Establishment of the diagnostic technique of nutritional status with the nutriproteomics of the skin

• Project/Area Number: 21580156
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: OISHI Yuichi 東京農業大学, 応用生物科学部, 教授 (00313073)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 二次元電気泳動 / ニュートリプロテオミクス / 表皮タンパク質 / MALDI-TOF-MS / NALDI-TOF-MS / 二次元電気栄堂

Research Abstract

I focused on the proteomic analysis of epidermal proteins because of developing a new clinical examination technique to evaluate a nutrition status. I investigated the change in epidermal proteins in rats fed diets with different protein quality and quantity(casein diet, gluten diet and protein-free diet) using 2-two-dimensional electrophoresis(2-DE). This 2-DE result showed that 21 types of proteins underwent a change by the diets with low protein quality containing Ras-related protein Rab-6A, NADH dehydrogenase, α-enolase.

Research Products

• [Journal Article] Adiponectin promotes hyaluronan synthesis along with increases in hyaluronan synthase 2 transcripts through an AMP-activated protein kinase/peroxisome proliferator-activated receptor-a-dependent pathway in human dermal fibroblasts (2011)
  • Author(s): 山根拓実、服部一夫、大石祐一
  • Journal Title: Biochemical and Biophysical Research Volume: 415 Pages: 235-238
  • Peer Reviewed
• [Journal Article] A high-fat diet reduces ceramide synthesis by decreasing adiponectin levels and decreases lipid content by HMG-CoA reductase and CPT-1 mRNA expression in the skin (2011)
  • Author(s): 山根拓実、服部一夫、大石祐一
  • Journal Title: Molecular Nutrition and Food Research Volume: 55巻 Issue: S2
  • DOI: 10.1002/mnfr.201100144
  • Peer Reviewed
• [Journal Article] High-fat diet reduces levels of type I tropocollagen and hyaluronan in rat skin (2010)
  • Author(s): 山根拓実, 服部一夫, 大石祐一, 滝田聖親
  • Journal Title: Molecular Nutrition and Food Research Volume: 54巻
  • Peer Reviewed
• [Presentation] 表皮角化細胞におけるTGF-β1のセラミド合成への影響 (2012)
  • Author(s): 村松愛美、山根拓実、服部一夫、大石祐一
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-23
• [Presentation] アディポネクチンが皮膚中ヒアルロナン合成に及ぼす影響 (2011)
  • Author(s): 山根拓実、服部一夫、大石祐一
  • Organizer: 日本食品科学工学会
  • Place of Presentation: 東北大学
• [Presentation] ラットの皮膚に及ぼす食餌中油脂原の影響 (2011)
  • Author(s): 山根拓実、鈴木真里菜、松川寛紀、服部一夫、大石祐一
  • Organizer: 日本栄養・食糧学会
  • Place of Presentation: 御茶ノ水女子大学
• [Presentation] タンパク質栄養条件の違いにより変動する表皮タンパク質のプロテオーム解析 (2011)
  • Author(s): 藤田尚子、岡田歩美、町田千代美、服部一夫、大石祐一
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学
• [Presentation] Adiponectinが皮膚中I型コラーゲン合成に及ぼす影響 (2011)
  • Author(s): 山根拓実、佐藤ますみ、坂本真美、服部一夫、大石祐一
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学
• [Presentation] 表皮角化細胞におけるTGF-β1のセラミド合成への影響 (2011)
  • Author(s): 村松愛美、山根拓実、服部一夫、大石祐一
  • Organizer: 日本農芸化学会
  • Place of Presentation: 日本農芸化学会
• [Presentation] 滝田聖親AdiponectinがI型コラーゲンおよびヒアルロナン合成酵素2遺伝子発現に及ぼす影響 (2010)
  • Author(s): 山根拓実、服部一夫、大石祐一
  • Organizer: 日本栄養・食糧学会
  • Place of Presentation: 長崎ブリックホール