Elucidation of reaction mechanism of novel biosynthetic enzymes for derivatization of reveromycin
Project/Area Number |
21580104
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied microbiology
|
Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
TAKAHASHI Shunji 独立行政法人理化学研究所, 化学情報・化合物創製チーム, チームヘッド (30311608)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 放線菌 / 天然化合物 / ポリケチド / リベロマイシンA / スピロアセタール化合物 / 生合成 / スピロアセタール / P450 |
Research Abstract |
We disrupted reveromycin biosynthetic gene cluster and analyzed the structure of biosynthetic intermediates isolated from each disruptant. We identified various reveromycin derivatives required for the speculation of its biosynthetic pathway. Moreover, the analysis of biosynthetic mechanisms using RM-A1a(triol intermediate) and recombinant enzymes resulted in the identification of dihydroxy ketone synthase(RevG) and spiroacetal synthase(RevJ), which are involved in stereospecific spiroacetal formation.
|
Report
(4 results)
Research Products
(92 results)
-
-
-
[Journal Article] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation2011
Author(s)
Takahashi S, Toyoda A, Sekiyama Y, Takagi H, Nogawa T, Uramoto M, Suzuki R, Koshino H, Kumano T, Panthee S, Dairi T, Ishikawa J, Ikeda H, Sakaki Y, and Osada H
-
Journal Title
Nat. Chem. Biol
Volume: 7
Pages: 461-468
Related Report
Peer Reviewed
-
-
[Journal Article] Genetic safeguard against mycotoxin cyclopiazonic acid production in Aspergillus oryzae2011
Author(s)
Kato N., Tokuoka M., Shinohara Y., Kawatani M., Uramoto M., Seshime Y., Fujii I., Kitamoto K., Takahashi T., Takahashi S., Koyama Y., and Osada H
-
Journal Title
Chembiochem
Volume: 12(9)
Pages: 1376-1382
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-