Molecular analysis of anaerobic benzene degradation

• Project/Area Number: 21580118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kitasato University
• Principal Investigator: KASAI Yuki 北里大学, 海洋バイオテクノロジー釜石研究所, 上級研究員 (20416572)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 酵素化学 / 嫌気性細菌 / Azoarcus / ベンゼン分解系遺伝子 / methyltransferase / タンパク質発現 / cDNA-substraction / SIGEX / 遺伝子 / 発現制御 / 微生物

Research Abstract

Intermediates formed during anaerobic benzene degradation by Azoarcus sp. DN11 was identified by gas chromatography/mass spectrometry. The data support initial methylation of benzene to toluene, followed by transformation to benzoate. Benzene-induced gene expression analysis suggested that there was a new methyltransferase gene near an anaerobic toluene degradation gene cluster. The overexpressed recombinant methyltransferase converted benzene to a metabolite that has the same retention time with toluene.

Research Products

• [Presentation] 微生物を利用した石油汚染環境浄化 (2011)
  • Author(s): 笠井由紀
  • Organizer: 日本生物工学会北日本支部シンポジウム
  • Place of Presentation: 岩手県民情報交流センター(岩手県)
  • Year and Date: 2011-12-03
• [Presentation] 微生物を利用した石油汚染環境浄化 (2011)
  • Author(s): 笠井由紀
  • Organizer: 日本生物工学会北日本支部シンポジウム
  • Place of Presentation: 岩手県民情報交流センター(岩手県)(招待講演)
  • Year and Date: 2011-12-03
• [Presentation] 嫌気ベンゼン分解菌Azoarcus sp. DN11株のベンゼン分解遺伝子解析 (2011)
  • Author(s): 笠井由紀
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-15
• [Presentation] 嫌気ベンゼン分解菌Azoarcus sp.DN11株のベンゼン分解遺伝子解析 (2011)
  • Author(s): 笠井由紀
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-15
• [Presentation] 嫌気ベンゼン分解菌Azoarcus sp. DN11株のゲノム解析 (2010)
  • Author(s): 笠井由紀
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 広島大学
  • Year and Date: 2010-11-22
• [Presentation] 嫌気ベンゼン分解菌Azoarcus sp.DN11株のゲノム解析 (2009)
  • Author(s): 笠井由紀
  • Organizer: 第25回日本微生物生態学会
  • Place of Presentation: 広島大学東広島キャンパス
  • Year and Date: 2009-11-22
• [Book] Microbial bioremediation of non-metals capter11 Molecular technologies for analysis of petroleum bioremediation (2011)
  • Author(s): 笠井由紀
  • Total Pages: 279
  • Publisher: Caister Academic Press
• [Book] Microbial bioremediation of non-metals capter11 Molecular technologies for a nalysis of petroleum bioremediation (2011)
  • Author(s): Yuki Kasai
  • Total Pages: 279
  • Publisher: Caister Academic Press
• [Patent(Industrial Property Rights)] ベンゼン分解菌の検出方法 (2011)
  • Inventor(s): 片山美津留、高畑陽、笠井由紀
  • Industrial Property Rights Holder: 大成建設株式会社
  • Industrial Property Number: 2011-168311
  • Filing Date: 2011-08-01