Identification of glycosyltransferases that regulate morphology and differentiation of filamentous fungus
| Project/Area Number |
21580096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyushu University |
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Principal Investigator |
GOTO Masatoshi 九州大学, 大学院・農学研究院, 准教授 (90274521)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEGAWA Kaoru 九州大学, 大学院・農学研究院, 教授 (50197282)
FUTAGAMI Taiki 九州大学, 大学院・農学研究院, 助教 (60512027)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 糸状菌 / Aspergillus / O-結合型糖鎖 / 糖転移酵素 |
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| Research Abstract |
Aspergillus nidulans possesses three putativeα-1, 2-mannosyltransferases(Mnt) that are involved in protein O-glycosylation. We attempted to identify and characterize three Mnts of A. nidulans. The structure of O-glycans and mannosyltransferase activity using wt strain and three mnt-disruptants were determined. A. nidulans mnts(Anmnts) respectively expressed in a triple mnt mutant of yeast. The results indicate that AnMntA and AnMntC are not involved in O-glycosylation unlike to yeast Mnts, and that AnMntB functions in part similar to yeast Mnts. We determined whether AnMnts catalyze the synthetic reaction of the cell wall component, galactomannan that is present in Aspergilli. The results indicate that three AnMnts may be involved in synthesis ofα-1, 2-mannan polymer in galactomannan.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Takashita, H., Omori, T., Furukawa, K., Goto, M. Putative stress sensors WscA and WscB are involved in hypo-osmotic and acidic pH stress in Aspergillus nidulans2011
Author(s)
Futagami, T., Nakao, S., Kido, Y., Oka, T., Kajiwara, Y.
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Journal Title
Eukaryotic Cell
Volume: 10
Issue: 11
Pages: 1504-1515
DOI
Related Report
Peer Reviewed
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[Journal Article] Furukawa, K. Protein O-mannosyltransferase B and C support hyphal development and differentiation in Aspergillus nidulans2009
Author(s)
Goto, M., Harada, Y., Oka, T., Matsumoto, S., Takegawa, K.
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Journal Title
Eukaryotic Cell
Volume: 8
Issue: 10
Pages: 1465-1474
DOI
Related Report
Peer Reviewed
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