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Virus detection and prevention of diseases brought by black tiger prawn migrating toward north with global warming

Research Project

Project/Area Number 21580230
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionUniversity of Miyazaki

Principal Investigator

ITAMI Toshiaki  宮崎大学, 農学部, 教授 (00363573)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Terutoyo  宮崎大学, 農学部, 准教授 (20240294)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords魚病 / クルマエビ / ウシエビ / ウイルス / 検出 / RNAi / 特定疾病 / LAMP法 / 定量LAMP法
Research Abstract

Expanding the global warming, black tiger prawn, Penaeus monodon, has been migrating toward the north. In Hyuganada sea are, native species of kuruma prawn, Marsupenaeus japonicas, and up-coming tiger prawn are occupying the same habitat. In order to detect the virus that might have been brought by black tiger prawn, a sensitive detection method has been established. Cutting-edge technologies for preventing diseases have been attempted.
1. Pathogenic viruses have not been detected in the black tiger prawn found in Hyuganada sea are using real-time LAMP method.
2. Any virus-positive black tiger prawn was not found along the Miyazaki cost line.
3. Shrimp showed the resistance against WSSV infection when caspase gene was knocked down.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (38 results)

All 2012 2011 2010 2009 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (17 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Journal Article] Molecular cloning and characterization of the NADPH oxidase from the kuruma shrimp, Marsupenaeus japonicus : Early gene up-regulation after Vibrio penaeicida and poly (I:C) stimulations in vitro2012

    • Author(s)
      Mari Inada
    • Journal Title

      Molecular and Cellular Probes

      Volume: 26巻1号 Issue: 1 Pages: 29-41

    • DOI

      10.1016/j.mcp.2011.11.002

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Class B scavenger receptor, Croquemort from kuruma shrimp Marsupenaeus japonicus2011

    • Author(s)
      Mekata, T., Okugawa, S., Inada, M., Yoshimine, M., Nishi, J., Kono, T., Sakai, M., Sudhakaran, R, Itami, T
    • Journal Title

      Molecular cloning and characterization

      Volume: 25(2-3) Pages: 94-100

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Class B scavenger receptor, Croquemort from kuruma shrimp Marsupenaeus japonicus : Molecular cloning and characterization2011

    • Author(s)
      Mekata, T.
    • Journal Title

      Molecular and Cellular Probes

      Volume: 25 Issue: 2-3 Pages: 94-100

    • DOI

      10.1016/j.mcp.2011.02.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deciphering the DNA repair protein, Rad23 from kuruma shrimp Marsupenaeus japonicus : full-length cDNA cloning and characterization2011

    • Author(s)
      Sudhakaran, R.
    • Journal Title

      Letters in Applied Microbiology

      Volume: 53 Issue: 1 Pages: 63-72

    • DOI

      10.1111/j.1472-765x.2011.03073.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The concentration of white spot disease virus for its detection in sea water usinga combined ferric colloid adsorption- and foam separation-based met2011

    • Author(s)
      Suzuki, Y.
    • Journal Title

      Journal of Virological Method

      Volume: 173 Issue: 2 Pages: 227-232

    • DOI

      10.1016/j.jviromet.2011.02.010

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp Marsupenaeus japonicus2010

    • Author(s)
      Mekata, T., Sudhakaran, R., Okugawa, S., Inada, M., Kono, T., Sakai, M., Itami, T
    • Journal Title

      Fish and Shellfish Immunology

      Volume: 28-2 Pages: 571-578

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and characterization of the nitric oxide synthase gene from kuruma shrimp Marsupenaeus japonicus2010

    • Author(s)
      Inada, M., Mekata, T., Sudhakaran, R., Okugawa, S., Kono, T., El Asely, A. M., Linh, N., Yoshida, T., Sakai, M., Yui, T., Itami, T.
    • Journal Title

      Fish and Shellfish Immunology

      Volume: 28-3 Pages: 701-710

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and transcriptional analysis of a newly identified anti-lipopolysaccharide factor gene in kuruma shrimp, Marsupenaeus japonicus2010

    • Author(s)
      Mekata, T., Sudhakaran, R., Okugawa, S., Kono, T., Sakai, M., Itami, T
    • Journal Title

