Establishment of high concentration Refolding of unstable protein
| Project/Area Number |
21580119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Sojo University |
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Principal Investigator |
MATSUMOTO Toshihiko (2010-2011) 崇城大学, 生物生命学部, 准教授 (60133568)
井本 泰治 (2009) Sojo University, 生物生命学部, 教授 (90038282)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Toshihiko 崇城大学, 生物生命学部, 准教授 (60133568)
YAMAURA Izumi 崇城大学, 生物生命学部, 教授 (30133565)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 再生 / フォルディング / 安定化剤 / 可溶化剤 / 不安定蛋白質 / タンパク質の再生 / リゾチーム / グリセリン / 透析再生法 / 高濃度生成法 / 不安定タンパク質 / 高濃度再生法 |
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| Research Abstract |
Earlier, we formally established an effective refolding procedure for a protein by gradient removal of a solubilizer such as urea. However, this procedure was less effective for unstable proteins. We developed here an excellent method to add protein stabilizer so as to get reasonable amounts of folded protein under the concentration of solubilizer where the unstable protein does not form aggregate. We examined many stabilizers and found that 60% of a concentrated(2.5 mg/ml) unstable protein can be refolded using 40% glycerol as the best stabilizer. This procedure can be widely applicable for the refolding of unstable proteins.
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Report
(4 results)
Research Products
(4 results)
