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Fundamental Research for the synthesis of biorecognition glycosides by enzymatic introduction of oligosaccharides from glycoproteins

Research Project

Project/Area Number 21580117
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionOsaka City University

Principal Investigator

ITO Kazuo  大阪市立大学, 大学院・理学研究科, 准教授 (20183171)

Co-Investigator(Renkei-kenkyūsha) MIYAHARA Ikuko  大阪市立大学, 大学院・理学研究科, 准教授 (40271176)
Research Collaborator HAMAGUCHI Tasuku  , 学生
TANIWAKI Satoshi  , 学生
UMEBAYASHI Hidetada  , 学生
OKA Yuki  , 学生
GOTO Mariko  , 学生
SHINOHARA Kanako  , 学生
RYU Hao  , 学生
KAIGUMI Takahiro  , 学生
TAKATANI Naoya  , 学生
TAKEUCHI Tomofumi  , 学生
YOSHIDA Tatsuya  , 学生
ISHII Maiko  , 学生
TAKAHASI Go  , 学生
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords糖鎖 / 生体認識 / 酵素 / 配糖体 / 生体材料
Research Abstract

Expression system and purification method of recombinant N-linked oligosaccharide-releasing enzyme was established. The recombinant enzyme released oligosaccharides from various glycoproteins. Furthermore, it transferred the released oligosaccharides to form potential bio-recognition glycosides. Analysis of mutant recombinant enzyme showed the functional area on the enzyme. Furthermore, Crystallization of the recombinant enzyme succeeded for X-ray structural analysis.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (55 results)

All 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (41 results) Book (2 results)

  • [Journal Article] Asparagine-linked oligosaccharide-releasing enzymes produced by basidiomycetes : Tasuku Hamaguchi2011

    • Author(s)
      Yukako Inoue, Tsukasa Ito, Kanako Shinohara, Satoshi Taniwaki, Nobuhiro Kaizumi, Hiromoto Hisada, Yoji Hata, Tipaporn Limpaseni, Piamsook Pongsawasdi, and Kazuo Ito
    • Journal Title

      Oyotositukagaku

      Volume: 1 Pages: 159-167

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of epicatechin glucosides by aυ-cyclodextrin glycosyltransferase : Pornpun Aramsangtienchai2011

    • Author(s)
      Warinthorn Chavasiri, Kazuo Ito and Piamsook Pongsawasdi
    • Journal Title

      Journal of Molecular Catalysis B : Enzymatic

      Volume: 73 Pages: 27-34

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and biochemical characterization of levansucrase from Bacillus licheniformis RN-01.Santhana Nakapong, Rath Pichyangkura2011

    • Author(s)
      Masaru Iizuka, Kazuo Ito and Piamsook Pongsawasdi.
    • Journal Title

      The 3^<rd> Biochemistry and Molecular Biology International Conference, Chiang Mai

      Pages: 6-8

    • Related Report
      2011 Final Research Report
  • [Journal Article] Synthesis of a novel prebiotic trisaccharide by a type Iτ-glucosidase from B. licheniformis strain TH4-2 : P. Nimpiboon2011

    • Author(s)
      Santhana Nakapong, Rath Pichyangura, Kazuo Ito, and Piamsook Pongsawasdi
    • Journal Title

      Process Biochem

      Volume: 46 Pages: 448-457

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Asparagine-linked oligosaccharide-releasing enzymes produced by basidiomycetes2011

    • Author(s)
      T.Hamaguchi, et al
    • Journal Title

      Oyotositukagaku

      Volume: 1 Pages: 159-167

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of epicatechin glucosides by αβ-cyclodextrin glycosyltransferase2011

    • Author(s)
      Chavasiri W., et al
    • Journal Title

      Journal of Molecular Catalysis B : Enzymatic

      Volume: 73 Pages: 27-34

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of a novel prebiotic trisaccharide by a type 1 α-glucosidase from B.heniformis strain TH4-22011

    • Author(s)
      Pitchanan Nimpiboon
    • Journal Title

      Process Biochemistry

      Volume: 46 Pages: 448-457

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of cellobiose-containing oligosaccharides by Intermolecular transglucosylation of cyclodextrin glycosyltransferase from Paenibacillus sp A11 : Wannapa Wongsangwattana2010

    • Author(s)
      Jarunee Kaulpiboon, Kazuo Ito, and Piamsook Pongsawasdi
    • Journal Title

      Process Biochemistry

      Volume: 45 Pages: 947-953

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification, Characterization and Molecular Cloning of a Novel Endo-υ-N-acetylglucosaminidase from the Basidiomycete, Flammulina velutipes2010

