Prediction of substrate specificity of enzymes based on sensitivity to a uniquely selective inhibitor.

• Project/Area Number: 21580128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Okayama University
• Principal Investigator: NITODA Teruhiko 岡山大学, 大学院・自然科学研究科, 准教授 (80284090)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: キチン / β-N-アセチルグルコサミニダーゼ / 酵素阻害剤 / TMG-chitotriomycin / 阻害選択性 / 糖質加水分解酵素ファミリー / 基質特異性 / 基質認識 / 生理的機能 / 生体内機能 / 触媒反応機構

Research Abstract

β-N-Acetylhexosaminidase(β-GlcNAcase), an enzyme widely distributed in organisms, is thought to be responsible for numerous physiological processes. However, most of the physiological functions remain to be clarified. The present study revealed that the newβ-GlcNAcase inhibitor TMG-chitotriomycin had the unique selectivity based on the substrate specificity ofβ-GlcNAcase, indicating that TMG-chitotriomycin can be used as a tool for classification ofβ-GlcNAcases differ in substrate specificity and even also in physiological functions.

Research Products

• [Journal Article] Pochonicine, a polyhydroxylated pyrrolizidine alkaloid from fungus Pochonia suchlasporia var. suchlasporia TAMA 87 as a potentβ-N-acetylglucosaminidase inhibitor (2009)
  • Author(s): Hirokazu Usuki, Miho Toyo-oka, Hiroshi Kanzaki, Toru Okuda, Teruhiko Nitoda
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: Vol, 17 Pages: 7248-7253
  • Peer Reviewed
• [Journal Article] Pochonicine, a polyhydroxylated pyrrolizidine alkaloid from fungus Pochonia suchlasporiavar. suchlasporia TAMA 87 as a potent β-N-acetylglucosaminidase inhibitor. (2009)
  • Author(s): Hirokazu Usuki
  • Journal Title: Bioorganic & Medicinal Chemistry VOL. 17 Pages: 7248-7253
  • Peer Reviewed
• [Presentation] β-N-Acetylglucosaminidase阻害物質TMG-chitotriomycinの阻害選択性に関する詳細検討 (2012)
  • Author(s): 塩田博人、臼木博一、神崎浩、仁戸田照彦
  • Organizer: 第37回日本農薬学会大会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2012-03-16
• [Presentation] β-N-Acetylglucosaminidase阻害物質TMG-chitotriomycinの阻害選択性 (2011)
  • Author(s): 塩田博人、臼木博一、神崎浩、仁戸田照彦
  • Organizer: 平成23年度日本農芸化学会西日本支部・中四国支部合同大会
  • Place of Presentation: 宮崎大学(宮崎市)
  • Year and Date: 2011-09-17
• [Presentation] β-N-Acetylglucosaminidase阻害物質TMG-chitotriomycinの阻害選択性 (2011)
  • Author(s): 塩田博人
  • Organizer: 平成23年度日本農芸化学会西日本支部中四国支部合同大会
  • Place of Presentation: 宮崎大学教育文化学部(宮崎市)
  • Year and Date: 2011-09-17
• [Presentation] Inhibitory selectivity ofβ-N-acetylglucosaminidase inhibitor TMG-chitotriomycin produced by Streptomyces anulatus (2011)
  • Author(s): 塩田博人、臼木博一、神崎浩、仁戸田照彦
  • Organizer: 国際微生物学連合2011会議
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-08
• [Presentation] Inhibitory selectivity of β-N-acetylglucosaminidase inhibitor TMG-chitotriomycin produced by Streptomyces anulatus (2011)
  • Author(s): 塩田博人
  • Organizer: 国際微生物学連合2011会議(International Union of Microbiological Societies 2011 Congress)
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-08
• [Presentation] キチン分解酵素を特異的に阻害する有機化合物 (2011)
  • Author(s): 仁戸田照彦
  • Organizer: 第10回バイオアカデミックフォーラム2011
  • Place of Presentation: 東京ビッグサイト(東京)
  • Year and Date: 2011-06-30
• [Presentation] β-N-Acetylglucosaminidase阻害物質pochonicineとその類縁体の培養生産 (2011)
