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Development of Pro-biotic Lactic Acid Bacteria Scavenging Environmental Hydroperoxides

Research Project

Project/Area Number 21580101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionTokyo University of Agriculture

Principal Investigator

NIIMURA Youichi  東京農業大学, 応用生物科学部, 教授 (00180563)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords乳酸菌 / プロバイオティックス / 過酸化脂質 / 過酸化脂質分解 / 過酸化水素 / 過酸化水素分解 / Mg-catalase / NADH peroxidase
Research Abstract

In order to get lactic acid bacteria scavenging environmental lipid peroxide, we successfully isolated Lactobacillus plantarum P2 strain and purified two lipid peroxide reductases from the bacteria. The enzymes catalyze the reduction of lipid peroxide to its alcohol derivative with NAD(P) H as the preferred electron donor. The gene of the enzyme was cloned and overexpressed in E. coli. The recombinant enzyme has been purified to homogeneity and analyzed to get enzymatic parameters.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2011 2010 2009

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (3 results)

  • [Journal Article] Lactobacillus ozensis sp. nov., a novel lactic acid bacteria isolated from mountain flowers in Oze National Park2011

    • Author(s)
      Kawasaki S, Kurosawa K, Miyazaki M, Sakamoto M, Ohkuma M, Y. Niimura
    • Journal Title

      It. J. Syst. Evol.Microbiol

      Volume: 61巻 Pages: 2435-2438

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lactobacillus floricola sp. nov., a novel lactic acid bacteria isolated from mountain flowers in Japan2011

    • Author(s)
      Kawasaki S, Kurosawa K, Miyazaki M, Yagi C, Kitajima Y, Tanaka S, Irisawa T, Okada S, Sakamoto M, Ohkuma M, Y. Niimura
    • Journal Title

      It. J. Syst. Evol.Microbiol

      Volume: 61巻 Pages: 1356-1359

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synechocystis ferredoxin-NADP^+oxidoreductase is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin2011

    • Author(s)
      Junichi S., K. Takeda, R. Nishiyama, T. Watanabe, M. Abo, E. Yoshimura, J. Nakagawa, A. Abe, S. Kawasaki, Y. Niimura
    • Journal Title

      Biometals

      Volume: 24巻 Pages: 311-321

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lactobacillus ozensis sp.nov., a novel lactic acid bacteria isolated from mountain flowers in Oze National Park2011

    • Author(s)
      Kawasaki S, Kurosawa K, Miyazaki M, Sakamoto M, Ohkuma M, Y.Niimura
    • Journal Title

      It.J.Syst.Evol.Microbiol

      Volume: 61巻 Issue: 10 Pages: 2435-2438

    • DOI

      10.1099/ijs.0.027847-0

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lactobacillus floricola sp.nov., a novel lactic acid bacteria isolated from mountain flowers in Japan2011

    • Author(s)
      Kawasaki S, Kurosawa K, Miyazaki M, Yagi C, Kitajima Y, Tanaka S, Irisawa T, Okada S, Sakamoto M, Ohkuma M, Y.Niimura
    • Journal Title

      It.J.Syst.Evol.Microbiol

      Volume: 61巻 Issue: 6 Pages: 1356-1359

    • DOI

      10.1099/ijs.0.022988-0

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synechocystis ferredoxin-NADP^+ oxidoreductase is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin2011

    • Author(s)
      Junichi S., K.Takeda, R.Nishiyama, T.Watanabe, M.Abo, E.Yoshimura, J.Nakagawa, A.Abe, S.Kawasaki, Y.Niimura
    • Journal Title

      Biometals

      Volume: 24巻 Pages: 311-321

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Escherichia coli ferredoxin-NADP^+reductase and oxygen-insensitive NAD(P) H nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction2010

    • Author(s)
      K. Takeda, J. Sato, K. Goto, T. Fujita, T. Watanabe, M. Abo, E. Yoshimura, J. Nakagawa, A. Abe, S. Kawasaki, Y. Niimura
    • Journal Title

      Biometals

      Volume: 23巻 Pages: 727-737

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chlorella vulgaris aldehyde reductase is capable of functioning as ferric reductase and of driving the Fenton react ion in the presence of free flavin2010

    • Author(s)
      J. Sato, K. Takeda, R. Nishiyama, K. Fusayama, T. Arai, T. Sato, T. Watanabe, A. Abe, J. Nakagawa, S. Kawasaki, Y. Niimura
    • Journal Title

      Bioscience Biotechnology and Biochemistry

      Volume: 74巻 Pages: 854-857

    • NAID

      10027554231

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of NADH : rubredoxin oxidoreductase from Clostridium. acetobutylicum : a key component of the dioxygen scavenging system in obligatory anaerobes2010

    • Author(s)
      Nishikawa K., Shomura Y., Kawasaki S., Y. Niimura., H. Yoshiki.
    • Journal Title

      Proteins

      Volume: 78巻 Pages: 1066-1066

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Escherichia coli ferredoxin-NADP^+ reductase and oxygen-insensitive NAD(P)H nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction2010

