Elucidation of functionality of equol-producing bacteria

• Project/Area Number: 21580168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: TAMURA Motoi 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品機能研究領域, 上席研究員 (70353943)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Hiroyuki 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品安全研究領域, 主任研究員 (30308192)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エコール / 腸内フローラ / ダイゼイン / エストロゲン / マウス / β-エストロラジオール / エストロン / 脂質代謝 / βエストラジオール

Research Abstract

Equol is a metabolite of daidzein that is produced by the intestinal flora in the gut. Life style disease prevention of equol has been expected. In our research, In in vitro anaerobic incubation of equol-producing bacteria with estriol, estrone, andβ-estradiol, we firstly elucidated that equol-producing bacteria strongly converted estrone toβ-estradiol. Administration of equol-producing bacteria to mice fed the 0.05%-daidzein-10% lard diet affected the lipid metabolism and fecal bile acid concentration. The urinary amounts ofβ-estradiol were significantly lower in the mice administrated of equol-producing bacteria than in the mice administrated of physiological saline.

Research Products

• [Journal Article] Lactobacillus rhamnosus JCM 2771 : Impact on metabolism of isoflavonoids in the fecal flora from a male equol producer (2011)
  • Author(s): Tamura M, Hori S, Nakagawa H.
  • Journal Title: Curr Microbiol Volume: 62(5) Pages: 1632-1637
  • URL: http://www.springerlink.com/content/6q6607211103677m/?MUD=MP
  • Peer Reviewed
• [Journal Article] Apple pectin affects the efficacy of epigallocatechin gallate on oral sucrose tolerance test in adult mice (2011)
  • Author(s): Tamura M, Hori S.
  • Journal Title: Int J Vitam Nutr Res Volume: 81(6) Pages: 372-377
  • Peer Reviewed
• [Journal Article] Lactobacillus rhamnosus JCM2771 : Impact on metabolism of isoflavonoids in the fecal flora from a male equol producer. (2011)
  • Author(s): Tamura M, Hori S, Nakagawa H.
  • Journal Title: Current Microbiology Volume: 62 Pages: 1632-1637
  • Peer Reviewed
• [Journal Article] バイオジェニックス (2010)
  • Author(s): 田村基
  • Journal Title: 日本食品科学工学会 Volume: 57(10) Pages: 446-446
  • NAID: 130000442563
  • Peer Reviewed
• [Journal Article] エクオール(equol) (2010)
  • Author(s): 田村基
  • Journal Title: 日本食品科学工学会誌 Volume: 57(11) Pages: 492-493
  • Peer Reviewed
• [Journal Article] Lactobacillus collinoides JCM1123^T : effects on mouse plasma cholesterol and isoflavonoids in the caecum (2009)
  • Author(s): Tamura M, Hori S, Nakagawa H.
  • Journal Title: Antonie van Leeuwenhoek Volume: 96(4) Pages: 621-626
  • URL: http://www.springerlink.com/content/w261261n133n3241/?MUD=MP
  • Peer Reviewed
• [Journal Article] Lactobacillus collinoides JCM1123T : effects on mouse plasma cholesterol and isoflavonoids in the caecum. (2009)
  • Author(s): Tamura M, Hori S, Nakagawa H.
  • Journal Title: Antonie van Leeuwenhoek 96 Pages: 621-626
  • Peer Reviewed
• [Presentation] Impact of dihydrodaidzein-producing Clostridium-like intestinal bacterium, strain TM-40 on in vitro metabolism of daidzein by equol-producing bacterium and fecal microbiota from human (2011)
  • Author(s): Tamura M, Hori S, Kurusu Y, Nakagawa H
  • Organizer: The Joint Meeting of the 17th International Symposium on Gnotobiology(ISG) and the 34th Congress of Society for Microbial Ecology and Disease(SOMED)
  • Place of Presentation: ナビオス横浜
  • Year and Date: 2011-11-23
• [Presentation] Impact of dihydrodaidzein-producing Clostridium-like intestinal bacterium, strain TM-40 on in vitro metabolism of daidzein by equol-producing bacterium and fecal microbiota from human (2011)
  • Author(s): 田村基、堀幸子、久留主幸江、中川博之
  • Organizer: The Joint Meeting of the 17th International Symposium on Gnotobiology (ISG) and the 34th Congress of Society for Microbial Ecology and Disease (SOMED)
  • Place of Presentation: ナビオス横浜(神奈川県)
  • Year and Date: 2011-11-23
• [Presentation] イソフラボン代謝と腸内細菌について (2010)
  • Author(s): 田村基
  • Organizer: Soy Nutrition Institute Japan第4回学術総会
  • Place of Presentation: (独)国立健康・栄養研究所
  • Year and Date: 2010-02-19
• [Book] 免疫機能性食品の基礎と応用 (2010)
  • Author(s): 田村基、伊藤喜久治
  • Total Pages: 11
  • Publisher: 腸内フローラとその食物による変動シーエムシー出版
• [Book] 免疫機能性食品の基礎と応用 (2010)
  • Author(s): 田村基、伊藤喜久治
  • Total Pages: 11
  • Publisher: シーエムシー出版