Studies on imbalance localization of phospholipids in the cellular membrane

• Project/Area Number: 21580099
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kagoshima University
• Principal Investigator: TAMAKI Hisanori 鹿児島大学, 農学部, 准教授 (20212045)
• Co-Investigator(Renkei-kenkyūsha): MIYASHITA Masahiro 京都大学, 大学院・農学研究科, 助教 (80324664)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: アシル転移酵素 / 脂質の不均衡分布 / 酵母 / 細胞膜リン脂質 / アシル転移酸素 / 脂質不均衡分布

Research Abstract

In this study, we clarify the relation between imbalance localization of double-bond in the phospholipids and newly found lyso-phospholipid acyltransferases. Two acyltransferases Lpt1 and Slc1 were purified(or partially purified) and substrate specificities were examined. Also total lipids were extracted from lpt1 or slc1 gene disrupted strain and subjected to LC-MS/MS analysis. From these experiments, Two enzymes mainly catalyze introduction of acyl-residue containing double-bond, although substrate preference were different.

Research Products

• [Journal Article] Development of a heat-processing method for koji to enhance its antioxidant activity (2012)
  • Author(s): Okutsu, K., Yoshizaki, Y., Takamine, K., Tamaki, H., Ito, K., and Sameshima, Y.
  • Journal Title: J. Biosci. Bioeng. Volume: 113(3) Pages: 349-354
  • NAID: 110009425326
  • Peer Reviewed
• [Journal Article] Kluyveromyces marxianus-based Platform for Direct Ethanol Fermentation and Recovery from Cellulosic Materials Under Air-Ventilated Condition (2012)
  • Author(s): Matsuzaki, C, Nakagawa, A., Koyanagi, T., Tamaki, H., et al
  • Journal Title: J.Biosci.Bioeng. Volume: 113 Issue: 5 Pages: 604-607
  • DOI: 10.1016/j.jbiosc.2011.12.007
  • NAID: 110009456899
  • Peer Reviewed
• [Journal Article] Development of a heat-processing method for koji to enhance its antioxidant activity (2012)
  • Author(s): Okutsu, K., Yoshizaki, Y., Takamine, K., Tamaki, H., Ito, K., Sameshima, Y.
  • Journal Title: J.Biosci.Bioeng. Volume: 113(3) Issue: 3 Pages: 349-354
  • DOI: 10.1016/j.jbiosc.2011.10.024
  • NAID: 110009425326
  • Peer Reviewed
• [Journal Article] The formation of beta-damascenone in sweet potato shochu (2011)
  • Author(s): Yoshizaki, Y., Takamine, K., Shimada, S., Uchihori, K., Okutsu, K., Tamaki, H., Ito, K., and Sameshima Y.
  • Journal Title: J. Inst. Brew Volume: 117 Pages: 217-223
  • Peer Reviewed
• [Journal Article] The formation of beta-damascenone in sweet potato shochu (2011)
  • Author(s): Yoshizaki, Y., Takamine, K., Shimada, S., Uchihori, K., Okutsu, K., Tamaki, H., et al
  • Journal Title: J.Inst.Brew. Volume: 117 Pages: 217-223
  • Peer Reviewed
• [Journal Article] Role of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3β-glucosidase from Kluyveromyces marxianus (2010)
  • Author(s): Yoshida, E., Hidaka, M., Fushinobu, S., Koyanagi, T., Minami, H., Tamaki, H., Kitaoka, M., Katayama, T., and Kumagai, H.
  • Journal Title: Biochem. J. Volume: 431(1) Pages: 39-49
  • Peer Reviewed
• [Journal Article] Characterization of glucoamylase and α-amylase from Monascus anka : Enhanced production of α-amylase in red koji. (2010)
  • Author(s): Yoshizaki, Y., Susuki, T., Takamine, K., Tamaki, H., Ito, K., Sameshima Y.
  • Journal Title: J.Biosci.Bioeng. Volume: 110 Pages: 670-674
  • NAID: 110008143501
  • Peer Reviewed
• [Journal Article] Role of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3 β-glucosidase from Kluyveromyces marxianus. (2010)