    • Author(s)
      Tasuku Hamaguchi, Tsukasa Ito, Yukako Inoue, Tipaporn, Limpaseni, Piamsook Pongsawasdi, and Kazuo Ito
    • Journal Title

      Glycobiology

      Volume: 1 Pages: 420-432

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Cellobiose-containing Oligosaccharides by Intermolecular Transglucosylation of Cyclodextrin Glycosyltransferase from Paenibacillus sp All2010

    • Author(s)
      Wannapa Wongsangwattana
    • Journal Title

      Process Biochemistry

      Volume: 45 Pages: 947-953

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification, Characterization and Molecular Cloning of a Novel Endo-β-N-acetylglucosaminidase from the Basidiomycete, Flammulina velutipes2010

    • Author(s)
      Tasuku Hamaguchi
    • Journal Title

      Glycobiology 20

      Pages: 420-432

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Cellobiose-containing Oligosaccharides by Intermolecular Transglucosylation of Cyclodextrin Glycosyltransferase from Paenibacillus sp All2010

    • Author(s)
      Wannapa Wongsangwattana
    • Journal Title

      Process Biochemistry 46(in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 担子菌Agaricus bisporus由来エンド-.-N-アセチルグルコサミニダーゼABの遺伝子クローニングに関する研究2012

    • Author(s)
      高橋剛生、谷脇聡、浜口祐、岡夕貴、Kuakarun Krusong、Jarunee Kaulpiboon、TipapornLimpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      2012年日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌Flammulina velutipes由来エンド-.-N-アセチルグルコサミニダーゼFVのプロセシング酵素の精製とその同定2012

    • Author(s)
      海住宜広、浜口祐、後藤眞璃子、KuakarunKrusong、Jarunee aulpiboon Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      2012年日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌Flammulina velutipes由来エンド-.-N-アセチルグルコサミニダーゼFV遺伝子の酵母での発現とX線結晶構造解析2012

    • Author(s)
      浜口祐、海住宜広、篠原かな子、宮原郁子、田中里佳、Kuakarn Kursong、Jarnee Kaurpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      2012年日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌Flammulina velutipes由来エンド-β-N-アセチルグルコサミニダーゼFV遺伝子の酵母での発現とX線結晶構造解析2012

    • Author(s)
      浜口祐
    • Organizer
      2012年日本農芸化学会大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 担子菌Flammulina velutipes由来エンド-β-N-アセチルグルコサミニダーゼFVのプロセシング酵素の精製とその同定2012

    • Author(s)
      海住宜広
    • Organizer
      2012年日本農芸化学会大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 担子菌Agaricus bisporus由来エンド-β-N-アセチルグルコサミニダーゼABの遺伝子クローニングに関する研究2012

    • Author(s)
      高橋剛生
    • Organizer
      2012年日本農芸化学会大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 担子菌Flammulina. velutipesにおけるアスパラギン結合型糖鎖遊離酵素のプロセシング酵素2011

    • Author(s)
      海住宜広、濱口祐、久田博元、秦洋二、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌由来エンド-.-N-アセチルグルコサミニダーゼFV遺伝子の酵母での発現と発現酵素の性質2011

    • Author(s)
      浜口祐、海住宜広、篠原かな子、Kuakarn Kursong、Jarnee Kaurpiborn、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌由来エンド-β-N-アセチルグルコサミニダーゼFV遺伝子の酵母での発現と発現酵素の性質2011

    • Author(s)
      浜口祐
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会議場(京都府)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 担子菌Flammulina.velutipesにおけるアスパラギン結合型糖鎖遊離酵素のプロセシング酵素2011

    • Author(s)
      海住宜広
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会議場(京都府)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 担子菌Flammulina. velutipesにおけるアスパラギン結合型糖鎖遊離酵素のプロセシング2011

    • Author(s)
      海住宜広、濱口祐、久田博元、秦洋二、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      第58回日本生化学会近畿支部例会
    • Place of Presentation
      大阪
    • Year and Date
      2011-05-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌Flammulina.velutipesにおけるアスパラギン結合型糖鎖遊離酵素のプロセシング2011

    • Author(s)
      海住宜広
    • Organizer
      第58回日本生化学会近畿支部例会
    • Place of Presentation
      関西医大(大阪府)
    • Year and Date
      2011-05-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular and biochemical characterization of levansucrase from Bacillus licheniformis RN-012011

