| Project/Area Number |
21580148
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUMAZAWA Shigenori 静岡県立大学, 食品栄養科学部, 教授 (10295561)
ISHII Takeshi 静岡県立大学, 食品栄養科学部, 助教 (50448700)
NAITO Akira 横浜国立大学, 工学研究院, 教授 (80172245)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 食品化学 / カテキン / 化学生物学 / タンパク質 / リン脂質 / REDOR / NMR / ECg / DOSY / ケミカルバイオロジー |
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| Research Abstract |
1.The association constants obtained from the frequency changes of quartz-crystal microbalance(QCM) revealed interactions of ECg and EGCg with HSA that are 100 times stronger than those of EC and EGC.
2.The most important structural element contributing to the formation of protein carbonyl in HSA by tea catechins is the pyrogallol structural motif in the B-ring, followed by the galloyl group. By both mass spectrometry and electrophoresis/blotting with redox-cycling staining, we revealed that pyrogallol-type catechins had higher reactivity with protein thiols than catechol-type catechins.
3.We synthesized[^<13> C]-ECg, in which the carbonyl carbon of the galloyl moiety was labeled by^<13> C isotope, and analyzed it by solid-state NMR spectroscopy. Solid-state^<31> P NMR analysis indicated that ECg changes the gel-to-liquid-crystalline phase transition temperature of DMPC bilayers as well as the dynamics and mobility of the phospholipids. The accurate intermolecular. interatomic distance between the labeled carbonyl carbon of[^<13> C]-ECg and the phosphorus of the phospholipid was determined to be 5.3±0.1 A by^<13> C.^<31> P rotational echo double resonance(REDOR) measurements.
4.The DEAD-box RNA helicase p68, which is overexpressed in a variety of tumor cells and plays an important role in cancer development and progression, was identified as a novel EGCG-binding target.
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