Elucidation of the role of MT-SP1 in the intestinal epithelial turnover and exploration of food components that control the activation of the molecule.

• Project/Area Number: 21580138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kyoto University
• Principal Investigator: TSUZUKI Satoshi 京都大学, 大学院・農学研究科, 助教 (50283651)
• Co-Investigator(Kenkyū-buntansha): INOUE Kazuo 京都大学, 大学院・農学研究科, 准教授 (80213148)
• Co-Investigator(Renkei-kenkyūsha): INOUYE Kuniyo 京都大学, 大学院・農学研究科, 教授 (10223249)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 腸上皮 / 代謝回転 / 基底膜 / MT-SP1 / 活性化制御

Research Abstract

We found that a membrane-bound serine protease MT-SP1 induces detachment and apoptosis of intestinal epithelial IEC-6 cells. Also, we found that a recombinant form of MT-SP1 pseudozymogen exhibits a protease activity and that the activity is not seen in the presence of NaCl.

Research Products

• [Journal Article] A recombinant catalytic domain ofmatriptase induces detachment and apoptosis of small-intestinal epithelial IEC-6 cells cultured on laminin coated surface (2010)
  • Author(s): Mochida S, Tsuzuki, S, Inouye K, Fushiki T.
  • Journal Title: J. Biochem. Volume: 148 Issue: 6 Pages: 721-732
  • DOI: 10.1093/jb/mvq108
  • Peer Reviewed
• [Journal Article] The Structural Requirements of Matriptase in Its Ectodomain Release in Polarized Epithelial Cells (2010)
  • Author(s): Tsuzuki S, Murai N, Miyake Y, Inouye K, Fushiki T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 74 Issue: 6 Pages: 1295-1297
  • DOI: 10.1271/bbb.100074
  • NAID: 10027556665
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Matriptase Does Not Require Hepatocyte Growth Factor Activator Inhibitor Type-1 for Activation in an Epithelial Cell Expression Model (2010)
  • Author(s): Miyake Y, Tsuzuki S, Fushiki T, Inouye K.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 74 Issue: 4 Pages: 848-850
  • DOI: 10.1271/bbb.90696
  • NAID: 10027554197
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] The optimal activity of a pseudozymogen form of recombinantmatriptase under the mildly acidic pHand low ionic strength conditions (2010)
  • Author(s): Inouye K, Yasumoto M, Tsuzuki S, Mochida S, Fushiki T.
  • Journal Title: J. Biochem. Volume: 147 Issue: 4 Pages: 485-492
  • DOI: 10.1093/jb/mvp190
  • Peer Reviewed
• [Journal Article] The role of asparagine-linked glycosylation site on thecatalytic domain of matriptase in itszymogen activation (2010)
  • Author(s): Miyake Y, Tsuzuki S, Mochida S, Fushiki T, Inouye K.
  • Journal Title: Biochim. Biophys. Acta Volume: 1804 Issue: 1 Pages: 156-165
  • DOI: 10.1016/j.bbapap.2009.09.025
  • Peer Reviewed
• [Journal Article] Granzyme A and thrombin differentially promote the release of interleukin-8 from alveolar epithelial A549 cells. (2010)
  • Author(s): Yoshikawa Y, Hirayasu H, Tsuzuki S, Fushiki T
  • Journal Title: Cytotechnology Volume: 62 Pages: 325-332
  • Peer Reviewed
• [Journal Article] The structural requirements of matriptase in its ectodomain release in polarized epithelial cells. (2010)
  • Author(s): Tsuzuki S, Murai N, Miyake Y, Inouye K, Fushiki T
  • Journal Title: Biosci Biotechnol Biochem Volume: 74 Pages: 1295-1297
  • NAID: 10027556665
  • Peer Reviewed