    • Author(s)
      K.Takeda, J.Sato, K.Goto, T.Fujita, T.Watanabe, M.Abo, E.Yoshimura, J.Nakagawa, A.Abe, S.Kawasaki, Y.Niimura
    • Journal Title

      Biometals

      Volume: 23巻 Pages: 727-737

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sodium Acetate Enhances Hydrogen Peroxide Production in Weissella cibaria2009

    • Author(s)
      A. Endo, Y. Futagawa-Endo, S. Kawasaki, Leon M. T. Dicks, Y. Niimura & S. Okada
    • Journal Title

      Letters in Applied Microbiology-LAN

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family2009

    • Author(s)
      N. Shibata, Y. Ueda, D. Takeuchi, Y. Haruyama, S. Kojima, J. Sato, Y. Niimura, M. Kitamura, Y. Higuchi
    • Journal Title

      FEBS Journal

      Volume: 276巻 Pages: 4840-4853

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] O_2 and reactive oxygen species detoxification complex, composed of O_2-responsive NADH : rubredoxin oxidoreductase-flavoprotein A2-desulfoferrodoxin operon enzymes, rubperoxin, and rubredoxin, in Clostridium acetobutylicum2009

    • Author(s)
      Kawasaki, S, Y. Sakai, T. Takahashi, I. Suzuki and Y. Niimura
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 75巻 Pages: 1021-1029

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] O_2 and reactive oxygen species detoxification complex, composed of O_2-responsive NADH : rubredoxin oxidoreductase-flavoprotein A2-desulfoferrodoxin operon enzymes, rubperoxin, and rubredoxin, in Clostridium acetobutylicum.2009

    • Author(s)
      Kawasaki, S, Y.Sakai, T.Takahashi, I.Suzuki, Y.Niimura
    • Journal Title

      Appl.Environ.Microbiol.

      Volume: 75 Pages: 1021-1029

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] b-type dihydroorotate dehydrogenase is purified as a H_2O_2-forming NADH oxidase from Bifidobacterium bifidum.2009

    • Author(s)
      Kawasaki, S., T.Satoh, M.Todoroki, Y.Niimura.
    • Journal Title

      Appl.Environ.Microbiol.

      Volume: 75 Pages: 629-636

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Taxonomical and physiological comparisons of the three species of the genus Amphibacillus2009

    • Author(s)
      Toshiaki Arai, Shuhei Yanahashi, Junichi Sato, Takumi Sato, Morio Ishikawa, Yukimichi Koizumi, Shinji Kawasaki, Youichi Niimura, Junichi Nakagawa
    • Journal Title

      Journal of General and Applied Microbiology

      Volume: 55 Pages: 155-162

    • NAID

      130004802134

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression Pattern of a Chloroplast NADPH-Dependent Thioredoxin Reductase in Chlorella vulgaris during Hardening and Its Interaction with 2-Cys Peroxiredoxin2009

    • Author(s)
      Takeshi MACHIDA, Eri KATO, Akiko ISHIBASHI, Jun-ichi SATO, Shinji KAWASAKI, Youichi NIIMURA, Ken-ichi HONJOH, Takahisa MIYAMOTO
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 73 Pages: 695-701

    • NAID

      10027538998

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family2009

    • Author(s)
      Naoki Shibata, Yasufumi Ueda, Daisuke Takeuchi, Yoshihiro Haruyama, Shuichi Kojima, Junichi Sato, Youichi Niimura, Masaya Kitamura, Yoshiki Higuchi
    • Journal Title

      FEBS Journal

      Volume: 276 Pages: 4840-4853

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sodium Acetate Enhances Hydrogen Peroxide Production in Weissella ciboria2009

    • Author(s)
      A.Endo, Y.Futagawa-Endol, S.Kawasaki, L.M.T.Dicks, Y.Niimura, S.Okadal
    • Journal Title

      Applied Microbiology

      Volume: LAM

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 微生物の過酸化物分解酵素の多様性2011

    • Author(s)
      新村洋一、望月大地、新井俊晃、大山修平、佐藤純一、川崎信治、渡邊昭夫
    • Organizer
      生体触媒化学シンポジウム
    • Place of Presentation
      慶應義塾大学薬学部
    • Year and Date
      2011-12-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 過酸化物分解する乳酸菌の開発2011

    • Author(s)
      渡邊昭夫、大山修平、佐藤純一、新村洋一
    • Organizer
      生体触媒化学シンポジウム
    • Place of Presentation
      慶應義塾大学薬学部
    • Year and Date
      2011-12-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 微生物の過酸化物分解酵素の多様性2011

    • Author(s)
      新村洋一、望月大地、新井俊晃、大山修平、佐藤純一、川崎信治、渡邊昭夫
    • Organizer
      生体触媒学会シンポジウム
    • Place of Presentation
      慶応大学薬学部(東京)(招待講演)
    • Year and Date
      2011-12-21
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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