| Project/Area Number |
21580089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Shinshu University |
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Principal Investigator |
IKEDA Masato 信州大学, 農学部, 教授 (00377649)
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| Co-Investigator(Kenkyū-buntansha) |
TAKENO Seiki 信州大学, 農学部, 助教 (30422702)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 発酵生産 / コリネバクテリウム・グルタミクム / 低酸素適応性 / シチジル酸キナーゼ / リボヌクレオチドレダクターゼ / GapN / Rho / グリセルアルデヒド-3-リン酸デヒドロゲナーゼ / 微好気生育 |
|---|
| Research Abstract |
We identified six genes relevant to the microaerobic growth of Corynebacterium glutamicum ; Cgl0807, Cgl1102, Cgl0600, Cgl1427, Cgl2857, and Cgl2859. Among these, the Cgl1427 gene encoding cytidylate kinase was shown to play an important role in DNA synthesis when cells were grown under low oxygen concentrations, suggesting that DNA synthesis might become a limiting factor for growth under oxygen limitation. Meanwhile, from the viewpoint of reducing oxygen requirement itself, we engineered a C. glutamicum strain with a modified glycolytic pathway that can generate NADPH instead of NADH, and showed its potential as a host for biotechnological processes.
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