| Project/Area Number |
21580105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KOSONO Saori 独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員 (90321760)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Takehiro 独立行政法人理化学研究所, バイオ解析チーム, 技師 (60415198)
HIRAI Go 独立行政法人理化学研究所, 袖岡有機合成化学研究室, 専任研究員 (50359551)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タンパク質アセチル化 / アセチローム / 代謝酵素 / 翻訳関連因子 / リジン脱アセチル化酵素 / リジンアセチル化酵素 / 枯草菌 / コリネバクテリウム菌 / コリネバクテリウム / グルタミン酸生産 / タンパク質(リジン)アセチル化 / 枯草菌(Bacillus subtilis) / 必須タンパク質 / リン酸化プロテオーム / 脱アセチル化酵素 / プロテオミクス / シャペロン / HDAC |
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| Research Abstract |
To understand biological significance of protein(lysine) acetylation in bacteria, we performed acetylome analysis in Bacillus subtilis. We identified 145 acetylated peptides that matched to 120 proteins, which are involved in metabolism and translation as well as essential functions. We identified elongation factor(EF)-Tu as a major acetylated protein, acetylation level of which is differentially regulated in growth phases and medium conditions. We also performed acetylome analysis in Corynebacterium glutamicum.
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