| Project/Area Number |
21580080
|
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| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
|
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| Research Institution | Osaka University (2010-2011)
Yokohama City University (2009) |
|---|
Principal Investigator |
|
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| Co-Investigator(Renkei-kenkyūsha) |
NAGATA Noriko 日本女子大学, 理学部, 准教授 (40311352)
ISSHIKI Masayuki 横浜市立大学, 木原生物学研究所, 准教授 (10314543)
KAWAMURA Kanako 横浜市立大学, 木原生物学研究所, 助教 (60381935)
OYAMA Kiyoshi 東京工業大学, 助教 (20391899)
|
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
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| Keywords | ステロール / 代謝工学 / コムギ / P450 / 同祖遺伝子 |
|---|
| Research Abstract |
Wheat(Triticum aestivum) is one of the three major crops. It is expected to add value to the crop by the modification of sterol profiles. However, because of the complicated genome(hexaploid) and difficulty of transgenic work, there have been no information of sterol pathway in the crop. In this study, 13 genes for putative sterol metabolic pathway was identified. Among these genes, we elucidated the CYP710A8 encoding sterol C-22 desaturase as a key characteristic for the higher level of stigmasterol. The gene was mapped on chromosome 3(3A, 3B, and 3D) and difference of the function of the genes was confirmed by overexpression of each gene in Arabidopsis.
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