high-affinity nitrate transport system

• Project/Area Number: 21580076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Niigata University
• Principal Investigator: SUEYOSHI Kuni 新潟大学, 自然科学系, 准教授 (10216278)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: オオムギ / 硝酸イオン / 膜輸送 / トランスポーター / 輸送 / 組織内局在性

Research Abstract

In barley, low level of external nitrate is absorbed by high affinity transport system(HATS) which is composed of two proteins, HvNRT2 and HvNAR2. In this study, it was suggested that two proteins make complex in the isolated plasma membrane and co-localized at out most layer of the cells in root cortex. In addition, Bimolecular fluorescence complementation analysis showed that HvNRT2 and HvNAR2 interacts in the plasma membrane of the native plant cells.

Research Products

• [Journal Article] Two-component high-affinity nitrate transport system in barley : membrane localization, protein expression in roots and a direct protein-protein interaction (2009)
  • Author(s): Ishikawa, Y. Ito, Y. Sato, Y. Fukaya, M. Takahashi, H. Morikawa, N. Ohtake, T. Ohyam and K. Sueyoshi
  • Journal Title: Plant Biotechnol Volume: vol.26 Pages: 197-205
  • NAID: 10025181823
  • Peer Reviewed
• [Presentation] オオムギ硝酸トランスポーター研究の新たな展開 (2012)
  • Author(s): 末吉邦
  • Organizer: 第4回植物ストレス科学シンポジウム
  • Place of Presentation: 岡山大学資源植物科学研究所(招待講演)
  • Year and Date: 2012-03-09
• [Presentation] 植物トランスポーター研究の新しい展開 (2012)
  • Author(s): 末吉邦
  • Organizer: 第4回ストレス科学シンポジウム
  • Place of Presentation: 倉敷,岡山
  • Year and Date: 2012-03-08
• [Presentation] オオムギ低親和性硝酸トランスポーターの機能解析 (2011)
  • Author(s): 石川伸二・園部和幸・三谷奈見季・馬建鋒・原田久富美・大山卓爾・末吉邦
  • Organizer: 日本土壌肥料学会2011年つくば大会
  • Place of Presentation: つくば,茨城
  • Year and Date: 2011-08-09
• [Presentation] Nitrate transporters in barley (2011)
  • Author(s): Sueyoshi K
  • Organizer: 3rd Japan-China Joint Workshop on Plant Nutrition
  • Place of Presentation: 倉敷,岡山
  • Year and Date: 2011-03-27
• [Presentation] Nitrate transporters in barley (2011)
  • Author(s): 末吉邦、石川伸二
  • Organizer: 3rd Japan-China Join Workshop on Plant Nutrition
  • Place of Presentation: 倉敷市芸文館
  • Year and Date: 2011-03-27
• [Presentation] Two-component high-affinity nitrate transport system in barley : Localization in roots and protein-protein interaction in plant cells (2010)
  • Author(s): Ishikawa, S, Ohishi M, Yamaji N, Ma JF, Ohtake N, Ohyama T, Sueyoshi K
  • Organizer: 1st International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: 犬山,愛知
  • Year and Date: 2010-07-27
• [Presentation] Nitrate uptake, transport and accumulation in higher plants (2009)
  • Author(s): Sueyoshi K, Ohyama T
  • Organizer: 2nd International Meeting of IPM
  • Place of Presentation: ハノイ,ベトナム
  • Year and Date: 2009-12-09
• [Book] Nitrogen assimilation in plants (2010)
  • Author(s): 大山卓爾、末吉邦(編)
  • Total Pages: 378
  • Publisher: Research Signpost, Kerala, India
• [Remarks]
  • URL: http://www.agr.niigata-u.ac.jp/~sueyoshi/index.html
• [Remarks]
  • URL: http://www.agr.niigata-u.ac.jp/profile/sueyoshi/index.html