Clarification of molecular mechanism for active biosynthesis and abundant accumulation of ascorbic acid in acerola.

• Project/Area Number: 21580113
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Hiroshima University
• Principal Investigator: ESAKA Muneharu 広島大学, 大学院・生物圏科学研究科, 教授 (70151975)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アスコルビン酸 / アセロラ / 遺伝子組換え / 遺伝子発現 / 酸化ストレス / ストレス抵抗性植物 / 熱帯性植物 / ビタミンC / デヒドロアスコルビン酸 / モノデヒドロアスコルビン酸 / プロモーター / マンノースピロホスホリラーゼ

Research Abstract

Acerola(Malpighia glabra) is an exotic fruit cultivated primarily for its abundant ascorbate content, which is over 20-fold higher than ascorbate content in lemon. The molecular mechanisms that regulate the biosynthesis and accumulation of a large amount of ascorbic acid in acerola have been clarified in this study. Also, ascorbic acid biosynthesis-related genes from acerola have been manipulated to generate ascorbic acid-rich transgenic plants. The overexpression of acerola ascorbate biosynthetic enzyme genes resulted in two-to three-fold increase in the ascorbate content of the transgenic plants.

Research Products

• [Journal Article] Overexpression of the acerola(Malpighiaglabra) monodehy droascorbate reductase gene in transgenic tobacco plants resultsin increased ascorbate levels and enhanced tolerance to salt stress (2012)
  • Author(s): Eltelib H. A., Fujikawa Y., Esaka M.
  • Journal Title: South African Journal of Botany Volume: 78 Pages: 295-301
  • Peer Reviewed
• [Journal Article] Characterization of secretory phospholipase A2 with phospholipase A1activity in tobacco (2012)
  • Author(s): Fujikawa Y., Fujikawa R., Iijima N., Esaka M.
  • Journal Title: Nicotiana tabacum(L.). Lipids Volume: 47 Pages: 303-312
  • Peer Reviewed
• [Journal Article] Overexpression of the acerola (Malpighia glabra) monodehydroascorbate reductase gene in transgenic tobacco plants results in increased ascorbate levels and enhanced tolerance to salt stress (2012)
  • Author(s): Eltelib HA, Fujikawa Y, Esaka M.
  • Journal Title: South African Journal of Botany Volume: 78 Pages: 295-301
  • NAID: 120005308220
  • Peer Reviewed
• [Journal Article] Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola(Malpighia glabra) (2011)
  • Author(s): Eltelib H. A., Badejo A. A., Fujikawa Y., Esaka M.
  • Journal Title: J. Plant Physiol Volume: 168(6) Pages: 619-627
  • Peer Reviewed
• [Journal Article] Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola (Malpighia glabra) (2011)
  • Author(s): Eltelib HA, Badejo AA, Fujikawan Y, Esaka M
  • Journal Title: Journal of Plant Physiology Volume: 168(6) Pages: 619-627
  • Peer Reviewed
• [Journal Article] Light regulation of ascorbic acid biosynthesis in rice via light responsivecis-elements in genes encoding ascorbic acid biosynthetic enzymes (2010)
  • Author(s): Fukunaga, K., Fujikawa, Y., Esaka, M.
  • Journal Title: Biosci Biotechnol Biochem Volume: 74(4) Pages: 888-891
  • Peer Reviewed
