| Project/Area Number |
21580227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Nagasaki University |
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Principal Investigator |
SAKAKURA Yoshitaka 長崎大学, 大学院・水産・環境科学総合研究科, 教授 (20325682)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Hideki 独立行政法人水産総合研究センター, 瀬戸内海区水産研究所, 主任技術開発員 (10466261)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKATANI Tomohiro 長崎大学, 大学院・水産・環境科学総合研究科, 准教授 (90304972)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 生理 / 行動 / トラフグ / テトロドトキシン / 脳 / 組織学 / 情動 |
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| Research Abstract |
We investigated the effects of tetrodotoxin(TTX) on the central nervous system and behavior of juvenile tiger puffer Takifugu rubripes. We compared distribution of TTX in the tissues between wild and artificially raised fish. Monoclonal anti-TTX antibody was used for detection of TTX in the various tissues of tiger puffer juveniles. We detected TTX in the skin and liver of the wild puffer juveniles. Moreover, we firstly demonstrated that TTX were present in the central nervous system, especially in the hindbrain, cerebellum, optic nerve and olfactory epithelium. When TTX was administrated to artificially raised puffer juveniles by diet and injection to muscle, the same TTX distribution as wild fish was observed. Behavioral observation revealed that artificially raised puffer juveniles, which do not contain TTX, were bold to a novel environment comparing to the wild juveniles. Administration of TTX to the juvenile puffer significantly decreased boldness and agonistic interactions, and raised the survival after release to the environment with predators. Synthesizing these results, we hypothesized that TTX is a kind of neuro-transmitter.
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