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Studies on molecular targets of new biological compounds having activity against the mutant yeast involving calcium signaling

Research Project

Project/Area Number 21580108
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionIwate University

Principal Investigator

KIMURA Ken-ichi  岩手大学, 農学部, 教授 (30344625)

Co-Investigator(Kenkyū-buntansha) SHIONO Yoshihito  山形大学, 農学部, 准教授 (80361278)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsSaccharomyces cerevisiae (zds1Δerg3Δpdr1/3Δ) / Ca^<2+>-signaling / bemzophomopsin A / 9-(Methylsulfinyl) hexyl isothocyanate / falcarindiol / pisiferdiol / GSK-3β / calcineurin / Saccharomyces cerevisiae / Ca^<2+>-signal transduction / phenotype / eremoxylarin A / anthracobic acid A / benzophomopsin A / isothiocyanate / Eremoxylarine / Anthracobic acid / Benzophomopsin A
Research Abstract

A new compound, bemzophomopsin restored the growth activity of the yeast mutant strain (zds1Δ erg3Δ pdr1/3Δ) at 1.25μg/disc and has no inhibition activity against calcineurin at 100 μM. 9-(Methylsulfinyl) hexyl isothocyanate inhibited GSK-3β with ATP competitive manner on Ki = 10.5μM and falcarindiol inhibited GSK-3β with ATP non-competitive manner on Ki = 86.9μM and the peptide substrate competitive manner on K_i=46.5μM. Pisiferdiol decreased the expression of calcineurin in the mutant yeast.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (28 results)

All 2012 2011 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 2 results) Presentation (11 results) Book (2 results) Remarks (7 results)

  • [Journal Article] わさびとウド・セリ科野菜に含まれるGSK-3β阻害物質の2型糖尿病に関わる機能性2012

    • Author(s)
      吉田潤、伊藤芳明、木村賢一
    • Journal Title

      ニューフードインダストリー

      Volume: 54 Pages: 9-18

    • NAID

      120005249353

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Glycogen synthase kinase-3 β inhibition of 6-(methylsulfinyl) hexyl isothiocyanate derived from wasabi (Wasabia Japonica Matsum)2011

    • Author(s)
      Yoshida, J., Nomura, S. Nishizawa, N., Ito, Y. and Kimura, K.
    • Journal Title

      Biosci. Biochem. Biotechnol

      Volume: 75 Pages: 136-139

    • NAID

      10027897009

    • Related Report
      2011 Final Research Report
  • [Journal Article] 沢わさびの抗生活習慣病特性2011

    • Author(s)
      伊藤芳明、渡部達也、吉田潤、木村賢一、長澤孝志
    • Journal Title

      ニューフードインダストリー

      Volume: 53 Pages: 20-24

    • NAID

      120005249350

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Glycogen synthase kinase-3 β inhibition of 6(methylsulfinyl)hexyl isothiocyanate derived from wasabi(Wasabia Japonica Matsum)2011

    • Author(s)
      Yoshida, J., Nomura, S.Nishizawa, N., Ito, Y., Kimura, K.
    • Journal Title

      Biosci. Biochem. Biotechnol.

      Volume: 75 Pages: 136-139

    • NAID

      10027897009

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new benzoxepin metabolite isolated from endophytic fungus Phomopsis sp.2009

    • Author(s)
      Shiono, Y., Nitto, A., Shimanuki, K., Koseki, T., Murayama, T., Miyakawa, T., Yoshida, J. and Kimura, K.
    • Journal Title

      J. Antibiot

      Volume: 62 Pages: 533-535

    • NAID

      10030636414

    • Related Report
      2011 Final Research Report
  • [Journal Article] 植物内生糸状菌類から創薬シード分子を探す2009

    • Author(s)
      塩野義人、木村賢一
    • Journal Title

      化学と生物

      Volume: 47 Pages: 390-396

    • NAID

      10026435645

    • Related Report
      2011 Final Research Report
  • [Journal Article] 植物内生糸状菌類から創薬シード分子を探す2009

    • Author(s)
      塩野義人, 木村賢一
    • Journal Title

      化学と生物 47

      Pages: 390-396

    • NAID

      10026435645

    • Related Report
      2009 Annual Research Report
  • [Journal Article] A new benzoxepin metabolite isolated from endophytic fungus Phomopsis sp.2009

    • Author(s)
      Y.Shiono, A.Nitto, K.Shimanuki, T.Koseki, T.Murayama, T.Miyakawa, J.Yoshida, K.Kimura
    • Journal Title

      J.Antibiotics 62

      Pages: 533-535

    • NAID

      10030636414

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] サワラの球果由来PisiferdiolとCa^<2+>シグナル伝達に関わる遺伝子変異酵母株に対する生育円活性2011

