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Risk assessments of the emerging fish pathogen in the environmental condition of rising water temperature.

Research Project

Project/Area Number 21580229
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionUniversity of Miyazaki

Principal Investigator

YOSHIDA Terutoyo  宮崎大学, 農学部, 准教授 (20240294)

Co-Investigator(Kenkyū-buntansha) ITAMI Toshiaki  宮崎大学, 農学部, 教授 (00363573)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsC群レンサ球菌 / Streptococcus dysgalactiae / 高水温 / ブリ / カンパチ / 魚病 / 抗原 / 薬剤感受性 / レンサ球菌 / ブリ属魚類 / streptococcus dysgalactiae / ランスフィールドC群 / 抗原型 / コンゴーレッド
Research Abstract

TH-CR(Todd-Hewitt Congo red) agar was developed to detect and presumptively identify the GCSD(Group C Streptococcus dysgalactiae) obtained from artificially or naturally infected fish. Typical symptoms were observed in artificially infected fish at higher temperature(28°C), thus need an attention at the high water temperature season. The genetic analysis revealed that the fish isolates were genetically very similar to each other with high DNA. DNA relatedness and sequence homology. Seventeen isolates in GCSD collected Asian countries were found to be resistant to oxytetracycline and carried the tet(M) gene, except for the strains collected in Taiwan and the PP1564 strain collected in China. The amino acid sequence of SOF was identical among fish isolates. A primer set targeting the sof-FD gene was designed and applied to a PCR assay for discriminating fish isolates from mammalian isolates.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (15 results)

All 2011 2010 2009

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (1 results)

  • [Journal Article] Infective endocarditis with Lactococcus garvieae in Japan2011

    • Author(s)
      Y. Watanabe, T. Naito, K. Kikuchi, Y. Amari, Y. Uehara, H. Isonuma, H. Hisaoka, T. Yoshida, K. Yaginuma, N. Takaya, H. Daida and K. Hiramatsu
    • Journal Title

      Journal of Medical Case Reports

      Volume: 5 Pages: 356-359

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and expression of serum opacity factor in fish pathogenic Streptococcus dysgalactiae and its application to discriminate between fish and mammalian isolates2011

    • Author(s)
      Nishiki, Y. Horikiri, T. Itami and T. Yoshida
    • Journal Title

      FEMS Microbiology Letter

      Volume: 323 Pages: 68-74

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epidemiological study on Lactococcus garvieae isolates from fish in Japan Fisheries Science2011

    • Author(s)
      I. NIshiki, M. Furukawa, S. Matsui, T. Itami, T. Nakai and T. Yoshida
    • Journal Title

      Fisheries Science

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and expression of serum opacity factor in fish pathogenic Streptococcus dysgalactiae and its application to discriminate between fish and mammalian isolates2011

    • Author(s)
      Issei Nishi, et al
    • Journal Title

      FEMS Microbiology Letters

      Volume: 323 Issue: 1 Pages: 68-74

    • DOI

      10.1111/j.1574-6968.2011.02360.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissemination of streptococcal pyrogenetic exotoxin G(spegg) with an IS-like element in fish isolates of Streptococcus dysgalactiae2010

    • Author(s)
      M. Abdelsalam, S. C. Chen and T. Yoshida
    • Journal Title

      FEMS Microbiology Letters

      Volume: 309 Pages: 105-113

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Homogeneity of Streptococcus dysgalactiae isolated from amberjack, Seriola dumerili, in Japan2010

    • Author(s)
      I. Nishiki, M. Noda, T. Itami and T. Yoshida
    • Journal Title

      Fisheries Science

      Volume: 76 Pages: 661-668

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic and genetic characterizations of Streptococcus dysgalactiae strains from fish collected in Japan and other Asian countries2010

    • Author(s)
      M. Abdelsalam, S. C. Chen and T. Yoshida
    • Journal Title

      FEMS Microbiology Letters

      Volume: 302 Pages: 32-38

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Homogeneity of Streptococcus dysgalactiae from farmed amberjack Seriola dumerili in Japan2010

    • Author(s)
      I.Nishiki, M.Noda, T.Itami, T.Yoshida
    • Journal Title

      Fisheries Science

      Volume: 76 Pages: 661-668

    • NAID

      10026551523

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic and genetic characterization of Streptococcus dysgalactiae strains isolated from fish collected in Japan and other Asian countries2010

    • Author(s)
      M.Abdelsalam, S.C.Chen, T.Yoshida
    • Journal Title

      FEMS Microbiology Letters

      Volume: 302 Pages: 32-38

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissemination of streptococcal pyrogenic exotoxin G (spegg) with an IS-like element in fish isolates of Streptococcus dysgalactiae2010

    • Author(s)
      M.Abdelsalam, S.C.Chen, T.Yoshida
    • Journal Title

      FEMS Microbiology Letters

      Volume: 309 Pages: 105-113

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic and genetic characterization of Streptococcus dysgalactiae strains isolated from fish collected in Japan and other Asian countries2010

    • Author(s)
      M.Abdelsalam, S.C.Chen, T.Yoshida
    • Journal Title

      FEMS Microbiology Letters 302

      Pages: 32-38

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surface properties of Streptococcus dysgalactiae strains isolated from marine fish Bull.2009

    • Author(s)
      M. Abdelsalam, S. C. Chen and T. Yoshida
    • Journal Title

      Fish Pathol

      Volume: 29-1 Pages: 16-24

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] ブリ属魚類由来Streptococcus dysgalactiaeの薬剤感受性2009

    • Author(s)
      西木一生、野本竜平、米村輝一朗、中西健二、平江多績、村瀬拓也、伊丹利明、吉田照豊
    • Journal Title

      日本水産学会誌

      Volume: 75-3 Pages: 451-452

    • NAID

      10024975246

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of Congo red agar to detect Streptococcus dysgalactiae from diseased fish.2009

    • Author(s)
      M. Abdelsalam, K. Nakanishi, K. Yonemura., . T. Itami, S. C. Chen and T. Yoshida
    • Journal Title

      Journal of Applied Ichthyology

      Volume: 25 Pages: 442-446

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] 魚類由来C群 Streptococcus dysgalactiae の分類学的研究2009

    • Author(s)
      野田真広, 西木一生, 吉田照豊
    • Organizer
      第18回 Lancefield レンサ球菌研究会
    • Place of Presentation
      九州大学医系キャンパス
    • Year and Date
      2009-06-26
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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