• [Journal Article] Fruits are more sensitive to salinity thanleaves and stems in pepper plants(Capsicum annuum L.) (2010)
  • Author(s): Azuma, R., Ito, N., Nakayama, N., Suwa, R., Nguyen, N. T., Larrinaga-Mayoral, J. A., Esaka, M., Fujiyama, H., Saneoka, H.
  • Journal Title: Scientia Horticulturae Volume: 125(3) Pages: 171-178
  • Peer Reviewed
• [Journal Article] Gene expression of monodehydroascorbate reductase and dehydroascorbate reductase during fruit ripening and in response to environmental stresses in acerola(Malpighiaglabra). (2010)
  • Author(s): Eltelib HA, Badejo AA, Fujikawa Y, Esaka M.
  • Journal Title: J.Plant Physiol. Volume: 168 Pages: 619-627
  • Peer Reviewed
• [Journal Article] Light regulation of ascorbic acid biosynthesis in rice via light responsive cis-elements in genes encoding ascorbic acidbiosynthetic enzymes. (2010)
  • Author(s): Fukunaga K, Fujikawa Y, Esaka M
  • Journal Title: Biosci Biotechnol Biochem. Volume: 74 Pages: 888-891
  • NAID: 10027554378
  • Peer Reviewed
• [Journal Article] Fruits are more sensitive to salinity than leaves and stems in pepper plants(Capsicum annuum L.). (2010)
  • Author(s): Azuma R, Ito N, Nakayama N, Suwa R, Nguyen NT, Larrinaga-Mayoral JA, Esaka M, Fujiyama H, Saneoka H
  • Journal Title: Scientia Horticulturae Volume: 125 Pages: 171-178
  • Peer Reviewed
• [Journal Article] Light regulation of ascorbic acid biosynthesis in rice via light responsive cis-elements in genes encoding ascorbic acid biosynthetic enzymes (2010)
  • Author(s): Fukunaga K, Fujikawa Y, Esaka M
  • Journal Title: Biosci.Biotechnol.Biochem. (in press)
  • NAID: 10027554378
  • Peer Reviewed
• [Journal Article] Gene expression of ascorbic acid biosynthesis related enzymes of the Smirnoff-Wheeler pathway in acerola(Malpighia glabra) (2009)
  • Author(s): Badejo A. A., Fujikawa Y., Esaka, M.
  • Journal Title: J. Plant Physiol Volume: 166(6) Pages: 652-660
  • Peer Reviewed
• [Journal Article] Increase in ascorbate content of transgenic tobacco plants overexpressing acerola(Malpighia glabra) phosphomannomutase gene (2009)
  • Author(s): Badejo A. A., Eltelib, H. A. M., FukunagaK., Fujikawa Y., Esaka, M.
  • Journal Title: Plant Cell Physiol Volume: 50(2) Pages: 423-428
  • NAID: 10027338001
  • Peer Reviewed
• [Journal Article] Increase in ascorbate content of transgenic tobacco plants overexpressing the acerola (Malpighia glabra) phosphomannomutase gene (2009)
  • Author(s): Badejo AA, Eltelib HA, Fukunaga K, Fujikawa Y, Esaka M.
  • Journal Title: Plant Cell Physiol. 50 Pages: 423-428
  • NAID: 10027338001
  • Peer Reviewed
• [Journal Article] Gene expression of ascorbic acid biosynthesis related enzymes of Smirnoff-Wheeler pathway in acerola (Malpighia glabra) (2009)
  • Author(s): Badejo A.A, Fujikawa Y, Esaka M.
  • Journal Title: J.Plant Physiol. 166 Pages: 652-660
  • Peer Reviewed
• [Presentation] モリンガにおけるアスコルビン酸生合成酵素遺伝子の発現解析 (2012)
  • Author(s): 近藤隆之、藤川愉吉、上田晃弘、長岡俊徳、実岡寛文、Manuel Calcano, MiltonMartinez, Jose Martich, 江坂宗春
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: Kyoto
