Evaluation of cDNA synthesis and RNA amplification with highly efficient reverse transcriptases and expansion of their use
| Project/Area Number |
21580110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 酵素利用学 / ウイルス / 核酸 / 酵素 / 酵素反応 / バイオテクノロジー |
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| Research Abstract |
We generated highly thermostable reverse transcriptases from Moloney murine leukemia virus and avian myeloblastosis virus(E286R/E302K/L435R/D524A and V238R/L388R/D450A, respectively) by site-directed mutagenesis. It is suggested that introduction of positive charges into reverse transcriptases at positions that have been implicated in the interaction with T/P is effective to stabilize reverse transcriptases.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Comparison of the Thermal Stabilities of the .ALPHA..BETA. Heterodimer and the .ALPHA. Subunit of Avian Myeloblastosis Virus Reverse Transcriptase2011
Author(s)
Konishi, A., Nemoto, D., Yasukawa, K., and Inouye, K.
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 75
Issue: 8
Pages: 1618-1620
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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