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Prediction of substrate specificity of enzymes based on sensitivity to a uniquely selective inhibitor.

Research Project

Project/Area Number 21580128
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionOkayama University

Principal Investigator

NITODA Teruhiko  岡山大学, 大学院・自然科学研究科, 准教授 (80284090)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsキチン / β-N-アセチルグルコサミニダーゼ / 酵素阻害剤 / TMG-chitotriomycin / 阻害選択性 / 糖質加水分解酵素ファミリー / 基質特異性 / 基質認識 / 生理的機能 / 生体内機能 / 触媒反応機構
Research Abstract

β-N-Acetylhexosaminidase(β-GlcNAcase), an enzyme widely distributed in organisms, is thought to be responsible for numerous physiological processes. However, most of the physiological functions remain to be clarified. The present study revealed that the newβ-GlcNAcase inhibitor TMG-chitotriomycin had the unique selectivity based on the substrate specificity ofβ-GlcNAcase, indicating that TMG-chitotriomycin can be used as a tool for classification ofβ-GlcNAcases differ in substrate specificity and even also in physiological functions.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (17 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Pochonicine, a polyhydroxylated pyrrolizidine alkaloid from fungus Pochonia suchlasporia var. suchlasporia TAMA 87 as a potentβ-N-acetylglucosaminidase inhibitor2009

    • Author(s)
      Hirokazu Usuki, Miho Toyo-oka, Hiroshi Kanzaki, Toru Okuda, Teruhiko Nitoda
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: Vol, 17 Pages: 7248-7253

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pochonicine, a polyhydroxylated pyrrolizidine alkaloid from fungus Pochonia suchlasporiavar. suchlasporia TAMA 87 as a potent β-N-acetylglucosaminidase inhibitor.2009

    • Author(s)
      Hirokazu Usuki
    • Journal Title

      Bioorganic & Medicinal Chemistry VOL. 17

      Pages: 7248-7253

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] β-N-Acetylglucosaminidase阻害物質TMG-chitotriomycinの阻害選択性に関する詳細検討2012

    • Author(s)
      塩田博人、臼木博一、神崎浩、仁戸田照彦
    • Organizer
      第37回日本農薬学会大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] β-N-Acetylglucosaminidase阻害物質TMG-chitotriomycinの阻害選択性2011

    • Author(s)
      塩田博人、臼木博一、神崎浩、仁戸田照彦
    • Organizer
      平成23年度日本農芸化学会西日本支部・中四国支部合同大会
    • Place of Presentation
      宮崎大学(宮崎市)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] β-N-Acetylglucosaminidase阻害物質TMG-chitotriomycinの阻害選択性2011

    • Author(s)
      塩田博人
    • Organizer
      平成23年度日本農芸化学会西日本支部中四国支部合同大会
    • Place of Presentation
      宮崎大学教育文化学部(宮崎市)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitory selectivity ofβ-N-acetylglucosaminidase inhibitor TMG-chitotriomycin produced by Streptomyces anulatus2011

    • Author(s)
      塩田博人、臼木博一、神崎浩、仁戸田照彦
    • Organizer
      国際微生物学連合2011会議
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Inhibitory selectivity of β-N-acetylglucosaminidase inhibitor TMG-chitotriomycin produced by Streptomyces anulatus2011

    • Author(s)
      塩田博人
    • Organizer
      国際微生物学連合2011会議(International Union of Microbiological Societies 2011 Congress)
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] キチン分解酵素を特異的に阻害する有機化合物2011

    • Author(s)
      仁戸田照彦
    • Organizer
      第10回バイオアカデミックフォーラム2011
    • Place of Presentation
      東京ビッグサイト(東京)
    • Year and Date
      2011-06-30
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] β-N-Acetylglucosaminidase阻害物質pochonicineとその類縁体の培養生産2011

    • Author(s)
      山田あさ美
    • Organizer
      日本農芸化学会中四国支部 第29回講演会
    • Place of Presentation
      徳島大学薬学部 (徳島市)
    • Year and Date
      2011-01-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Inhibitory properties ofβ-N-acetylglucosaminidase inhibitor TMG-chitotriomycin and structurally related compounds produced by Streptomyces anulatus2010

