| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
The conjugative transfer of the small plasmid takes a main role in horizontal gene transfer in Streptomyces. The small criptic plasmid, pSN22, from Streptomyces nigrifaciens can mobilize the host chromosome along with the plasmid transfer. We identified both the TraB protein is essential for the plasmid and genome transfer through cytoplasmic membrane and the ATP activity is essential for the DNA channel function. In addition the elements acted in trans, we also identified cis element essential for the plasmid transfer and determined the distinctive structure.
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