structure and function of D-aspartic acid-specific endopeptidase

• Project/Area Number: 21580120
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Japan International Research Center for Agricultural Sciences
• Principal Investigator: NIRASAWA Satoru 独立行政法人国際農林水産業研究センター, 生物資源・利用領域, 主任研究員 (10343823)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Saori 秋田県総合食品研究センター, 食品加工研究所, 所長 (10142184)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: D型アミノ酸 / 酵素 / 細菌 / ペニシリン結合タンパク質 / ゲノム / 老化 / 抗生物質

Research Abstract

Paenidase is the first microorganism-derived D-aspartyl endopeptidase(Takahashi et al., J. Biochem. 2006). In this study, we revealed that the paenidase precursor was consisted of 322 amino acid residues of the mature region and 197 amino acid residues of the N-terminal extension peptide. Moreover, several mutants for the putative active site redidues of the paenidase were constructed, whereas they showed no peptidase activity. These results indicate that these residues of the paenidase are essential for the enzyme activity. We also discovered novel paenidase-inhibitors and crystallized paenidase.

Research Products

• [Journal Article] Purification and characterization of aspartic protease deriverd from Sf9 insect cells (2010)
  • Author(s): Takeshi Gotoh, Hiroki Ono, Ken-Ichi Kikuchi, Satoru Nirasawa, Saori Takahashi
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 74 Pages: 2154-2157
  • Peer Reviewed
• [Journal Article] Prorenin processing enzyme(PPE)produced by baculovirus-infected Sf-9 insect cells : PPE is the cysteine protease encoded in AcMNPV gene (2010)
  • Author(s): Takeshi Gotoh, Hirono Awa, Ken-Ichi Kikuchi, Satoru Nirasawa, Saori Takahashi
  • Journal Title: Bioscience, Biotechnology, and Biochemistry 74 Pages: 370-374
  • NAID: 10027551660
  • Peer Reviewed
• [Presentation] 原核微生物由来D-アスパラギン酸特的エンドペプチダーゼ (2012)
  • Author(s): 高橋砂織
  • Organizer: 平成23年度第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: 産総研つくばセンター共用講堂(茨城県つくば市)
  • Year and Date: 2012-01-31
• [Presentation] Protein isoasparatyl methyltransferase gene knock down induced human alveolar epithelial to mesenchymal cell transition through endoplasmic reticulum stress (2010)
  • Author(s): 小笠原正人
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-10
• [Presentation] 原核微生物由来D-アスパラギン酸エンドペプチダーゼ(Paenidase)遺伝子のゲノムライブラリーからの単離 (2010)
  • Author(s): 韮澤悟
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-07
• [Presentation] 原核微生物由来D-アスパラギン酸エンドペプチダーゼ(Paenidase)遺伝子のゲノムライブラリーからの単離、及び生産菌の全ゲノムシークエンス (2010)
  • Author(s): 韮澤悟
  • Organizer: 第6回D-アミノ酸研究会学術講演会
  • Place of Presentation: 富山国際会議場(富山市)
  • Year and Date: 2010-09-17
• [Presentation] 原核微生物由来D-アスパラギン酸エンドペプチダーゼ(Paenidase)の部位特異的変異法による特性解明 (2010)
  • Author(s): 韮澤悟
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス(東京都)
  • Year and Date: 2010-03-28
• [Presentation] Site-directed mutagenesis of bacterial D-aspartyl endopeptidase(paenidase) (2009)
  • Author(s): 韮澤悟
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-10
• [Presentation] Primary Structure and Functional Expression in Escherichia coli of Novel D-Aspartyl Endopeptidase, Paenidase, from Prokaryote (2009)
  • Author(s): 韮澤悟
  • Organizer: International Conference of D-Amino Acid Research(IDAR2009)
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2009-07-01