Clarification of molecular mechanism for active biosynthesis and abundant accumulation of ascorbic acid in acerola.
Project/Area Number |
21580113
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Hiroshima University |
Principal Investigator |
ESAKA Muneharu 広島大学, 大学院・生物圏科学研究科, 教授 (70151975)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | アスコルビン酸 / アセロラ / 遺伝子組換え / 遺伝子発現 / 酸化ストレス / ストレス抵抗性植物 / 熱帯性植物 / ビタミンC / デヒドロアスコルビン酸 / モノデヒドロアスコルビン酸 / プロモーター / マンノースピロホスホリラーゼ |
Research Abstract |
Acerola(Malpighia glabra) is an exotic fruit cultivated primarily for its abundant ascorbate content, which is over 20-fold higher than ascorbate content in lemon. The molecular mechanisms that regulate the biosynthesis and accumulation of a large amount of ascorbic acid in acerola have been clarified in this study. Also, ascorbic acid biosynthesis-related genes from acerola have been manipulated to generate ascorbic acid-rich transgenic plants. The overexpression of acerola ascorbate biosynthetic enzyme genes resulted in two-to three-fold increase in the ascorbate content of the transgenic plants.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Fruits are more sensitive to salinity thanleaves and stems in pepper plants(Capsicum annuum L.)2010
Author(s)
Azuma, R., Ito, N., Nakayama, N., Suwa, R., Nguyen, N. T., Larrinaga-Mayoral, J. A., Esaka, M., Fujiyama, H., Saneoka, H.
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Journal Title
Scientia Horticulturae
Volume: 125(3)
Pages: 171-178
Related Report
Peer Reviewed
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[Presentation] トマトにおけるミオイノシトールオキシゲナーゼの発現解析2009
Author(s)
坂本真吾, 渡辺元隆, 村野亜沙子, 藤川愉吉, 江坂宗春
Organizer
日本農芸化学会関西・中四国・西日本支部、日本栄養・食料学会九州・沖縄支部、日本食品科学工学会西日本支部、2009年度合同沖縄大会
Place of Presentation
琉球大学千原キャンパス,沖縄県中頭郡西原町
Year and Date
2009-10-31
Related Report
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[Presentation] トマトにおけるミオイノシトールオキシゲナーゼの発現解析2009
Author(s)
坂本真吾, 渡辺元隆, 村野亜沙子, 藤川愉吉, 江坂宗春
Organizer
日本農芸化学会関西・中四国・西日本支部、日本栄養・食料学会九州・沖縄支部、日本食品科学工学会西日本支部、2009年度合同沖縄大会
Place of Presentation
琉球大学千原キャンパス(沖縄県中頭郡西原町)
Year and Date
2009-10-31
Related Report
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[Book] Identificationof potential gene targets for theimprovement of ascorbate contents ofgenetically modified plants In Ascorbate-Glutathione Pathway and StressTolerance in Plants, Ed. Naser A. Anjum, Shahid Umar, Ming-Tsair Chan2010
Author(s)
Badejo, A. A., Esaka, M.
Publisher
Springer, Dordrecht, Heidelberg, London, New York
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