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Studies on the mechanism of functional food components at molecular basis focusing on phosphorylation hub protein.

Research Project

Project/Area Number 21580157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionNihon University

Principal Investigator

SEKI Taiichiro  日本大学, 生物資源科学部, 教授 (20187834)

Co-Investigator(Kenkyū-buntansha) ARIGA Toyohiko  日本大学, 生物資源科学部, 教授 (50096757)
HOSONO Takashi  日本大学, 生物資源科学部, 助手 (80445741)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords食品機能 / garlic / apoptosis / heat shock protein / Sulfide / Food function / MAPK / food function
Research Abstract

Consumption of a certain amount of vegetables and fruits negatively correlates with the incidence of life-style related diseases including cancer. To clarify the anti-cancer effect of phytochemicals derived from vegetables and fruits at molecular basis, we focused on phosphorylation-hub protein which converts various intracellular stimuli into the phosphorylation signals. Apoptosis signal-regulating kinase 1(ASK1), a typical phosphorylation hub protein locating at the up-stream of stress-responsible kinase, SAPK/JNK, converts the intracellular stimuli to phosphorylation signals ; ASK1 is also a sort of MAPKK. In the quiescent cells, intracellular ASK1 binds with thioredoxin(Trx) to inactivate its biological activity ; oxidation of cysteine residues in Trx causes the activation of ASK1. In this study, we demonstrated for the first time that garlic derived anti-cancer compound, diallyl trisulfide, formed disulfide bond between cysteine residues in Trx molecule, followed by activation of ASK1 and formation of so-called signalsome in leukemic cells. This oxidative modification by diallyl trisulfide of the cysteine residues is a causative of apoptosi in the cells.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (41 results)

All 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (25 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Anticancer Property of Allyl Sulfides Derived from Garlic(Allium sativum L.)2012

    • Author(s)
      Seki T, Hosono T, Suda S, Kimura K, Ariga T.
    • Journal Title

      J Food Drug Anal

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Beneficial effects of Cinnamon on the metabolic syndrome, inflammation, and pain, and mechanisms underlying these effects-a review2012

    • Author(s)
      Shen Y
    • Journal Title

      Journal of Traditional and Complementary Medicine

      Volume: 2(1) Pages: 27-32

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anticancer property of ally1 sulfides derived from Garlic (Allium sativum L.)2012

    • Author(s)
      Seki T
    • Journal Title

      Journal of Food & Drug Analysis

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between lipophilicity and inhibitory activity against cancer cell growth of 9 kinds of alk(en) yl trisulfides with different side-chains2010

    • Author(s)
      Iitsuka Y, Tanaka Y, Hosono-Fukao T, Hosono T, Seki T, Ariga T.
    • Journal Title

      Oncology Research

      Volume: 19 Pages: 575-82

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between lipophilicity and inhibitory activity against cancer cell growth of 9 kinds of alk(en)yl trisulfides with different side-chains.2010

    • Author(s)
      Iitsuka Y
    • Journal Title

      Oncology Research

      Volume: 19 Pages: 575-82

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diallyl trisulfide protects rats from carbon tetrachloride-induced liver injury2009

    • Author(s)
      Hosono-Fukao T, Hosono T, Seki T, Ariga T.
    • Journal Title

      J Nutr

      Volume: 139(12) Pages: 2252-6

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibacterial potential of garlic-derived allicin and its cancellation by sulfhydryl compounds2009

    • Author(s)
      Fujisawa H, Watanabe K, Suma K, Origuchi K, Matsufuji H, Seki T, Ariga T.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 73(9) Pages: 1948-55

    • NAID

      10027545286

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diallyl sulfides in garlic activate both TRPA1 and TRPV12009

    • Author(s)
      Koizumi K, Iwasaki Y, Narukawa M, Iitsuka Y, Fukao T, Seki T, Ariga T, Watanabe T.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 382(3) Pages: 545-8

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diallyl sulfides in garlic activate both TRPA1 and TRPV12009

    • Author(s)
      Koizumi K
    • Journal Title

      Biochem Biophys Res Commun 382(3)