  • Author(s): Yoshida, E., Tamaki, H., Kitaoka, M., Katayama, T., Kumagai, H., et al.
  • Journal Title: Biochem.J. Volume: 431 Pages: 39-49
  • Peer Reviewed
• [Journal Article] Analysis of Volatile Compounds in Shochu Koji, Sake Koji, and Steamed Rice by Gas Chromatography-Mass Spectrometry. (2010)
  • Author(s): Yoshizaki, Y., Yamato, H., Takamine, K.Tamaki, H., Ito K., Sameshima Y.
  • Journal Title: J.Inst.Brew. Volume: 116 Pages: 49-55
  • Peer Reviewed
• [Journal Article] Purification, crystallization and preliminary X-ray analysis of β-glucosidase from Kluyveromyces marxianus NBRC1777 (2009)
  • Author(s): Yoshida, E., Tamaki, H., Katayama, T., Kumagai, H., et.al
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun 65 Pages: 1190-1192
  • Peer Reviewed
• [Presentation] 分裂酵母リゾリン脂質アシル転移酵素Slc1の機能解析 (2011)
  • Author(s): 福田竜也, 吉崎由美子, 高峯和則, 鮫島吉廣, 伊藤清, 玉置尚徳
  • Organizer: Yeast Workshop
  • Place of Presentation: 徳島文理大学
  • Year and Date: 2011-11-12
• [Presentation] 分裂酵母リゾリン脂質アシル転移酵素Slc1の機能解析 (2011)
  • Author(s): 福田竜也
  • Organizer: 第44回酵母遺伝学フォーラム
  • Place of Presentation: 福岡、九大医学部百年講堂
  • Year and Date: 2011-09-06
• [Presentation] 「酵母をとおして見えるもの」-焼酎作りから生命原理の解明まで- (2011)
  • Author(s): 玉置尚徳
  • Organizer: 常盤台コロキアム
  • Place of Presentation: 山口大学
  • Year and Date: 2011-03-09
• [Presentation] 焼酎酵母K2株における遺伝子破壊システムの構築 (2010)
  • Author(s): 田平愛美、中山恵利、下島彩音、玉置尚徳
  • Organizer: 第28回イーストワークショップ
  • Place of Presentation: 福山
  • Year and Date: 2010-11-13
• [Presentation] 酵母アシルトランスフェラーゼの機能解析 (2010)
  • Author(s): 玉置尚徳
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 群馬、伊香保温泉
  • Year and Date: 2010-06-15
• [Presentation] 酵母アシルトランスフェラーゼの機能解析 (2010)
  • Author(s): 玉置尚徳、中山玲子、熊谷英彦
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 伊香保(群馬)
  • Year and Date: 2010-06-15
• [Presentation] 耐熱性酵母を用いたセルロース資化系の開発 (2010)
  • Author(s): 玉置尚徳
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-30
• [Presentation] LOHを利用した焼酎酵母ホストベクター系の開発 (2009)
  • Author(s): 中山恵利, 玉置尚徳, 他3名
  • Organizer: イーストワークショップ
  • Place of Presentation: 飯塚研究開発センター(福岡)
  • Year and Date: 2009-11-14
• [Presentation] 有用酵素生産システム構築に向けた酵母の分子育種 (2009)
  • Author(s): 玉置尚徳
  • Organizer: 日本農芸化学会(合同沖縄大会)
  • Place of Presentation: ロワジールホテル那覇
  • Year and Date: 2009-10-30
• [Presentation] 酵母新規アシル転移酵素の機能解析 (2009)
  • Author(s): 玉置尚徳
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸、ポートピアホテル
  • Year and Date: 2009-10-24
• [Presentation] 新規アシル転移酵素の機能解析 (2009)
  • Author(s): 玉置尚徳
  • Organizer: 日本生化学会
  • Place of Presentation: ポートピアホテル(神戸)
  • Year and Date: 2009-10-24
• [Presentation] 焼酎酵母分子育種のための基盤システムの構築 (2009)
  • Author(s): 中山恵利, 玉置尚徳, 他6名
  • Organizer: 日本醸造学会
  • Place of Presentation: 北とぴあつつじホール(東京)
  • Year and Date: 2009-09-18
• [Presentation] 細胞膜リン脂質のリモデリングを担う新規アシル転移酵素の機能解析 (2009)
  • Author(s): 玉置尚徳
  • Organizer: 蛋白質と酵素の構造と機能に関する九州シンポジウム
  • Place of Presentation: 唐津シーサイドホテル(佐賀)
  • Year and Date: 2009-09-12
• [Presentation] LOHを利用した焼酎酵母ホストベクター系の構築 (2009)
  • Author(s): 中山恵利, 玉置尚徳, 他6名
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: ノバホール(つくば)
  • Year and Date: 2009-07-29
• [Presentation] Role of lysophospholipid acyltransferase in the fission yeast (2009)
  • Author(s): 玉置尚徳, 他5名
  • Organizer: 4^<th>.International conference on phospholipase A2 and lipid mediators
  • Place of Presentation: Tokyo
  • Year and Date: 2009-05-26