Development of Pro-biotic Lactic Acid Bacteria Scavenging Environmental Hydroperoxides

• Project/Area Number: 21580101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: NIIMURA Youichi 東京農業大学, 応用生物科学部, 教授 (00180563)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 乳酸菌 / プロバイオティックス / 過酸化脂質 / 過酸化脂質分解 / 過酸化水素 / 過酸化水素分解 / Mg-catalase / NADH peroxidase

Research Abstract

In order to get lactic acid bacteria scavenging environmental lipid peroxide, we successfully isolated Lactobacillus plantarum P2 strain and purified two lipid peroxide reductases from the bacteria. The enzymes catalyze the reduction of lipid peroxide to its alcohol derivative with NAD(P) H as the preferred electron donor. The gene of the enzyme was cloned and overexpressed in E. coli. The recombinant enzyme has been purified to homogeneity and analyzed to get enzymatic parameters.

Research Products

• [Journal Article] Lactobacillus ozensis sp. nov., a novel lactic acid bacteria isolated from mountain flowers in Oze National Park (2011)
  • Author(s): Kawasaki S, Kurosawa K, Miyazaki M, Sakamoto M, Ohkuma M, Y. Niimura
  • Journal Title: It. J. Syst. Evol.Microbiol Volume: 61巻 Pages: 2435-2438
  • Peer Reviewed
• [Journal Article] Lactobacillus floricola sp. nov., a novel lactic acid bacteria isolated from mountain flowers in Japan (2011)
  • Author(s): Kawasaki S, Kurosawa K, Miyazaki M, Yagi C, Kitajima Y, Tanaka S, Irisawa T, Okada S, Sakamoto M, Ohkuma M, Y. Niimura
  • Journal Title: It. J. Syst. Evol.Microbiol Volume: 61巻 Pages: 1356-1359
  • Peer Reviewed
• [Journal Article] Synechocystis ferredoxin-NADP^+oxidoreductase is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin (2011)
  • Author(s): Junichi S., K. Takeda, R. Nishiyama, T. Watanabe, M. Abo, E. Yoshimura, J. Nakagawa, A. Abe, S. Kawasaki, Y. Niimura
  • Journal Title: Biometals Volume: 24巻 Pages: 311-321
  • Peer Reviewed
• [Journal Article] Lactobacillus ozensis sp.nov., a novel lactic acid bacteria isolated from mountain flowers in Oze National Park (2011)
  • Author(s): Kawasaki S, Kurosawa K, Miyazaki M, Sakamoto M, Ohkuma M, Y.Niimura
  • Journal Title: It.J.Syst.Evol.Microbiol Volume: 61巻 Issue: 10 Pages: 2435-2438
  • DOI: 10.1099/ijs.0.027847-0
  • Peer Reviewed
• [Journal Article] Lactobacillus floricola sp.nov., a novel lactic acid bacteria isolated from mountain flowers in Japan (2011)
  • Author(s): Kawasaki S, Kurosawa K, Miyazaki M, Yagi C, Kitajima Y, Tanaka S, Irisawa T, Okada S, Sakamoto M, Ohkuma M, Y.Niimura
  • Journal Title: It.J.Syst.Evol.Microbiol Volume: 61巻 Issue: 6 Pages: 1356-1359
  • DOI: 10.1099/ijs.0.022988-0
  • Peer Reviewed
• [Journal Article] Synechocystis ferredoxin-NADP^+ oxidoreductase is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin (2011)
  • Author(s): Junichi S., K.Takeda, R.Nishiyama, T.Watanabe, M.Abo, E.Yoshimura, J.Nakagawa, A.Abe, S.Kawasaki, Y.Niimura
  • Journal Title: Biometals Volume: 24巻 Pages: 311-321
  • Peer Reviewed
• [Journal Article] Escherichia coli ferredoxin-NADP^+reductase and oxygen-insensitive NAD(P) H nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction (2010)
  • Author(s): K. Takeda, J. Sato, K. Goto, T. Fujita, T. Watanabe, M. Abo, E. Yoshimura, J. Nakagawa, A. Abe, S. Kawasaki, Y. Niimura
  • Journal Title: Biometals Volume: 23巻 Pages: 727-737
  • Peer Reviewed
• [Journal Article] Chlorella vulgaris aldehyde reductase is capable of functioning as ferric reductase and of driving the Fenton react ion in the presence of free flavin (2010)
  • Author(s): J. Sato, K. Takeda, R. Nishiyama, K. Fusayama, T. Arai, T. Sato, T. Watanabe, A. Abe, J. Nakagawa, S. Kawasaki, Y. Niimura
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 74巻 Pages: 854-857
  • NAID: 10027554231
  • Peer Reviewed
• [Journal Article] Crystal structure of NADH : rubredoxin oxidoreductase from Clostridium. acetobutylicum : a key component of the dioxygen scavenging system in obligatory anaerobes (2010)
  • Author(s): Nishikawa K., Shomura Y., Kawasaki S., Y. Niimura., H. Yoshiki.
