Functional analysis of GGDEF/EAL proteins that control the stability of small RNA

• Project/Area Number: 21580088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Niigata University
• Principal Investigator: SUZUKI Kazushi 新潟大学, 自然科学系, 准教授 (00444183)
• Research Collaborator: TONY Romeo フロリダ大学, 微生物・細胞科学, 教授
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 大腸菌 / バイオフィルム / GGDEFドメイン / EALドメイン / c-di-GMP / Csrシステム / small RNA / c-fi-GMP

Research Abstract

To elucidate the mechanism of bacterial biofilm formation control, we analyzed the function of the GGDEF/EAL proteins in bacteria. GGDEF/EAL protein CsrD was not involved in c-di-GMP metabolism and altered the activity in each growth phase and controlled biofilm formation by regulating the stability of small RNA. On the other hand, YliF(GGDEF protein) and YliE(EAL protein) controlled biofilm formation by regulating the synthesis and degradation of c-di-GMP.

Research Products

• [Presentation] 大腸菌におけるGGDEF-EALドメインタンパク質CsrDによるsmall RNA半減期の調節 (2012)
  • Author(s): 高橋優介,岡山明裕,伊藤学,杉本華幸,渡邉剛志,鈴木一史
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2012-03-24
• [Presentation] 大腸菌におけるGGDEF/EALドメインタンパク質の機能解析 (2012)
  • Author(s): 塚田海斗,渡部大樹,杉本華幸,渡邉剛志,鈴木一史
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2012-03-24
• [Presentation] 大腸菌におけるCsrB RNA分解中間産物の解析 (2010)
  • Author(s): 鈴木一史,岡山明裕,伊藤学,渡邉剛志, Tony Romeo
  • Organizer: 第12回日本RNA学会
  • Place of Presentation: 一橋記念講堂(東京都千代田区)
  • Year and Date: 2010-07-28
• [Presentation] 大腸菌におけるCsrB RNA分解中間産物の解析 (2010)
  • Author(s): 鈴木一史
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 一橋記念講堂(東京都)
  • Year and Date: 2010-07-28
• [Presentation] Analysis of turnover of the global regulatory RNA CsrB in Escherichia coli (2010)
  • Author(s): 鈴木一史,岡山明裕,伊藤学,渡邉剛志, Tony Romeo
  • Organizer: American Society for Microbiology 110th General Meeting
  • Place of Presentation: San Diego Convebtion Center(San Diego, California,アメリカ合衆国)
  • Year and Date: 2010-05-26
• [Presentation] Analysis of turnover of the global regulatory RNA CsrB in Escherichia coli (2010)
  • Author(s): 鈴木一史
  • Organizer: American Society for Microbiology 110th General Meeting
  • Place of Presentation: San Diego Convention Center(San Diego, CA, USA)
  • Year and Date: 2010-05-26
• [Presentation] 大腸菌CsrB RNAの分解機構 (2010)
  • Author(s): 鈴木一史
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-29
• [Presentation] 大腸菌CsrB RNAの分解機構 (2010)
  • Author(s): 鈴木一史,伊藤学,岡山明裕,水野孝彦,渡邉剛志, Tony Romeo
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 名城大学(名古屋市)
  • Year and Date: 2010-03-28
• [Presentation] Analysis of CsrB small RNA decay in Escherichia coli (2009)
  • Author(s): Kazushi Suzuki, Manabu Ito, Takeshi Watanabe, and Tony Romeo
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-12
• [Presentation] Analysis of CsrB small RNA decay in Escherichia coli (2009)
  • Author(s): 鈴木一史
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Remarks]
  • URL: http://www.agr.niigata-u.ac.jp/~ksuzuki/ApplMicro/Welcome.html
• [Remarks]
  • URL: http://www.agr.niigata-u.ac.jp/~ksuzuki/ApplMicro/Welcome.html
• [Remarks]
  • URL: http://www.agr.niigata-u.ac.jp/~ksuzuki/index.html
• [Remarks]
  • URL: http://www.agr.niigata-u.ac.jp/profile/suzuki/index.html