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I dentification and characterization of the genes involved in generation or quenching of reactive oxygen species

Research Project

Project/Area Number 21580115
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionYokohama City University

Principal Investigator

FUJII Michihiko  横浜市立大学, 生命ナノシステム科学研究科, 准教授 (80285155)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords代謝生理 / 活性酸素
Research Abstract

Oxygen is essential for aerobic organisms, but generates reactive oxygen species(ROS), which can cause cellular dysfunction that would accelerate aging and diseases. We have studied the genes involved in the generation or the quenching of ROS with use of the nematode Caenorhabditis elegans as a model organism. In this study, we analyzed the expression patterns of OXY-5 and RAD-8 in C. elegans, and identified genes that, when down regulated, cause an increased sensitivity to ROS by systematic screening of the genes located on linkage group I.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (43 results)

All 2012 2011 2010 2009

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (26 results) Book (1 results)

  • [Journal Article] Systematic screen for genes involved in the regulation of oxidative stress in the nematode Caenorhabditis elegans2012

    • Author(s)
      S. Ueno, K. Yasutake, D. Tohyama, T. Fujimori, D. Ayusawa, M. Fujii
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 420 Issue: 3 Pages: 552-557

    • DOI

      10.1016/j.bbrc.2012.03.032

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sublethal Doses of Surfactants Induce Premature Senescence in Normal Human Skin Cells2011

    • Author(s)
      Y. Yamakami, K. Miki, M. Endoh, R. Yonekura, R. Ukekawa, Y. Kobayashi, M. Fujii, D. Ayusawa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 7 Pages: 1395-1398

    • DOI

      10.1271/bbb.110179

    • NAID

      10029329611

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] N-terminal short fragment of TUP1 confers resistance to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae2011

    • Author(s)
      S. Takayama, M. Fujii, Y. Nakagawa, K. Miki, D. Ayusawa
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 411 Issue: 1 Pages: 25-31

    • DOI

      10.1016/j.bbrc.2011.06.064

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A mutation in a mitochondrial dehydrogenase/reductase gene causes an increased sensitivity to oxidative stress and mitochondrial defects in the nematode Caenorhabditis elegans2011

    • Author(s)
      M. Fujii, K. Yasuda, P. S. Hartman, D. Ayusawa, N. Ishii
    • Journal Title

      Genes Cells

      Volume: 16 Issue: 10 Pages: 1022-1034

    • DOI

      10.1111/j.1365-2443.2011.01547.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutation in a mitochondrial ribosomal protein causes increased sensitivity to oxygen with decreased longevity in the nematode Caenorhabditis elegans2011

    • Author(s)
      Fujii M, Shikatani K, Ogura K, Goshima Y, Ayusawa D.
    • Journal Title

      Genes Cells

      Volume: 16(1) Issue: 1 Pages: 69-79

    • DOI

      10.1111/j.1365-2443.2010.01469.x

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutation in a mitochondrial ribosomal protein causes increased sensitivity to oxygen with decreased longevity in the nematode Caenorhabditis elegans2011

    • Author(s)
      M.Fujii, K.Shikatani, K.Ogura, Y.Goshima, D.Ayusawa
    • Journal Title

      Grenes Cells

      Volume: 16 Pages: 69-79

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 5-bromodeoxyuridine induces transcription of repressed genes with disruption of nucleosome positioning2010

    • Author(s)
      K. Miki, M. Shimizu, M. Fujii, S. Takayama, M. N. Hossain, D. Ayusawa
    • Journal Title

      Febs J

      Volume: 277 Issue: 21 Pages: 4539-4548

    • DOI

      10.1111/j.1742-4658.2010.07868.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of genes that affect sensitivity to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae2010

    • Author(s)
      M. Fujii, K. Miki, S. Takayama, D. Ayusawa
    • Journal Title

      Mol Genet Genomics

      Volume: 283 Issue: 5 Pages: 461-468

    • DOI

      10.1007/s00438-010-0535-6

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 5-bromodeoxyuridine induces transcription of repressed genes with disruption of nucleosome positioning2010

    • Author(s)
      Milki, K., Shimizu, M., Fujii, M., Takayama, S., Hossain, M.N., Ayusawa, D.
    • Journal Title

      Febs J.