  • Journal Title: Proteins Volume: 78巻 Pages: 1066-1066
  • Peer Reviewed
• [Journal Article] Escherichia coli ferredoxin-NADP^+ reductase and oxygen-insensitive NAD(P)H nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction (2010)
  • Author(s): K.Takeda, J.Sato, K.Goto, T.Fujita, T.Watanabe, M.Abo, E.Yoshimura, J.Nakagawa, A.Abe, S.Kawasaki, Y.Niimura
  • Journal Title: Biometals Volume: 23巻 Pages: 727-737
  • Peer Reviewed
• [Journal Article] Sodium Acetate Enhances Hydrogen Peroxide Production in Weissella cibaria (2009)
  • Author(s): A. Endo, Y. Futagawa-Endo, S. Kawasaki, Leon M. T. Dicks, Y. Niimura & S. Okada
  • Journal Title: Letters in Applied Microbiology-LAN
  • Peer Reviewed
• [Journal Article] Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family (2009)
  • Author(s): N. Shibata, Y. Ueda, D. Takeuchi, Y. Haruyama, S. Kojima, J. Sato, Y. Niimura, M. Kitamura, Y. Higuchi
  • Journal Title: FEBS Journal Volume: 276巻 Pages: 4840-4853
  • Peer Reviewed
• [Journal Article] O_2 and reactive oxygen species detoxification complex, composed of O_2-responsive NADH : rubredoxin oxidoreductase-flavoprotein A2-desulfoferrodoxin operon enzymes, rubperoxin, and rubredoxin, in Clostridium acetobutylicum (2009)
  • Author(s): Kawasaki, S, Y. Sakai, T. Takahashi, I. Suzuki and Y. Niimura
  • Journal Title: Appl. Environ. Microbiol. Volume: 75巻 Pages: 1021-1029
  • Peer Reviewed
• [Journal Article] O_2 and reactive oxygen species detoxification complex, composed of O_2-responsive NADH : rubredoxin oxidoreductase-flavoprotein A2-desulfoferrodoxin operon enzymes, rubperoxin, and rubredoxin, in Clostridium acetobutylicum. (2009)
  • Author(s): Kawasaki, S, Y.Sakai, T.Takahashi, I.Suzuki, Y.Niimura
  • Journal Title: Appl.Environ.Microbiol. Volume: 75 Pages: 1021-1029
  • Peer Reviewed
• [Journal Article] b-type dihydroorotate dehydrogenase is purified as a H_2O_2-forming NADH oxidase from Bifidobacterium bifidum. (2009)
  • Author(s): Kawasaki, S., T.Satoh, M.Todoroki, Y.Niimura.
  • Journal Title: Appl.Environ.Microbiol. Volume: 75 Pages: 629-636
  • Peer Reviewed
• [Journal Article] Taxonomical and physiological comparisons of the three species of the genus Amphibacillus (2009)
  • Author(s): Toshiaki Arai, Shuhei Yanahashi, Junichi Sato, Takumi Sato, Morio Ishikawa, Yukimichi Koizumi, Shinji Kawasaki, Youichi Niimura, Junichi Nakagawa
  • Journal Title: Journal of General and Applied Microbiology Volume: 55 Pages: 155-162
  • NAID: 130004802134
  • Peer Reviewed
• [Journal Article] Expression Pattern of a Chloroplast NADPH-Dependent Thioredoxin Reductase in Chlorella vulgaris during Hardening and Its Interaction with 2-Cys Peroxiredoxin (2009)
  • Author(s): Takeshi MACHIDA, Eri KATO, Akiko ISHIBASHI, Jun-ichi SATO, Shinji KAWASAKI, Youichi NIIMURA, Ken-ichi HONJOH, Takahisa MIYAMOTO
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 73 Pages: 695-701
  • NAID: 10027538998
  • Peer Reviewed
• [Journal Article] Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family (2009)
  • Author(s): Naoki Shibata, Yasufumi Ueda, Daisuke Takeuchi, Yoshihiro Haruyama, Shuichi Kojima, Junichi Sato, Youichi Niimura, Masaya Kitamura, Yoshiki Higuchi
  • Journal Title: FEBS Journal Volume: 276 Pages: 4840-4853
  • Peer Reviewed
• [Journal Article] Sodium Acetate Enhances Hydrogen Peroxide Production in Weissella ciboria (2009)
  • Author(s): A.Endo, Y.Futagawa-Endol, S.Kawasaki, L.M.T.Dicks, Y.Niimura, S.Okadal
  • Journal Title: Applied Microbiology Volume: LAM
  • Peer Reviewed
• [Presentation] 微生物の過酸化物分解酵素の多様性 (2011)
  • Author(s): 新村洋一、望月大地、新井俊晃、大山修平、佐藤純一、川崎信治、渡邊昭夫
  • Organizer: 生体触媒化学シンポジウム
  • Place of Presentation: 慶應義塾大学薬学部
  • Year and Date: 2011-12-21
• [Presentation] 過酸化物分解する乳酸菌の開発 (2011)
  • Author(s): 渡邊昭夫、大山修平、佐藤純一、新村洋一
  • Organizer: 生体触媒化学シンポジウム
  • Place of Presentation: 慶應義塾大学薬学部
  • Year and Date: 2011-12-21
• [Presentation] 微生物の過酸化物分解酵素の多様性 (2011)
  • Author(s): 新村洋一、望月大地、新井俊晃、大山修平、佐藤純一、川崎信治、渡邊昭夫
  • Organizer: 生体触媒学会シンポジウム
  • Place of Presentation: 慶応大学薬学部(東京)(招待講演)
  • Year and Date: 2011-12-21