      Pages: 545-8

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antibacterial potential of garlic-derived allicin and its cancellation by sulfhydryl compounds2009

    • Author(s)
      Fujisawa H
    • Journal Title

      Biosci Biotechnol Biochem 73(9)

      Pages: 1948-55

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diallyl trisulfide protects rats from carbon tetrachloride-induced liver injury2009

    • Author(s)
      Hosono-Fukao T
    • Journal Title

      J Nutr. 139(12)

      Pages: 2252-6

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Anticancer effect of sulfur-containing compounds derived from garlic2011

    • Author(s)
      Seki T, Hosono T, Hosono-Fukao T, Ariga T.
    • Organizer
      International Conference on Food Factors for Health Promotion(ICoFF2011)
    • Place of Presentation
      Taipei International Convention Center, Taipei, Taiwan
    • Year and Date
      2011-11-22
    • Related Report
      2011 Final Research Report
  • [Presentation] Anticancer effect of sulfur-containing compounds derived from garlic2011

    • Author(s)
      Taiichiro Seki
    • Organizer
      International Conference on Food Factors for Health Promotion (ICoFF2011) Invited Speaker
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2011-11-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification of novel molecular target of diallyl trisulfide in U937 cells2011

    • Author(s)
      Suda S, Tanaka Y, Watanabe K, Hosono T, Ogihara J, Ariga T, Seki T.
    • Organizer
      International Conference on Food Factors for Health Promotion(ICoFF2011)
    • Place of Presentation
      Taipei International Convention Center, Taipei, Taiwan
    • Year and Date
      2011-11-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Taiichiro Seki Identification of novel molecular target of dially1 trisulfide in U937 cells2011

    • Author(s)
      Shun Suda
    • Organizer
      International Conference on Food Factors for Health Promotion (ICoFF2011)
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Diallyl trisulfideによるCYP2E1活性抑制機構の解析2011

    • Author(s)
      細野崇、内田真弓子、細野(深尾)友美、関泰一郎、有賀豊彦
    • Organizer
      第26回日本香辛料研究会学術講演会
    • Place of Presentation
      福井
    • Year and Date
      2011-11-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Dially1 trisulfideによるCYP2E1活性抑制機構の解析2011

    • Author(s)
      細野崇
    • Organizer
      第26回日本香辛料研究会学術講演会
    • Place of Presentation
      福井
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] Garlic Compounds and Their Biological Effects and Stability, Symposium for Functional Black Garlic, Food Biosciences : Promise and Challenging in the Future2011

    • Author(s)
      Seki T, Hosono T, Ariga T.
    • Organizer
      2011 Annual Meeting of Korean Society of Food Science and Technology
    • Place of Presentation
      EXCO, Daegu, Korea
    • Year and Date
      2011-06-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Garlic Compounds and Their Biological Effects and Stability2011

    • Author(s)
      Taiichiro Seki
    • Organizer
      2011 Annual Meeting of Korean Society of Food Science and Technology
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2011-06-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] ガーリック由来香気成分diallyl trisulfideはシステイン残基との反応を介してヒト大腸がん細胞の増殖を抑制する2011

    • Author(s)
      細野崇、田中友紀、深尾友美、新藤一敏、関泰一郎、有賀豊彦
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-14
    • Related Report
      2011 Final Research Report
  • [Presentation] ガーリック由来香気成分Diallyl trisulfideの白血病細胞における分子標的の解明2011

    • Author(s)
      須田峻、田中理江、田中友紀、細野崇、荻原淳、関泰一郎、有賀豊彦
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-14
    • Related Report
      2011 Final Research Report
  • [Presentation] ガーリック由来香気成分dially1 trisulfideはシステイン残基との反応を介してヒト大腸がん細胞の増殖を抑制する2011

    • Author(s)
      細野崇
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] ガーリック由来香気成分Dially1 trisulfideの白血病細胞における分子標的の解明2011

