Cardioprotective effects of nicotinic acid via Sirt1 activation

• Project/Area Number: 21580139
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Shimane University
• Principal Investigator: HARA Nobumasa 島根大学, 医学部, 講師 (20284028)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ニコチン酸 / NAD / Sirt1 / 心筋細胞 / ストレス / 細胞死 / 高脂血症薬

Research Abstract

Nicotinic acid(NA) exhibits various pharmacological actions including cardioprotection against oxidative stress. However, mechanisms underlying the reported actions of NA remain unclear. We have found that NA increases cellular NAD contents in primary rat cardiomyocytes and that the vitamin protects the cardiomyocytes against oxidative stress-induced damage by increasing cellular NAD contents.

Research Products

• [Journal Article] Nicotinamide phospho-ribosyltransferase/Visfatin does not catalyze nicotinamide mononucleotide formation in blood plasma (2011)
  • Author(s): Hara N, Yamada K, Shibata T, Osago H, Tsuchiya M
  • Journal Title: PLoS ONE Volume: Vol 6, No.8
  • Peer Reviewed
• [Journal Article] Nicotinamide phosphoribosyltransferase/visfatin does not catalyze nicotinamide mononucleotide formation in blood plasma (2011)
  • Author(s): Nobumasa Hara
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Interferon-γelevates nicotinamide N-methyltransferase activity and nicotinamide level in human glioma cells (2010)
  • Author(s): Yamada K, Miyazaki T, Hara N, Tsuchiya M
  • Journal Title: J. Nutr. Sci. Vitaminol Volume: Vol 56, No.2 Pages: 83-86
  • NAID: 10026476527
  • URL: http://dx.doi.org/10.3177/jnsv.56.83
  • Peer Reviewed
• [Journal Article] Interferon-γ elevates nicotinamide N-methyltransferase activity and nicotinamide level in human glioma cells (2010)
  • Author(s): Kazuo Yamada
  • Journal Title: J.Nutr.Sci.Vitaminol. Volume: 56 Pages: 83-86
  • Peer Reviewed
• [Presentation] ニコチン酸の薬理作用は細胞内NADレベルの増加を介するか? (2011)
  • Author(s): 原伸正, 山田和夫, 柴田朋子, 長子晴美, 土屋美加子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都市)
• [Presentation] LC/MS/MSを用いた細胞の酸処理による酸化および還元型NADPの同時定量法の確立 (2011)
  • Author(s): 山田和夫, 原伸正, 長子晴美, 土屋美加子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都市)
• [Presentation] Visfatin/nicotinamide phosphoribosyltransferase does not function as an extracellular NAD synthetic enzyme (2010)
  • Author(s): Hara N, Yamada K, Osago H, Shibata T, Tsuchiya M
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(神戸市)
• [Presentation] 質量分析によるNADH酸分解産物を用いたNADH定量法とこの方法の細胞の酸化還元状態の解析への応用の検討 (2010)
  • Author(s): 山田和夫, 原伸正, 長子晴美, 柴田朋子, 土屋美加子
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(神戸市)
• [Presentation] Visfatin/nicotinamide phosphoribosyltransferase dose not function as an extracellular NAD synthetic enzyme (2010)
  • Author(s): 原伸正
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 質量分析によるNADH酸分解産物を用いたNADH定量法とこの方法の細胞の酸化還元状態の解析への応用の検討 (2010)
  • Author(s): 山田和夫
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Quantitative determination of NAD turnover using deuterium-labeled nicotinamide and nicotinic acid (2009)
  • Author(s): 原伸正, 山田和夫, 柴田朋子, 長子晴美, 土屋美加子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
• [Presentation] (招待講演) Elevation of Cellular NAD Levels by Nicotinic Acid and Involvement of Nicotinic Acid Phosphoribosyltransferase in Human cells (2009)
  • Author(s): Hara N, Yamada K, Shibata K, Osago H, Tsuchiya M
  • Organizer: NAD METABOLISM AND SIGNALING
  • Place of Presentation: CAREFREE, ARIZONA, USA
• [Presentation] Elevation of Cellular NAD Levels by Nicotinic Acid and Involvement of Nicotinic Acid Phosphoribosyltransferase in Human Cells (2009)
  • Author(s): Hara N.(招待講演)
  • Organizer: NAD METABOLISM AND SIGNALING
  • Place of Presentation: CAREFREE, ARIZONA
• [Presentation] Measurement of the Precursors of NAD Biosynthesis in Mammalian Cells (2009)
  • Author(s): Yamada K.
  • Organizer: NAD METABOLISM AND SIGNALING
  • Place of Presentation: CAREFREE, ARIZONA
• [Presentation] Quantitative determination of NAD turnover using deuterium-labeled nicotinamide and nicotinic aced (2009)
  • Author(s): 原伸正
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Tight regulation of the intracellular nicotinamide concentration in human cells (2009)
  • Author(s): 山田和夫
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