| Project/Area Number |
21580117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Osaka City University |
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Principal Investigator |
ITO Kazuo 大阪市立大学, 大学院・理学研究科, 准教授 (20183171)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAHARA Ikuko 大阪市立大学, 大学院・理学研究科, 准教授 (40271176)
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| Research Collaborator |
HAMAGUCHI Tasuku , 学生
TANIWAKI Satoshi , 学生
UMEBAYASHI Hidetada , 学生
OKA Yuki , 学生
GOTO Mariko , 学生
SHINOHARA Kanako , 学生
RYU Hao , 学生
KAIGUMI Takahiro , 学生
TAKATANI Naoya , 学生
TAKEUCHI Tomofumi , 学生
YOSHIDA Tatsuya , 学生
ISHII Maiko , 学生
TAKAHASI Go , 学生
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 糖鎖 / 生体認識 / 酵素 / 配糖体 / 生体材料 |
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| Research Abstract |
Expression system and purification method of recombinant N-linked oligosaccharide-releasing enzyme was established. The recombinant enzyme released oligosaccharides from various glycoproteins. Furthermore, it transferred the released oligosaccharides to form potential bio-recognition glycosides. Analysis of mutant recombinant enzyme showed the functional area on the enzyme. Furthermore, Crystallization of the recombinant enzyme succeeded for X-ray structural analysis.
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