Studies on molecular targets of new biological compounds having activity against the mutant yeast involving calcium signaling
Project/Area Number |
21580108
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Iwate University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SHIONO Yoshihito 山形大学, 農学部, 准教授 (80361278)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | Saccharomyces cerevisiae (zds1Δerg3Δpdr1/3Δ) / Ca^<2+>-signaling / bemzophomopsin A / 9-(Methylsulfinyl) hexyl isothocyanate / falcarindiol / pisiferdiol / GSK-3β / calcineurin / Saccharomyces cerevisiae / Ca^<2+>-signal transduction / phenotype / eremoxylarin A / anthracobic acid A / benzophomopsin A / isothiocyanate / Eremoxylarine / Anthracobic acid / Benzophomopsin A |
Research Abstract |
A new compound, bemzophomopsin restored the growth activity of the yeast mutant strain (zds1Δ erg3Δ pdr1/3Δ) at 1.25μg/disc and has no inhibition activity against calcineurin at 100 μM. 9-(Methylsulfinyl) hexyl isothocyanate inhibited GSK-3β with ATP competitive manner on Ki = 10.5μM and falcarindiol inhibited GSK-3β with ATP non-competitive manner on Ki = 86.9μM and the peptide substrate competitive manner on K_i=46.5μM. Pisiferdiol decreased the expression of calcineurin in the mutant yeast.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] A new benzoxepin metabolite isolated from endophytic fungus Phomopsis sp.2009
Author(s)
Shiono, Y., Nitto, A., Shimanuki, K., Koseki, T., Murayama, T., Miyakawa, T., Yoshida, J. and Kimura, K.
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Journal Title
J. Antibiot
Volume: 62
Pages: 533-535
NAID
Related Report
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