      Letters in Applied Microbiology

      Volume: 50-1 Pages: 112-119

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Double-stranded RNA-mediated silencing of the white spot syndrome virus VP28 gene in kuruma shrimp, Marsupenaeus japonicas2010

    • Author(s)
      R.Sudhakaran
    • Journal Title

      Aquaculture Research

      Volume: 1 Pages: 1-10

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Class B scavenger receptor, Croquemort from kuruma shrimp Marsupenaeus japonicus Molecular cloning and characterization2010

    • Author(s)
      T.Mekata
    • Journal Title

      Molecular & Cellular Probes

      Volume: 25 Pages: 94-100

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp, Marsupenaeus japonicus2010

    • Author(s)
      T.Mekata
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 28 Pages: 571-578

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and transcriptional analysis of a newly identified anti-lipopolysaccharide factor gene in kuruma shrimp, Marsupenaeus japonicus2010

    • Author(s)
      T.Mekata
    • Journal Title

      Letters in Applied Microbiology 50

      Pages: 112-119

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time transcription loop-mediated isothermal amplification for rapid detection of yellow head virus in shrimp2009

    • Author(s)
      Mekata, T., Sudhakaran, R., Kono, T., U-taynapun, K., Supamattaya, K., Suzuki, Y., Sakai, M., Itami, T
    • Journal Title

      Journal of Virological Methods

      Volume: 162-1 Pages: 81-87

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of yellow head virus in shrimp2009

    • Author(s)
      T.Mekata
    • Journal Title

      Journal of Virological Methods 162

      Pages: 81-87

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time quantitative LAMP(loop-mediated isothermal amplification)as a simple method for detecting white spot syndrome virus(WSSV)2009

    • Author(s)
      T.Mekata
    • Journal Title

      Letters in Applied Microbiology 48

      Pages: 25-32

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Shrimp Diseases and Prevention in Japan2011

    • Author(s)
      Itami, T
    • Organizer
      Miyazaki-PSU Joint Seminar
    • Place of Presentation
      タイ、ハジャイ市(招待講演)
    • Year and Date
      2011-12-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification and Expression Analysis of Toll interacting protein gene2011

    • Author(s)
      K. Kihara, M. Yoshimine, M. Inada, J. Nishi, T. Yoshida, T. Kono, M. Sakai and T. Itami
    • Organizer
      8th Symposium on Diseases in Asian Aquaculture
    • Place of Presentation
      Mangalore, India
    • Year and Date
      2011-11-23
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of cytochrome c in kuruma shrimp (Marsupenaeus japonicus)2011

    • Author(s)
      Yoshimine, M
    • Organizer
      8th Symposium on Diseases in Asian Aquaculture
    • Place of Presentation
      インド、マンガロール市
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular cloning, mRNA variants and NO generation of the Nitric Oxide Synthase Gene, MjNOS, in Kuruma Shrimp Marsupenaeus japonicas2011

    • Author(s)
      Inada, M
    • Organizer
      8th Symposium on Diseases in Asian Aquaculture
    • Place of Presentation
      インド、マンガロール市
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] cDNA cloning and expression analysis of astakine gene, MjAstaldne, in Kuruma Shrimp Marsupenaeus japonicus and change of hemocyte count on MjAstakine knockdown2011

    • Author(s)
      Inada, M
    • Organizer
      8th Symposium on Diseases in Asian Aquaculture
    • Place of Presentation
      インド、マンガロール市
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of Argonaute2 gene in kuruma shrimp (Marsupenaeus japonicus)2011

    • Author(s)
      Nishi, J
    • Organizer
      8th Symposium on Diseases in Asian Aquaculture
    • Place of Presentation
      インド、マンガロール市
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification and Expression Analysis of Astakine Gene2011

    • Author(s)
      M Inada, S Okugawa. J Nishi, M Yoshimine, R Sudhakaran, T Kono, T Mekata, T Yoshida, M Sakai and T Itami
    • Organizer
      Plant and Animal Genome XIX Conference
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2011-01-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification and Characterization of Dicer2 Gene in Kuruma Shrimp2010