    • Author(s)
      Nakapong, S., Pichyangkura, R., Iizuka, M., Ito, K., and Pongsawasdi, P.
    • Organizer
      The 3^<rd> Biochemistry and Molecular Biology International Conference
    • Place of Presentation
      Chiang Mai, Thailand
    • Year and Date
      2011-04-20
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 担子菌Agaricus bisporus由来エンド-.-N-アセチルグコサミニダーゼABの精製とその特異性2011

    • Author(s)
      谷脇聡、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      2011年日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌Agaricus bisporus由来エンド-β-N-アセチルグルコサミニダーゼABの精製とその特異性2011

    • Author(s)
      伊藤和央
    • Organizer
      2011年日本農芸化学会大会
    • Place of Presentation
      京都女子大(京都)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 担子菌Agaricus bisporus由来エンド-.-N-アセチルグルコサミニダーゼABの精製とその性質2010

    • Author(s)
      谷脇聡、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、PiamsookPongsawasdi、伊藤和央
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌Agaricus bisporus由来エンド-β-N-アセチルグルコサミニダーゼABの精製とその性質2010

    • Author(s)
      谷脇聡
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸ポートピアホール(神戸)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comparative study of basidiomycetes endoglycosidases acting on asparagine-linked oligosaccharides2010

    • Author(s)
      Satoshi Taniwaki, Tasuku Hamaguchi, Kuakarun Krusong, Jarunee Kaulpiboon, Tipaporn Limpaseni, Piamsook Pongsawasdi, and Kazuo Ito
    • Organizer
      The 2nd ACP Joint Seminar
    • Place of Presentation
      Khon Kaen, Thailand
    • Year and Date
      2010-11-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Mutation at subsite+2 asparagine residue of levansucrase caused the change in product size of levan2010

    • Author(s)
      Santhana Nakapong, Rath Pichyangkura, Masaru Iizuka, Kazuo Ito and Piamsook Pongsawasdi
    • Organizer
      The 2nd ACP Joint Seminar
    • Place of Presentation
      Khon Kaen, Thailand
    • Year and Date
      2010-11-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of amylomaltase from Thermus filiformis2010

    • Author(s)
      Kuakarun Krusong, Piriya Kaewpathomsri, Jarunee Kaulpiboon, Kazuo Ito, Shuichiro Murakami, and Piamsook Pongsawasdi
    • Organizer
      The 2nd ACP Joint Seminar
    • Place of Presentation
      Khon Kaen, Thailand
    • Year and Date
      2010-11-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of transglycosylation products of recombinant cyclodextringlycosyltransferase from Paenibacillus sp. BT012010

    • Author(s)
      Prapai Hongsa, Kazuo Ito and Tipaporn Limpaseni
    • Organizer
      The 2nd ACP Joint Seminar
    • Place of Presentation
      Khon Kaen, Thailand
    • Year and Date
      2010-11-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Comparative study of basidiomycetes endoglycosidases acting on asparagine-linked oligosaccharides2010

    • Author(s)
      伊藤和央
    • Organizer
      The 2^<nd> ACP Joint Seminar
    • Place of Presentation
      Khon Kaen, (Thailand)
    • Year and Date
      2010-11-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mutation at subsite+2 asparagine residue of levansucrase caused the change in product size of levan2010

    • Author(s)
      Piamsook Pongsawasdi
    • Organizer
      The 2^<nd> ACP Joint Seminar
    • Place of Presentation
      Khon Kaen, (Thailand)
    • Year and Date
      2010-11-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characterization of amylomaltase from Thermus filiformis2010

    • Author(s)
      Kuakarun Krusong
    • Organizer
      The 2^<nd> ACP Joint Seminar
    • Place of Presentation
      Khon Kaen, (Thailand)
    • Year and Date
      2010-11-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characterization of transglycosylation products of recombinant cyclodextringlycosyltransferase from Paenibacillus sp.BT012010

    • Author(s)
      Tipaporn Limpaseni
    • Organizer
      The 2^<nd> ACP Joint Seminar
    • Place of Presentation
      Khon Kaen, (Thailand)
    • Year and Date
      2010-11-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 担子菌Agaricus bisporusの産生する糖タンパク質糖鎖遊離酵素の精製とその特異性2010

    • Author(s)
      谷脇聡
    • Organizer
      2010年度日本農芸化学会関西支部大会
    • Place of Presentation
      近畿大学(奈良)
    • Year and Date
      2010-10-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 担子菌Agaricus bisporusの産生する糖タンパク質糖鎖遊離酵素の精製とその特異性2010

    • Author(s)
      谷脇聡、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      2010年度日本農芸化学会関西支部大会
    • Place of Presentation
      奈良
    • Year and Date
      2010-10-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌の産生するアスパラギン結合型糖鎖遊離酵素系2010