• [Journal Article] A recombinant catalytic domain of matriptase induces detachment and apoptosis of small-intestinal epithelial IEC-6 cells cultured on laminin-coated surface. (2010)
  • Author(s): Mochida S, Tsuzuki S, Inouye K, Fushiki T
  • Journal Title: J Biochem Volume: 148 Pages: 721-732
  • NAID: 10027879947
  • Peer Reviewed
• [Journal Article] The role of asparagine-linked glycosylation site on the catalytic domain of matriptase in its zymogen activation (2010)
  • Author(s): Miyake Y, Tsuzuki S, Mochida S, Fushiki T, Inouye K
  • Journal Title: Biochim. Biophys. Acta 1804 Pages: 156-165
  • Peer Reviewed
• [Journal Article] The optimal activity of a pseudozymogen form of recombinant matriptase under the mildly acidic pH and low ionic strength conditions (2010)
  • Author(s): Inouye K, Yasumoto M, Tsuzuki S, Mochida S, Fushiki T
  • Journal Title: J. Biochem. 147 Pages: 485-492
  • NAID: 10027874930
  • Peer Reviewed
• [Journal Article] Matriptase does not require hepatocyte growth factor activator inhibitor type-1 for activation in an epithelial cell expression model (2010)
  • Author(s): Miyake Y, Tsuzuki S, Fushiki T, Inouye K
  • Journal Title: Biosci. Biotechnol. Biochem. (in press)
  • NAID: 10027554197
  • Peer Reviewed
• [Journal Article] The structural requirements of matriptase in its ectodomain release in polarized epithelial cells (2010)
  • Author(s): Tsuzuki S, Murai N, Miyake Y, Inouye K, Fushiki T
  • Journal Title: Biosci. Biotechnol. Biochem. (in press)
  • NAID: 10027556665
  • Peer Reviewed
• [Journal Article] The Occurrence of Matriptase C-Terminal Fragments on the Apical and Basolateral Sides of Madin-Darby Canine Kidney Epithelial Cells (2009)
  • Author(s): Tsuzuki S, Miyake Y, Inouye K, Fushiki T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 73 Issue: 11 Pages: 2538-2540
  • DOI: 10.1271/bbb.90431
  • NAID: 10027548270
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Requirement of the activity of hepatocyte growth factor activator inhibitor type 1 for the extracellular appearance of a trans-membrane serine protease matriptase in monkey kidney COS-1 cells. (2009)
  • Author(s): Miyake Y, Tsuzuki S, Yasumoto M, Fushiki T Inouye K.
  • Journal Title: Cytotechnology Volume: 60 Pages: 95-103
  • Peer Reviewed
• [Journal Article] Involvement of the cytoplasmic juxtamembrane region of matriptase in its exclusive localization to the basolateral membrane domain of Madin-Darby canine kidney epithelial cells. (2009)
  • Author(s): Murai N, Miyake Y, Tsuzuki S, Inouye K Fushiki T
  • Journal Title: Cytotechnology Volume: 53 Issue: 3 Pages: 169-176
  • DOI: 10.1007/s10616-009-9205-0
  • Peer Reviewed
• [Journal Article] Secreted expression of pseudozymogen forms of recombinant matriptase in Pichia Pastoris (2009)
  • Author(s): Mochida S, Tsuzuki S, Yasumoto M, Inouye K, Fushiki T
  • Journal Title: Enzyme Microb. Technol Volume: 45 Issue: 4 Pages: 288-294
  • DOI: 10.1016/j.enzmictec.2009.06.008
  • Peer Reviewed
• [Journal Article] Activation of amembrane-bound serine protease matriptaseon the cell surface (2009)
  • Author(s): Miyake Y, Yasumoto M, Tsuzuki S, Fushiki T, Inouye K
  • Journal Title: J. Biochem. Volume: 146
  • Peer Reviewed
• [Journal Article] Activation of a Membrane-bound serine protease matriptase on the cell surface (2009)
