| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
We disrupted reveromycin biosynthetic gene cluster and analyzed the structure of biosynthetic intermediates isolated from each disruptant. We identified various reveromycin derivatives required for the speculation of its biosynthetic pathway. Moreover, the analysis of biosynthetic mechanisms using RM-A1a(triol intermediate) and recombinant enzymes resulted in the identification of dihydroxy ketone synthase(RevG) and spiroacetal synthase(RevJ), which are involved in stereospecific spiroacetal formation.
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