    • Author(s)
      須田峻
    • Organizer
      第65回日本栄養・食糧学会大会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Diallyl trisulfideによるチオレドキシンの修飾と抗がん作用ついて2011

    • Author(s)
      渡部和浩、須田峻、田中友紀、細野崇、関泰一郎、有賀豊彦
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Diallyl trisulfide(DATS)による白血病細胞のアポトーシス誘導について2011

    • Author(s)
      田中友紀、須田峻、細野崇、関泰一郎、有賀豊彦
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Dially1 trisulfideによるチオレドキシンの修飾と抗がん作用ついて2011

    • Author(s)
      渡部和浩
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dially1 trisulfideによる白血病細胞のアポトーシス誘導について2011

    • Author(s)
      田中友紀
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Diallyl trisulfide(DATS)による白血病細胞のアポトーシス誘導について2011

    • Author(s)
      田中友紀
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Diallyl trisulfideによるチオレドキシンの修飾と抗がん作用ついて2011

    • Author(s)
      渡部和浩
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Alk(en) yl trisulfideの側鎖構造と大腸がん細胞株の増殖抑制活性との関係について2010

    • Author(s)
      田中友紀、飯塚裕司、須田峻、細野崇、関泰一郎、有賀豊彦
    • Organizer
      第25回日本香辛料研究会学術講演会
    • Place of Presentation
      静岡
    • Year and Date
      2010-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Alk(en)yl trisulfideの側鎖構造と大腸がん細胞株の増殖抑制活性との関係について2010

    • Author(s)
      田中友紀
    • Organizer
      第25回日本香辛料研究会学術講演会
    • Place of Presentation
      静岡
    • Year and Date
      2010-11-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト白血病細胞に対するガーリック由来香気成分diallyl trisulfideの抗がん作用について2010

    • Author(s)
      田中友紀、稲田薫、関口雄亮、飯塚裕司、嶌本麻衣子、須田峻、細野崇、関泰一郎、有賀豊彦
    • Organizer
      64回日本栄養・食糧学会大会
    • Place of Presentation
      徳島
    • Year and Date
      2010-05-23
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト白血病細胞に対するガーリック由来香気成分diallyl trisulfideの抗がん作用について2010

    • Author(s)
      田中友紀
    • Organizer
      第64回 日本栄養・食糧学会大会
    • Place of Presentation
      徳島
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] ガーリック由来香気成分diallyl trisulfideによる各種白血病細胞株のアポトーシス誘導について2010

    • Author(s)
      嶌本麻衣子、稲田薫、田中理江、杉原明日香、飯塚裕司、田中友紀、細野崇、関泰一郎、有賀豊彦
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Alk(en) yl trisulfideの側鎖構造と抗がん活性との関係について2010

    • Author(s)
      飯塚裕司、嶌本麻衣子、田中友紀、須田峻、関口雄亮、細野崇、関泰一郎、有賀豊彦
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Phytochemical の機能性とその作用メカニズムーガーリック由来の有機硫黄化合物の機能性を中心として2009

    • Author(s)
      関泰一郎
    • Organizer
      第147回日本獣医学会教育講演
    • Place of Presentation
      宇都宮
    • Year and Date
      2009-04-04
    • Related Report
      2009 Annual Research Report
  • [Book] Allium : Garlic and Onion(IV. Biological Functionality of Herbs and Spices) The Basics of Functional Foods2012

    • Author(s)
      Seki T, Hosono T, Ariga T.
    • Publisher
      DEStech Publications(in press)
    • Related Report
      2011 Final Research Report
  • [Book] スパイス・ハーブの機能と最新応用技術(ニンニクの機能 pp160-164)2011

    • Author(s)
      関泰一郎
    • Total Pages
      288
    • Publisher
      シーエムシー(東京)
    • Related Report
      2010 Annual Research Report
  • [Book] ニンニクの機能スパイス・ハーブの機能性と最新応用技術2010

    • Author(s)
      関泰一郎、細野崇、有賀豊彦、中谷延二
    • Publisher
      シー・エムシー出版
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://hp.brs.nihon-u.ac.jp/~eiyo/

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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