      Volume: 277 Pages: 4539-48

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of genes that affect sensitivity to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae2010

    • Author(s)
      Fujii, M., Milki, K., Takayama, S., Ayusawa, D.
    • Journal Title

      Mot Genet Genomics

      Volume: 283 Pages: 461-8

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterozygous Disruption of the DNA Topoisomerase I Gene Confers Cellular Resistance to Camptothecin in Human Cells2009

    • Author(s)
      E. Toyoda, A. Kurosawa, M. Fujii, N. Adachi
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 32 Issue: 4 Pages: 724-727

    • DOI

      10.1248/bpb.32.724

    • NAID

      110007160669

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] [FeFe]-hydrogenase-like gene is involved in the regulation of sensitivity to oxygen in yeast and nematode2009

    • Author(s)
      M. Fujii, N. Adachi, K. Shikatani, D. Ayusawa
    • Journal Title

      Genes Cells

      Volume: 14 Issue: 4 Pages: 457-468

    • DOI

      10.1111/j.1365-2443.2009.01282.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Coordinate expression of the human pregnancy-specific glycoprotein gene family during induced and replicative senescence2009

    • Author(s)
      M. Endoh, Y. Kobayashi, Y. Yamakami, R. Yonekura, M. Fujii, D. Ayusawa
    • Journal Title

      Biogerontology

      Volume: 10 Issue: 2 Pages: 213-221

    • DOI

      10.1007/s10522-008-9173-3

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heterozygous disruption of the DNA topoisomerase I gene confers cellular resistance to camptothecin in human cells2009

    • Author(s)
      Toyoda, E., Kurosawa, A., Fujii, M., Adachi, N.
    • Journal Title

      Biol Pharm Bull. 32

      Pages: 724-727

    • NAID

      110007160669

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] [FeFe]-hydrogenase-like gene is involved in the regulation of sensitivity to oxygen in yeast and nematode2009

    • Author(s)
      Fujii, M., Adachi, N., Shikatani, K., Ayusawa, D.
    • Journal Title

      Genes Cells 14

      Pages: 457-468

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] (2009)Coordinate expression of the human pregnancy-specific glycoprotein gene family during induced and replicative senescence2009

    • Author(s)
      Endoh, M., Kobayashi, Y., Yamakami, Y, Yonekura, R., Fujii, M., Ayusawa, D.
    • Journal Title

      Biogerontology 10

      Pages: 213-221

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] アマラキ(Emblica officinalis)の不死化ヒト表皮角化細胞に対する生理作用2012

    • Author(s)
      杉本量平、三木健輔、山上義巳、Mohammad Nazir Hossain、山下律郎、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-24
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト老化細胞における核内構造の変化2012

    • Author(s)
      新井留実、谷脇歩美、三木健輔、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-24
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト毛乳頭細胞におけるアーユルヴェーダハーブの生理作用2012

    • Author(s)
      山口葉子、Mohammad N Hossain、三木健輔、山下律郎、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-24
    • Related Report
      2011 Final Research Report
  • [Presentation] アーユルヴェーダハーブによるヒト線維芽細胞の増殖効果2012

    • Author(s)
      三木健輔、三田充真、藤井道彦、Mohammad N Hossain、山下律郎、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Gタンパク質γサブユニットGNG11の細胞老化における役割2012

    • Author(s)
      工藤生、伊藤陽平、古賀慎太郎、Mohammad N Hossain、三木健輔、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 出芽酵母Saccharomyces cerevisiaeを用いた活性酸素抵抗性遺伝子の単離と解析2011

    • Author(s)
      溝渕翔吾, 藤井道彦, 鮎沢大
    • Organizer
      分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 出芽酵母Saccharomyces cerevisiaeにおける5-ブロモデオキシウリジンの作用機構の解析2011