    • Author(s)
      塩田博人、行本千恵、臼木博一、神崎浩、仁戸田照彦
    • Organizer
      2010環太平洋国際会議
    • Place of Presentation
      ハワイコンベンションセンター(ホノルル市)
    • Year and Date
      2010-12-17
    • Related Report
      2011 Final Research Report
  • [Presentation] β-N-Acetylglucosaminidase inhibitor pochonicine and its analogs produced by the fungal strain Pochonia suchlasporia var.suchlasporia TAMA 872010

    • Author(s)
      山田あさ美
    • Organizer
      2010環太平洋国際会議
    • Place of Presentation
      ハワイコンベンションセンター(ホノルル市)
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Inhibitory properties of β-N-acetylglucosaminidase inhibitor TMG-chitotriomycin and structurally related compounds produced by Streptomyces anulatus2010

    • Author(s)
      塩田博人
    • Organizer
      2010環太平洋国際会議
    • Place of Presentation
      ハワイコンベンションセンター(ホノルル市)
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] β-N-アセチルグルコサミニダーゼ阻害物質生産菌Streptomyces anulatusによるキチナーゼ阻害物質の生産2010

    • Author(s)
      塩田博人
    • Organizer
      第14回生体触媒化学シンポジウムin静岡
    • Place of Presentation
      グランシップ静岡(静岡市)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 糸状菌が植物成分を材料に生産する新たな生理活性物質:グリコシダーゼ阻害剤pochonicineの例2010

    • Author(s)
      仁戸田照彦
    • Organizer
      おかやまバイオアクティブ研究会 第37回シンポジウム
    • Place of Presentation
      岡山理科大学(岡山市)
    • Year and Date
      2010-06-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] β-N-アセチルグルコサミニダーゼ阻害物質生産菌Streptomyces anulatusにより生産されるキチナーゼ阻害物質の予備的性状解析2010

    • Author(s)
      塩田博人
    • Organizer
      日本農芸化学会中四国支部 第27回講演会
    • Place of Presentation
      広島大学(東広島市)
    • Year and Date
      2010-06-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Search for Chitinolytic Enzyme Inhibitors of Microbial Origin2009

    • Author(s)
      仁戸田照彦
    • Organizer
      陝西師範大学化学与材料科学学院と岡山大学大学院自然科学研究科の国際交流協定締結記念式典およびシンポジウム
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2009-12-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 糸状菌由来β-N-acetylglucosaminidase阻害物質pochonicineの新規類縁体2009

    • Author(s)
      山田あさ美
    • Organizer
      第13回生体触媒化学シンポジウム
    • Place of Presentation
      香川大学幸町キャンパス(高松市)
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 糸状菌4菌株におけるβ-N-acetylglucosaminidase阻害物質生産培養条件の検討2009

    • Author(s)
      山田あさ美
    • Organizer
      日本農芸化学会中四国支部 第24回講演会
    • Place of Presentation
      くにびきメッセ(松江市)
    • Year and Date
      2009-05-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Novel polyhydroxylated pyrrolizidine alkaloid from a fungal strain grown on a plant-based solid medium and its potent β-N-acetylglucosaminidase inhibitory activity2009

    • Author(s)
      山田あさ美
    • Organizer
      日本学術振興会 アジア研究教育拠点事業 東アジア植物遺伝資源シンポジウム
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2009 Annual Research Report
  • [Book] Isolation, Identification and Characterization of Allelochemicals/Natural Products2009

    • Author(s)
      Teruhiko Nitoda, 他
    • Publisher
      Science Pub Inc
    • Related Report
      2011 Final Research Report
  • [Book] Isolation, Identification and Characterization of Allelochemicals/Natural Products(Chapter 17 : Bioassays : inhibitors of insect chitin-degrading enzymes)2009

    • Author(s)
      Teruhiko Nitoda
    • Total Pages
      13
    • Publisher
      Science Publishers
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_2.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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