  • Year and Date: 2012-03-24
• [Presentation] モリンガにおけるアスコルビン酸生合成酵素遺伝子の発現解析 (2012)
  • Author(s): 近藤隆之、藤川愉吉、上田晃弘、長岡俊徳、実岡寛文、Manuel Calcano, Milton Martinez, Jose Martich, 江坂宗春
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] Higher expression of vitamin C biosynthesis genesleads to higher vitamin C contents inacerola(Malpighia glabra) compared toArabidopsis thaliana (2010)
  • Author(s): Badejo, A. A., Esaka, M.
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
• [Presentation] Expression of Vitamin C Biosynthesis Genes leads to higher Vitamin C Contents in Acerola(Malpighia glabra)compared to Arabidopsis thaliana. (2010)
  • Author(s): Badejo AA, Esaka M.
  • Organizer: 21ST INTERNATIONAL CONFERENCE ON ARABIDOPSIS RESEARCH Yokohama
  • Place of Presentation: Yokohama, Japan
• [Presentation] トマトにおけるミオイノシトールオキシゲナーゼの発現解析 (2009)
  • Author(s): 坂本真吾, 渡辺元隆, 村野亜沙子, 藤川愉吉, 江坂宗春
  • Organizer: 日本農芸化学会関西・中四国・西日本支部、日本栄養・食料学会九州・沖縄支部、日本食品科学工学会西日本支部、2009年度合同沖縄大会
  • Place of Presentation: 琉球大学千原キャンパス,沖縄県中頭郡西原町
  • Year and Date: 2009-10-31
• [Presentation] Gene Expression ofVitamin C Biosynthesis and Metabolism Related Enzymes in Exotic Fruit-Acerola (2009)
  • Author(s): Hani A. Eltelib, Adebanjo A. Badejo, 藤川愉吉, 江坂宗春
  • Organizer: 日本農芸化学会関西・中四国・西日本支部、日本栄養・食料学会九州・沖縄支部、日本食品科学工学会西日本支部、2009年度合同沖縄大会
  • Place of Presentation: 琉球大学千原キャンパス,沖縄県中頭郡西原町
  • Year and Date: 2009-10-31
• [Presentation] トマトにおけるミオイノシトールオキシゲナーゼの発現解析 (2009)
  • Author(s): 坂本真吾, 渡辺元隆, 村野亜沙子, 藤川愉吉, 江坂宗春
  • Organizer: 日本農芸化学会関西・中四国・西日本支部、日本栄養・食料学会九州・沖縄支部、日本食品科学工学会西日本支部、2009年度合同沖縄大会
  • Place of Presentation: 琉球大学千原キャンパス(沖縄県中頭郡西原町)
  • Year and Date: 2009-10-31
• [Presentation] Gene Expression of Vitamin C Biosynthesis and Metabolism Related Enzymes in Exotic Fruit-Acerola (2009)
  • Author(s): Hani A. Eltelib, Adebanjo A. Badejo, 藤川愉吉, 江坂宗春
  • Organizer: 日本農芸化学会関西・中四国・西日本支部、日本栄養・食料学会九州・沖縄支部、日本食品科学工学会西日本支部、2009年度合同沖縄大会
  • Place of Presentation: 琉球大学千原キャンパス(沖縄県中頭郡西原町)
  • Year and Date: 2009-10-31
• [Book] 遺伝子組換え植物・作物,生命・食・環境のサイエンス (2011)
  • Author(s): 江坂宗春
  • Publisher: 江坂宗春監修,共立出版
• [Book] 生命・食・環境のサイエンス(江坂宗春監修) (2011)
  • Author(s): 江坂宗春
  • Total Pages: 220
  • Publisher: 共立出版
• [Book] Identificationof potential gene targets for theimprovement of ascorbate contents ofgenetically modified plants In Ascorbate-Glutathione Pathway and StressTolerance in Plants, Ed. Naser A. Anjum, Shahid Umar, Ming-Tsair Chan (2010)
  • Author(s): Badejo, A. A., Esaka, M.
  • Publisher: Springer, Dordrecht, Heidelberg, London, New York
• [Book] Identification of Potential Gene Targets for the Improvement of Ascorbate Contents of Genetically Modified Plants. (2010)
  • Author(s): Badejo AA, Esaka M.
  • Publisher: Springer Dordrecht Heidelberg, London New York
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/enzyme/
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  • URL: http://home.hiroshima-u.ac.jp/enzyme/esaka.html
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