A novel role for the myrosinase-glucosinolate system in phototropic response

• Project/Area Number: 21580124
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: YAMADA Kosumi 筑波大学, 生命環境系, 准教授 (70292521)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 光屈性 / 植物成長調節物質 / ファイトアレキシン / 成長抑制物質 / 初期応答 / 成長調節物質

Research Abstract

Blue light induces many adaptive responses in plants, including the phototropism. To clarify the mechanism of growth inhibition in response to phototropic response, the isolation and identification of various growth inhibitors (PRS : phototropism regulating substances) has been attempted. To understand the mechanism of growth inhibition induced by PRS, physiological and genetic analyses have been performed. We found that phototropic stimulation induces H_2O_2 accumulation via the up-regulation of PRS (e.g. 4-MTBI and raphanusanins in radish, DIMBOA and MBOA in maize). We also found that PRSs contribute to their growth inhibitory activity by promoting H2_O_2 synthesis and subsequent lignification, which could increase the stiffness at the primary cell-wall level. These results suggest a close association between an oxidative burst and phototropic curvature.

Research Products

• [Journal Article] Identification and characterization of 2'-deoxyuridine from the supernatant of conidial suspensions of rice blast fungus as an infection-promoting factor in rice plants (2011)
  • Author(s): Ando, S., Sato, Y., Shigemori, H., Shimizu, T., Okada, K., Yamane, H., Jikumaru, Y., Kamiya, Y., Yamada, K., Akimoto-Tomiyama, C., Tanabe, S., Nishizawa, Y. and Minami, E
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 24 Pages: 519-532
  • Peer Reviewed
• [Journal Article] Structure-activity relationships on senescence-promoting effect of arabidopsides from Arabidopsis thaliana (2011)
  • Author(s): Haruyuki Nakajyo
  • Journal Title: HETEROCYCLES Volume: 83 Pages: 57-62
  • Peer Reviewed
• [Journal Article] Structure-activity relationships on senescence-promoting effect of arabidopsides from (2010)
  • Author(s): Nakajyo, H., Hisamatsu, Y., Goto, N., Yamada, K., Hasegawa, K. and Shigemori, H
  • Journal Title: Arabidopsis thaliana. Heterocycles Volume: 83 Pages: 57-62
  • Peer Reviewed
• [Journal Article] Comparative transcriptional profiling-based identification of raphanusanin-inducible genes (2010)
  • Author(s): Moehninsi, Miura, K., Nakajyo, H., Yamada, K., Hasegawa, K. and Shigemori, H
  • Journal Title: BMC Plant Biology Volume: 10 Pages: 111-127
  • Peer Reviewed
• [Journal Article] Magnaporthe oryzae : a tool for the molecular analysis of compatibility (2009)
  • Author(s): Ando, S., Tanabe, S., Shigemori, H., Yamada, K., Shimizu, T., Okada, K., Yamane, H., Akimoto-Tomiyama, C., Nishizawa, Y. and Minami, E
  • Journal Title: Journal of Pesticide Science Volume: 34 Pages: 335-338
  • NAID: 110007482310
  • Peer Reviewed
• [Journal Article] Magnaporthe oryzae : a tool for the molecular analysis of compatibility (2009)
  • Author(s): Ando, S., ら(他9名)
  • Journal Title: Journal of Pesticide Science 34 Pages: 335-338
  • Peer Reviewed
• [Presentation] 青色光照射によって誘導されるcell-wall stiffnessと光屈性との関連性 (2011)
  • Author(s): 山田小須弥, Riffat JABEEN, 長谷川剛, 長谷川宏司, 繁森英幸
  • Organizer: 植物化学調節学会第46回大会
  • Place of Presentation: 宇都宮大学
• [Presentation] 青色光照射によって誘導されるcell-wall stiffnessと光屈性との関連性 (2011)
  • Author(s): 山田小須弥
  • Organizer: 植物化学調節学会第46回大会
  • Place of Presentation: 宇都宮(宇都宮大学峰キャンパス)
• [Presentation] Identification and characterization of raphanusanin-induced genes in etiolated radish hypocotyls (2010)
  • Author(s): モーニンスィー, (他3名)
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(黒髪北キャンパス)
  • Year and Date: 2010-03-19
• [Presentation] The role of isothiocyanate and its derivatives in the blue light-induced growth inhibition of radish hypocotyls (2010)
  • Author(s): Kosumi Yamada, Taeko Narisawa, Moehninsi, Haruyuki Nakajyo, Tsuyoshi Hasegawa, Koji Hasegawa, Hideyuki Shigemori
  • Organizer: 8^<th> International Workshop Sulfur Metabolism in Higher Plants
  • Place of Presentation: Australia
• [Presentation] ダイコン下胚軸の光屈性におけるイソチオシアネートの役割 (2010)
  • Author(s): 成澤多恵子, 山田小須弥, モーニンスィー, 中城治之, 長谷川剛, 長谷川宏司, 繁森英幸
  • Organizer: 植物化学調節学会第45回大会
  • Place of Presentation: 神戸大学
• [Presentation] Identification and characterization of raphanusanin-induced genes in etiolated radish hypocotyls (2010)
  • Author(s): Moehninsi, Kosumi Yamada, Kenji Miura, Hideyuki Shigemori
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] ダイコン下胚軸の光屈性におけるイソチオシアネートの役割 (2010)
  • Author(s): 成澤多恵子, 他6名
  • Organizer: 植物化学調節学会第45回大会
  • Place of Presentation: 神戸(神戸大百年記念館)
• [Presentation] The role of isothiocyanate and its derivatives in the blue light-induced growth inhibition of radish hypocotyls (2010)
  • Author(s): Kosumi Yamada, 他6名
  • Organizer: 8^<th> International Workshop Sulfur Metabolism in Higher Plants
  • Place of Presentation: メルボルン(オーストラリア)
• [Presentation] Bioactive substances involved in apical dominance of peaseedlings (2010)
  • Author(s): Hideyuki Shigemori, 他6名
  • Organizer: Pacifichem 2010
  • Place of Presentation: ハワイ(アメリカ合衆国)
• [Book] 植物の感覚と運動、芽と葉の不思議、人間を助ける植物、「博士教えてください-植物の不思議-」 (2009)
  • Author(s): 山田小須弥, 長谷川宏司, 広瀬克利
  • Publisher: 大学教育出版