    • Author(s)
      川口竜、中川優、藤井道彦、鮎沢大
    • Organizer
      分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気パルス法を用いた接着細胞へのin situ遺伝子導入2011

    • Author(s)
      三木健輔、山本祐介、早川清、早川靖彦、藤井道彦、鮎沢大
    • Organizer
      分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 出芽酵母Saccharomyces cerevisiaeを用いた活性酸素抵抗性遺伝子の単離と解析2011

    • Author(s)
      溝渕翔吾, 藤井道彦, 鮎沢大
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 準致死量の界面活性剤によるヒト正常細胞の未成熟老化の誘導機構2011

    • Author(s)
      山上義巳、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-24
    • Related Report
      2011 Final Research Report
  • [Presentation] (線虫(Caenorhabditis elegans)における高濃度酸素感受性遺伝子の探索2010

    • Author(s)
      上野峻介、藤井道彦、鮎沢大
    • Organizer
      分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Gタンパク質γサブユニットGNG11の細胞老化における役割2010

    • Author(s)
      工藤生, 伊藤陽平, 古賀慎太郎, Mohammad N HOSSAIN, 三木健輔, 藤井道彦, 鮎沢大
    • Organizer
      分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気パルス法を用いた遺伝子導入効率の最適化2010

    • Author(s)
      三木健輔、山本裕介、早川清、早川靖彦、藤井道彦、鮎沢大
    • Organizer
      分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 線虫(Caenorhabditis elegans)における高濃度酸素感受性遺伝子の探索2010

    • Author(s)
      上野峻介、藤井道彦、鮎沢大
    • Organizer
      分子生物学会2010年度大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 線虫C. elegansの酸化ストレス高感受性変異体oxy-4の解析2010

    • Author(s)
      藤井道彦、鹿谷一樹、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 出芽酵母Saccharomyces cerevisiaeにおける5.ブロモデオキシウリジン耐性機構の解析2010

    • Author(s)
      中川優, 三木健輔, 藤井道彦, 鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 界面活性剤の細胞毒性2010

    • Author(s)
      山上義巳, 藤井道彦, 鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 表皮角化細胞の増殖を促進するアマラキ(Emblica officinalis)含有成分の同定2010

    • Author(s)
      杉本量平、三木健輔、山上義巳、Mohammad N Hossain、山下律郎、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] メラノサイト機能へ影響を及ぼすアーユルヴェーダハーブの探索2010

    • Author(s)
      三木健輔、勝又三映子、Mohammad N HOSSAIN、山下律郎、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] がん細胞特異的な増殖阻害活性を示すアーユルヴェーダ植物の探索2010

    • Author(s)
      根本英比古、山上義巳、三木健輔、Mohammad N Hossain、山下律郎、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 高濃度の塩化ナトリウムによる細胞の老化誘導2010

    • Author(s)
      米倉隆造、松本祐子、小林裕介、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Gタンパク質γサブユニットGNG11の細胞老化における役割2010

    • Author(s)
      工藤生, 伊藤陽平, 古賀慎太郎, Mohammad N HOSSAIN, 三木健輔, 藤井道彦, 鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞老化におけるMAPKの役割2010

    • Author(s)
      小林裕介、蕭淑麗、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気パルス法を用いた遺伝子導入効率の最適化2010

    • Author(s)
      山本裕介、澁谷大輔、三木健輔、早川靖彦、藤井道彦、鮎沢大
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 線虫C.elegans の酸化ストレス高感受性変異体oxy-4の解析2010

    • Author(s)
      藤井道彦, 鹿谷一樹, 鮎沢大
    • Organizer
      2010年度農芸化学会大会
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] 線虫Caenorhabditis elegansを用いた酸化ストレス高感受性変異体oxy-4の解析2009

    • Author(s)
      藤井道彦、鹿谷一樹、鮎沢大
    • Organizer
      日本基礎老化学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-06-17
    • Related Report
      2011 Final Research Report
  • [Book] ワトソン 組換えDNAの分子生物学2009

    • Author(s)
      一部翻訳を担当
    • Total Pages
      477
    • Publisher
      丸善
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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