    • Author(s)
      濱口祐、井上裕香子、伊藤司、篠原かな子、谷脇聡、海住宜広、久田博元、秦洋二、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      第18回糖質関連酵素化学シンポジウム
    • Place of Presentation
      静岡
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌の産生するアスパラギン結合型糖鎖遊離酵素系2010

    • Author(s)
      伊藤和央
    • Organizer
      第18回糖質関連酵素化学シンポジウム
    • Place of Presentation
      静岡コンベンションセンター(静岡)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 担子菌Agaricus bisporus由来エンド-.-N-アセチルグルコサミニダーゼABの精製とその性質2010

    • Author(s)
      谷脇聡、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      第57回日本生化学会近畿支部例会
    • Place of Presentation
      奈良
    • Year and Date
      2010-05-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌Agaricus bisporus由来エンド-β-N-アセチルグルコサミニダーゼABの精製とその性質2010

    • Author(s)
      谷脇聡
    • Organizer
      第57回日本生化学会近畿支部例会
    • Place of Presentation
      奈良先端大(奈良)
    • Year and Date
      2010-05-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 担子菌由来ペプチドN-グリカナーゼの多様性2010

    • Author(s)
      篠原かな子、井上裕香子、小島美保子、濱口祐、伊藤和央
    • Organizer
      第11回関西グライコサイエンスフォーラム
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌由来ペプチドN-グリカナーゼの多様性2010

    • Author(s)
      篠原かな子
    • Organizer
      第11回関西グライコサイエンスフォーラム
    • Place of Presentation
      大阪市立大学(大阪)
    • Year and Date
      2010-05-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 担子菌由来新規ペプチドN-グリカナーゼFV遺伝子のクローニングに関する研究2010

    • Author(s)
      篠原かな子、井上裕香子、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
    • Organizer
      2010年日本農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌由来新規ペプチドN-グリカナーゼFV遺伝子のクローニングに関する研究2010

    • Author(s)
      篠原かな子
    • Organizer
      日本農芸化学会2010年大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 担子菌由来エンド-.-N-アセチルグルコサミニダーゼの発現系構築と発現酵素の性質2009

    • Author(s)
      濱口祐、谷脇聡、海住宜広、久田博元、秦洋二、伊藤和央
    • Organizer
      日本応用糖質科学会中国四国・近畿支部合同シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2009-11-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌由来エンド-β-N-アセチルグルコサミニダーゼの発現系構築と発現酵素の性質2009

    • Author(s)
      濱口祐
    • Organizer
      日本応用糖質科学会中国・四国・近畿支部合同シンポジウム
    • Place of Presentation
      岡山コンベンションセンター(岡山県)
    • Year and Date
      2009-11-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] 担子菌Flammulina velutipes由来ペプチドN-グリカナーゼの精製と遺伝子クローニング2009

    • Author(s)
      濱口祐、井上裕香子、篠原かな子、谷脇聡、伊藤和央
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌Flammulina velutipes由来ペプチドN-グリカナーゼの精製と遺伝子クローニング2009

    • Author(s)
      濱口祐
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 担子菌由来エンド-.-N-アセチルグルコサミニダーゼ遺伝子の発現系の構築2009

    • Author(s)
      濱口祐、谷脇聡、久田博元、泰洋二、伊藤和央
    • Organizer
      第56回日本生化学会近畿支部例会
    • Place of Presentation
      大阪
    • Year and Date
      2009-07-18
    • Related Report
      2011 Final Research Report
  • [Presentation] 担子菌由来エンド-β-N-アセチルグルコサミニダーゼ遺伝子の発現系の構築2009

    • Author(s)
      濱口祐
    • Organizer
      第56回日本生化学会近畿支部例会
    • Place of Presentation
      大阪医科大学(大阪府)
    • Year and Date
      2009-07-18
    • Related Report
      2009 Annual Research Report
  • [Book] Enzymatic Synthesis of Linear, Cyclic and Complex Type Oligosaccharides, Piamsook Pongsawasdiand, in Oligosaccharides : Sources, Properties and Applications Nicole S. Gordon ed2011

    • Author(s)
      Kazuo Ito
    • Publisher
      Nova Science Publishers
    • Related Report
      2011 Final Research Report
  • [Book] Enzymatic synthesis of linear, cyclic and complex type oligosaccharides in Oligosaccharides : Sources, Properties and Applications Nicole S.Gordon ed2011

    • Author(s)
      Piamsook Pongsawasdi, Kazuo Ito
    • Total Pages
      25
    • Publisher
      Nova Science Publishers
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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