Developmentally regulated gene reconstitution mediated by a phage DNA

• Project/Area Number: 21580087
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Hosei University
• Principal Investigator: SATO Tsutomu 法政大学, 生命科学部, 教授 (70215812)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細菌 / ファージ / DNA再編成 / 細胞分化 / 枯草菌 / 遺伝子発現

Research Abstract

The developmentally regulated gene spoVFB which encodes a dipicolinic acid synthetase was interrupted by a phage-like DNA in Bacillus weihenstephanensis KBAB4.We showed that the spoVFB gene is a composite of two truncated genes named 5'-spoVFB and 3'-spoVFB, which are brought together by site-specific recombination during sporulation.

Research Products

• [Journal Article] Repression of sigK intervening(skin) element gene expression by the cI-like protein SknR and effect of SknR depletion on growth of Bacillus subtilis cells (2010)
  • Author(s): Tatsu Kimura, Yukie Amaya, Kazuo Kobayashi, Naotake Ogasawara, Tsutomu Sato
  • Journal Title: Journal of Bacteriology Volume: 192巻 Pages: 6209-6216
  • Peer Reviewed
• [Journal Article] Repression of sigK intervening (skin) element gene expression by the cI-like protein SknR and effect of SknR depletion on growth of Bacillus subtilis cells (2010)
  • Author(s): Tatsu Kimura
  • Journal Title: Journal of Bacteriology Volume: 192 Pages: 6209-6216
  • Peer Reviewed
• [Presentation] Bacillus weihenstephanensis KBAB4株の胞子形成期におけるDNA再編成 (2012)
  • Author(s): 安部公博、青柳隆大、廣田泰伯、佐藤勉
  • Organizer: 日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学(東京都)
  • Year and Date: 2012-03-11
• [Presentation] Bacillus weihenstephanensis KBAB4株の胞子形成期におけるDNA再編成 (2012)
  • Author(s): 安部公博、佐藤勉, ら
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 立教大学
  • Year and Date: 2012-03-11
• [Presentation] 有胞子細菌の胞子形成期におけるDNA再編成 (2011)
  • Author(s): 佐藤勉、青柳隆大、安部公博
  • Organizer: グラム陽性菌ゲノム機能会議
  • Place of Presentation: 福山大学(広島県)
  • Year and Date: 2011-08-26
• [Presentation] 枯草菌胞子形成期の遺伝子再編成を仲介するskin elementの構造 (2010)
  • Author(s): 佐藤勉
  • Organizer: 微生物研究会
  • Place of Presentation: 法政大学(東京都)
  • Year and Date: 2010-06-26
• [Presentation] 枯草菌胞子形成期の遺伝子再編成を仲介するskin elementの構造 (2010)
  • Author(s): 木村達、佐藤勉
  • Organizer: 日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学医学部(福岡県)
  • Year and Date: 2010-03-08
• [Presentation] 枯草菌胞子形成期の遺伝子再編成を仲介するskin elementの構造 (2010)
  • Author(s): 木村達、佐藤勉
  • Organizer: ゲノム微生物学会
  • Place of Presentation: 九州大学医学部
  • Year and Date: 2010-03-08
• [Presentation] 枯草菌skin elementの遺伝子発現と胞子形成細菌のskinの遺伝子構成 (2009)
  • Author(s): 佐藤勉
  • Organizer: グラム陽性菌ゲノム機能会議
  • Place of Presentation: 神戸セミナーハウス(神戸市)
  • Year and Date: 2009-09-05
• [Presentation] 枯草菌skin elementの遺伝子発現と胞子形成細菌のskinの遺伝子構成 (2009)
  • Author(s): 佐藤勉
  • Organizer: グラム陽性菌ゲノム機能会議
  • Place of Presentation: 神戸セミナーハウス
  • Year and Date: 2009-09-05