Functional analysys of cGMP/NO as signal transducers in plant cells

• Project/Area Number: 21580111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kobe University
• Principal Investigator: YAMAGATA Hiroshi 神戸大学, 大学院・農学研究科, 教授 (00159203)
• Co-Investigator(Kenkyū-buntansha): KANAMARU Kengo 神戸大学, 大学院・農学研究科, 准教授 (90260025)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 情報伝達 / cGMP / 一酸化窒素 / シグナル伝達 / 植物 / 遺伝子発現調節 / シロイヌナズナ

Research Abstract

The role of cGMP and NO in plant cells is not well understand. The mechanisms of signal transduction of cGMP/NO at the level of gene expression were studied. cGMP/NO responsible elements in the promoters of cGMP/NO regulated genes in Arabidopsis were determined. cGMP-responsible element was different from the NO-responsible element in both promoters, suggesting that cGMP and NO signal transduction pathway are different from each other. Unit I sequence in the promoter of soybean chalcone synthase 8 gene was shown to be cGMP-dependent cis-element.

Research Products

• [Journal Article] Characterization of three homoeologous cDNAs encoding chloroplast-targeted aminolevulinic acid dehydratase in common wheat (2011)
  • Author(s): Takenouchi, Y., 他
  • Journal Title: J.Integr.Plant Biol. Volume: 53 Issue: 12 Pages: 942-950
  • DOI: 10.1111/j.1744-7909.2011.01083.x
  • Peer Reviewed
• [Journal Article] 大豆フラボノイド合成系酵素遺伝子発現を調節するcGMP/NOシグナル伝達機構の解析 (2010)
  • Author(s): 吹田憲治、小原達矢、矢野俊介、豊島美咲、山形裕士
  • Journal Title: 大豆たん白質研究 Volume: Vol.13 Pages: 55-61
• [Journal Article] Functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme (2010)
  • Author(s): Nakagawa, M., Ueyama, M., Tsuruta, H., Uno, T., Kanamaru, K., Mikami, B., and Yamagata, H.
  • Journal Title: J. Biol. Chem. Volume: 285 Pages: 29797-29807
  • NAID: 120003220000
  • Peer Reviewed
• [Journal Article] Functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme (2010)
  • Author(s): Nakagawa, M., 他
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 29797-29807
  • Peer Reviewed
• [Journal Article] 大豆フラボノイド合成系酵素遺伝子発現を調節するcGMP/NOシグナル伝達機構の解析 (2010)
  • Author(s): 吹田憲治, 他
  • Journal Title: 大豆たん白質研究 Volume: 13 Pages: 55-61
• [Journal Article] Further Evaluation of the Localization and Functionality of Hemagglutinin Epitope-and Fluorescent Protein-Tagged AtMinD1 in Arabidopsis thaliana. (2009)
  • Author(s): Fujiwara, M., Dongliang, Li., Kazama, Y., Abe, T., Uno, T., Yamagata, H., Kanamaru, K., and Itoh, R.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 73(7) Pages: 1693-1697
  • NAID: 10027544042
• [Journal Article] Cyclic GMP acts as a common regulator for the transcriptional activation of flavonoid biosynthetic pathway in soybean (2009)
  • Author(s): Suita, K., Kiryu, T., Sawada, M., Mitsui, M., Nakagawa, M., Kanamaru, K., and Yamagata, H.
  • Journal Title: Planta Volume: 229 Pages: 403-413
  • NAID: 120001478935
  • Peer Reviewed
• [Journal Article] 作物の抵抗性誘導 (2009)
  • Author(s): 渡辺和彦、杉本琢真、大塩哲視、吹田憲治、山形裕士
  • Journal Title: 一酸化窒素の病害抵抗性への関与 Volume: 第2巻, 6 Pages: 58-68
• [Journal Article] Cyclic GMP acts as a common regulator for the transcriptional activation of flavonoid biosynthetic pathway in soybean. (2009)
  • Author(s): Suita, K., 他
  • Journal Title: Planta 229 Pages: 403-413
  • Peer Reviewed
• [Journal Article] Further Evaluation of the Localization and Functionality of Hemagglutinin Epitope- and Fluorescent Protein-Tagged AtMinD1 in Arabidopsis thaliana (2009)
  • Author(s): Fujiwara, M., 他
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 1693-1697
  • Peer Reviewed
• [Journal Article] 作物の抵抗性誘導.一酸化窒素の病害抵抗性への関与 (2009)
  • Author(s): 渡辺和彦
  • Journal Title: 作物栄養V 2 Pages: 58-68
• [Journal Article] (2009)
  • Author(s): 渡辺和彦
  • Journal Title: 最新農業技術 土壌施肥((社)農山漁村文化協会) Pages: 83-93
• [Presentation] Transcriptional control of fruit-specific expression of cucumisin and its application for transgenic plants that accumulate useful proteins in the fruit (2011)
  • Author(s): Atsuko Furukawa, Shunsuke Yasuda, Shinya Okuyama, Emiko Take, Mamoru Furuichi, Rena Azuma, Noriyuki Shibamoto, Tomohide Uno, Kengo Kanamaru, and Hiroshi Yamagata
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference(SOL & ICuGI 2011)
  • Place of Presentation: Kobe Convention Center
  • Year and Date: 2011-11-30
• [Presentation] Transcriptional control of fruit-specific expression of cucumisin and its application for transgenic plants that accumulate useful proteins in the fruit (2011)
  • Author(s): Hiroshi Yamagata
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference
  • Place of Presentation: Kobe Convention Center(兵庫県)
  • Year and Date: 2011-11-30
• [Presentation] 色素体局在性脂肪酸合成律速酵素の発現と機能 (2011)
  • Author(s): 小島志織, 山本俊佑, 能勢琢也, 朴杓允, 宇野知秀, 山形裕士, 金丸研吾
  • Organizer: 日本農芸化学会2011年度大会
  • Year and Date: 2011-03-27
• [Presentation] 大豆フラボノイド合成系酵素遺伝子発現を調節するcGMP/NOシグナル伝達機構の解析 (2010)
  • Author(s): 山形裕士
  • Organizer: 第13回不二たん白研究財団報告会
  • Year and Date: 2010-06-01
• [Presentation] 葉緑体発達に必須なシロイヌナズナPPRの分子機能解析 (2009)
  • Author(s): 小島志織
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Remarks]
  • URL: http://www2.kobe-u.ac.jp/%7Eunotom/
• [Remarks]
  • URL: http://www2.kobe-u.ac.jp/%7Eunotom/
• [Remarks]
  • URL: http://www2.kobe-u.ac.jp/%7Eunotom/
• [Remarks]
  • URL: http://www2.kobe-u.ac.jp/%7Eunotom/