  • Author(s): 山田あさ美
  • Organizer: 日本農芸化学会中四国支部 第29回講演会
  • Place of Presentation: 徳島大学薬学部 (徳島市)
  • Year and Date: 2011-01-22
• [Presentation] Inhibitory properties ofβ-N-acetylglucosaminidase inhibitor TMG-chitotriomycin and structurally related compounds produced by Streptomyces anulatus (2010)
  • Author(s): 塩田博人、行本千恵、臼木博一、神崎浩、仁戸田照彦
  • Organizer: 2010環太平洋国際会議
  • Place of Presentation: ハワイコンベンションセンター(ホノルル市)
  • Year and Date: 2010-12-17
• [Presentation] β-N-Acetylglucosaminidase inhibitor pochonicine and its analogs produced by the fungal strain Pochonia suchlasporia var.suchlasporia TAMA 87 (2010)
  • Author(s): 山田あさ美
  • Organizer: 2010環太平洋国際会議
  • Place of Presentation: ハワイコンベンションセンター(ホノルル市)
  • Year and Date: 2010-12-17
• [Presentation] Inhibitory properties of β-N-acetylglucosaminidase inhibitor TMG-chitotriomycin and structurally related compounds produced by Streptomyces anulatus (2010)
  • Author(s): 塩田博人
  • Organizer: 2010環太平洋国際会議
  • Place of Presentation: ハワイコンベンションセンター(ホノルル市)
  • Year and Date: 2010-12-17
• [Presentation] β-N-アセチルグルコサミニダーゼ阻害物質生産菌Streptomyces anulatusによるキチナーゼ阻害物質の生産 (2010)
  • Author(s): 塩田博人
  • Organizer: 第14回生体触媒化学シンポジウムin静岡
  • Place of Presentation: グランシップ静岡(静岡市)
  • Year and Date: 2010-09-23
• [Presentation] 糸状菌が植物成分を材料に生産する新たな生理活性物質:グリコシダーゼ阻害剤pochonicineの例 (2010)
  • Author(s): 仁戸田照彦
  • Organizer: おかやまバイオアクティブ研究会 第37回シンポジウム
  • Place of Presentation: 岡山理科大学(岡山市)
  • Year and Date: 2010-06-11
• [Presentation] β-N-アセチルグルコサミニダーゼ阻害物質生産菌Streptomyces anulatusにより生産されるキチナーゼ阻害物質の予備的性状解析 (2010)
  • Author(s): 塩田博人
  • Organizer: 日本農芸化学会中四国支部 第27回講演会
  • Place of Presentation: 広島大学(東広島市)
  • Year and Date: 2010-06-05
• [Presentation] Search for Chitinolytic Enzyme Inhibitors of Microbial Origin (2009)
  • Author(s): 仁戸田照彦
  • Organizer: 陝西師範大学化学与材料科学学院と岡山大学大学院自然科学研究科の国際交流協定締結記念式典およびシンポジウム
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2009-12-17
• [Presentation] 糸状菌由来β-N-acetylglucosaminidase阻害物質pochonicineの新規類縁体 (2009)
  • Author(s): 山田あさ美
  • Organizer: 第13回生体触媒化学シンポジウム
  • Place of Presentation: 香川大学幸町キャンパス(高松市)
  • Year and Date: 2009-12-03
• [Presentation] 糸状菌4菌株におけるβ-N-acetylglucosaminidase阻害物質生産培養条件の検討 (2009)
  • Author(s): 山田あさ美
  • Organizer: 日本農芸化学会中四国支部 第24回講演会
  • Place of Presentation: くにびきメッセ(松江市)
  • Year and Date: 2009-05-23
• [Presentation] Novel polyhydroxylated pyrrolizidine alkaloid from a fungal strain grown on a plant-based solid medium and its potent β-N-acetylglucosaminidase inhibitory activity (2009)
  • Author(s): 山田あさ美
  • Organizer: 日本学術振興会 アジア研究教育拠点事業 東アジア植物遺伝資源シンポジウム
  • Place of Presentation: 岡山大学(岡山市)
• [Book] Isolation, Identification and Characterization of Allelochemicals/Natural Products (2009)
  • Author(s): Teruhiko Nitoda, 他
  • Publisher: Science Pub Inc
• [Book] Isolation, Identification and Characterization of Allelochemicals/Natural Products(Chapter 17 : Bioassays : inhibitors of insect chitin-degrading enzymes) (2009)
  • Author(s): Teruhiko Nitoda
  • Total Pages: 13
  • Publisher: Science Publishers
• [Remarks] ホームページ
  • URL: http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_2.html