| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Disulfide bonds, formed between pairs of cysteines, are important for the folding,activity, and stability of many secretory and membrane proteins. In a bacterium Escherichia coli, an enzyme called DsbA introduces disulfide bonds into folding proteins in the periplasm. In this study, I detected, in vivo, the intermediates of disulfide bond formation that were formed between DsbA and its substrates. Detailed characterization of the intermediates revealed a number of new insights into protein folding in this cellular compartment.
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