  • Author(s): Miyake Y, Yasumoto M, Tsuzuki S, Fushiki T, Inouye K
  • Journal Title: J. Biochem. 146 Pages: 783-790
  • NAID: 10026302976
  • Peer Reviewed
• [Journal Article] Secreted expression of pseudozymogen forms of recombinant matriptase in Pichia Pastoris (2009)
  • Author(s): Mochida S, Tsuzuki S, Yasumoto M, Inouye K, Fushiki T
  • Journal Title: Enzyme Microb. Technol. 45 Pages: 288-294
  • Peer Reviewed
• [Journal Article] Involvement of the cytoplasmic juxtamembrane region of matriptase in its exclusive localization to the basolateral membrane domain of Madin-Darby canine Kidney epithelial cells (2009)
  • Author(s): Murai N, Miyake Y, Tsuzuki S, Inouye K, Fushiki T
  • Journal Title: Cytotechnology 53 Pages: 169-176
  • Peer Reviewed
• [Journal Article] Requirement of the activity of hepatocyte growth factor activator inhibitor type 1 for the extracelular appearance of a transmembrane serine protease matriptase in monkey kidney COS-1 cells (2009)
  • Author(s): Miyake Y, Tsuzuki S, Yasumoto M, Fushiki T, Inouye K
  • Journal Title: Cytotechnology 60 Pages: 95-103
  • Peer Reviewed
• [Journal Article] The occurrence of matriptase C-terminal fragments on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells (2009)
  • Author(s): Tsuzuki S, Miyake Y, Inouye K, Fushiki T
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 2538-2540
  • NAID: 10027548270
  • Peer Reviewed
• [Presentation] 小腸上皮細胞の剥離を促すセリンプロテアーゼと硫酸化多糖による阻害 (2011)
  • Author(s): 都築巧
  • Organizer: Hindgut Club JAPANシンポジウム(招待講演)
  • Place of Presentation: 東京(専修大学)
  • Year and Date: 2011-12-17
• [Presentation] 小腸上皮細胞の剥離を促すセリンプロテアーゼと硫酸化多糖による阻害 (2011)
  • Author(s): 都築巧
  • Organizer: Hindgut Club JAPANシンポジウム
  • Place of Presentation: 東京(専修大学)(招待講演)
  • Year and Date: 2011-12-17
• [Presentation] マトリプターゼ前駆体活性発現におけるLDL受容体ドメインの意義の解明 (2011)
  • Author(s): 友石満里絵
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都(京都女子大学)
  • Year and Date: 2011-03-27
• [Presentation] Matriptaseのstem領域においてHAI-1(hepatocyte growth factor activator inhibitor type 1) (2010)
  • Author(s): 安元誠
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京(東京大学)
  • Year and Date: 2010-03-28
• [Presentation] MatriptaseはMMPsを介してラット小腸由来IEC-6細胞のアポトーシスを誘導する (2010)
  • Author(s): 持田誠也
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京(東京大学)
  • Year and Date: 2010-03-28
• [Presentation] Matriptaseのstem領域においてHAI-1(hepatocyte growth factor activator inhibitor type 1)との相互作用を容易にする部位の同定 (2010)
  • Author(s): 安元誠, ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京(東京大学)
  • Year and Date: 2010-03-28
• [Presentation] 肝細胞増殖因子アクチベーター阻害物質I型(HAI-1)によるマトリプターゼ阻害機構 (2009)
  • Author(s): 都築巧
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2009-10-24
• [Presentation] 肝細胞増殖因子アクチベーター阻害物質I型 (HAI-1)によるマトリプターゼ阻害機構 (2009)
  • Author(s): 都築巧, ら
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2009-10-24
• [Presentation] Matriptaseのstem領域においてHAI-1(hepatocyte growth factor activator inhibitor type 1) (2009)
  • Author(s): 安元誠
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2009-10-23
• [Presentation] Matriptaseのstem領域においてHAI-1(hepatocyte growth factor activator inhibitor type 1)との相互作用を促進する部位の同定 (2009)
  • Author(s): 安元誠, ら
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2009-10-23