    • Author(s)
      油井信弘、宮川都吉、大西素子、木村賢一
    • Organizer
      第55回香料・テルペンおよび精油化学に関する討論会
    • Place of Presentation
      つくば
    • Related Report
      2011 Final Research Report
  • [Presentation] 山菜のウドとセリ科野菜に含まれるGSK-3β阻害物質falcarindiolの糖尿病に対する効果2010

    • Author(s)
      吉田潤、諏訪紗央里、伊藤芳明、宮川都吉、木村賢一
    • Organizer
      第15回日本フードファクター学会
    • Place of Presentation
      仙台市民会館(宮城)
    • Year and Date
      2010-10-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] PP2C活性化物質PisiferdiolのCa^<2+>シグナル伝達に関わる遺伝子変異酵母に対する作用メカニズムの解析2010

    • Author(s)
      油井信弘、宮川都吉、大西素子、木村賢一
    • Organizer
      第5回日本ケミカルバイオロジー学会
    • Place of Presentation
      慶応大学(神奈川)
    • Year and Date
      2010-05-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Chemical investigation on biological active compounds from endophytic fungi2010

    • Author(s)
      Y.Shiono, K.Kimura
    • Organizer
      日本農芸化学会2010年度(平成22年度)大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] 山菜のウドとセリ科野菜に含まれるGSK-3β阻害物質falcarindiolの糖尿病に対する効果2010

    • Author(s)
      吉田潤、諏訪紗央里、伊藤芳明、宮川都吉、木村賢一
    • Organizer
      第15回日本フードファクター学会
    • Place of Presentation
      仙台
    • Related Report
      2011 Final Research Report
  • [Presentation] PP2C活性化物質PisiferdiolのCa^<2+>シグナル伝達に関わる遺伝子変異酵母に対する作用メカニズムの解析2010

    • Author(s)
      油井信弘、宮川都吉、大西素子、木村賢一
    • Organizer
      第5回日本ケミカルバイオロジー学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report
  • [Presentation] Chemical investigation on biological active compounds from endophytic fungi2010

    • Author(s)
      Shiono, Y. and Kimura, K.
    • Organizer
      2010年度日本農芸化学会シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 遺伝子変異酵母を用いた新たなバイオプローブの探索とその分子標的研究2009

    • Author(s)
      木村賢一
    • Organizer
      いわて海洋バイオテクノロジ-研究会研究交流技術セミナー
    • Place of Presentation
      釜石
    • Year and Date
      2009-08-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 遺伝子変異酵母を用いた新たなバイオプローブの探索とその分子標的研究2009

    • Author(s)
      木村賢一
    • Organizer
      いわて海洋バイオテクノロジー研究会
    • Place of Presentation
      釜石マリンバイオテクノロジー研究所(岩手県)
    • Year and Date
      2009-08-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] Ca^<2+>シグナル伝達に関わる遺伝子変異酵母の阻害剤に対する表現型と標的分子との関連性2009

    • Author(s)
      吉田潤、宮川都吉、木村賢一
    • Organizer
      日本ケミカルバイオロジー学会第4回年会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] サワラの球果由来PisiferdiolとCa^<2+>シグナル伝達に関わる遺伝子変異酵母株に対する生育円活性

    • Author(s)
      油井信弘、宮川都吉、大西素子、木村賢一
    • Organizer
      第55回香料・テルペンおよび精油化学に関する討論会
    • Place of Presentation
      筑波大学
    • Related Report
      2011 Annual Research Report
  • [Book] 16 Endophytic microorganisms as a source of bioactive compounds, Bioactive Compounds from Natural sources (2nd Edition), Edited by Corrado Tringali, Edited by Corrado Tringali2012

    • Author(s)
      Shiono, Y. and Kimura, K.
    • Publisher
      Taylor & Francis
    • Related Report
      2011 Final Research Report
  • [Book] BIOACTIVE COMPOUNDS from NATURAL SOURCES (Second Edition) Edited by Corrado Tringali, Chapter 16. Endophytic microorganisms as a source of bioactive compounds2012

    • Author(s)
      Shiono, Y., Kimura, K.
    • Publisher
      Taylor & Francis
    • Related Report
      2011 Annual Research Report
  • [Remarks] 岩手大学(木村賢一)

    • URL

      http://univdb.iwate-u.ac.jp/openmain.jsp

    • Related Report
      2011 Final Research Report
  • [Remarks] 山形大学(塩野義人)

    • URL

      http://www.tr.yamagata-u.ac.jp/kyouindata.html

    • Related Report
      2011 Final Research Report
  • [Remarks] 研究代表者

    • URL

      http://univdb.iwate-u.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks] 研究分担者

    • URL

      http://www.tr.yamagata-u.ac.jp/kyouindata.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://univdb.iwate-u.ac.jp/openmain.jsp

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.tr.yamagata-u.ac.jp/kyouindata.html

    • Related Report
      2010 Annual Research Report
  • [Remarks] 岩手大学農学部ホームページ

    • URL

      http://news7al.atm.iwate-u.ac.jp/

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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