    • Author(s)
      J Nishi, S Okugawa, M Inada, M Yoshimine, T Kono, T Mekata, T Yoshida, M Sakai, and T Itami
    • Organizer
      The 39th Scientific Symposium of UJNR Aquaculture Panel
    • Place of Presentation
      Kagoshima, Japan
    • Year and Date
      2010-10-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Molecular Cloning and Expression Analyses of Astakine Gene, MjAstakine, in Kuruma Shrimp Marsupenaeus japonicus2010

    • Author(s)
      M.Inada
    • Organizer
      6th International Symposium on Aquatic Animal Health
    • Place of Presentation
      Tampa, USA
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] A Newly Identified Anti-lipopolysaccharide Factor (ALF2) Gene in Kurama Shrimp Marsupenaeus japonicus2010

    • Author(s)
      S Okugawa
    • Organizer
      6th International Symposium on Aquatic Animal Health
    • Place of Presentation
      Tampa, USA
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular Cloning and Analysis of c-Jun N-Terminal Kinase (JNK) in Kuruma Shrimp Marsupenaeus japonicus2010

    • Author(s)
      M Yoshimine
    • Organizer
      6th International Symposium on Aquatic Animal Health
    • Place of Presentation
      Tampa, USA
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development and Evaluation of Real-time Loop-mediated Isothermal Amplifications Assay for Detection of Disease Viruses in Penaeid Shrimp2010

    • Author(s)
      T Itami, T Mekata, R Sudhakaran, T Kono, T Yoshida, Y Suzuki, K Supamattaya M Inada, S Okugawa, J Nishi, M Yoshimine and M Sakai
    • Organizer
      6th International Symposium on Aquatic Animal Health
    • Place of Presentation
      Tampa, USA
    • Year and Date
      2010-09-07
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Characterization of Tumor Necrosis Factor Ligand Superfamily Gene in Kuruma Shrimp2010

    • Author(s)
      S Okugawa, R Sudhakaran, M Inada, T Mekata, J Nishi, M Yoshimine, T Yoshida, Y Suzuki, T Kono, M Sakai and T Itami
    • Organizer
      6th International Symposium on Aquatic Animal Health
    • Place of Presentation
      Tampa, USA
    • Year and Date
      2010-09-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Application of Real-Time Loop-Mediated Isothermal Amplification Assay for Detection of Penaeid Shrimp Viruses2010

    • Author(s)
      T.Itami
    • Organizer
      JSPS-NRCT International Symposium 2010
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2010-02-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development and Evaluation of Real-Time Loop-Mediated Isothermal Amplification for Penaeid Viruses2009

    • Author(s)
      T.Itami
    • Organizer
      Integrated Technologies for Advanced Shrimp Production
    • Place of Presentation
      Oceanic Institute(Hawaii, USA)
    • Year and Date
      2009-10-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Characterization of Anti-Lipopolysaccharide Factors in Kuruma Shrimp2009

    • Author(s)
      S.Okugawa
    • Organizer
      Integrated Technologies for Advanced Shrimp Production
    • Place of Presentation
      Oceanic Institute(Hawaii, USA)
    • Year and Date
      2009-10-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Characterization of anti-lipopolysaccharide factors, MjALF/ALF2, in Kuruma shrimp Marsupenaeus japonicus2009

    • Author(s)
      T.Mekata
    • Organizer
      11th International Congress ISDCI
    • Place of Presentation
      プラハ(チェコ共和国)
    • Year and Date
      2009-06-10
    • Related Report
      2009 Annual Research Report
  • [Book] 第3章ウイルス病§6甲殻類などにおけるウイルス病、「改定・魚病学概論」2012

    • Author(s)
      伊丹利明
    • Publisher
      恒星社厚生閣(東京)
    • Related Report
      2011 Final Research Report
  • [Book] 魚病学概論(改訂第二版)2012

    • Author(s)
      伊丹利明
    • Total Pages
      192
    • Publisher
      恒星社厚生閣
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] LAMP法による急性ウイルス血症原因ウイルスの検出方法及び検出試薬キット2011

    • Inventor(s)
      伊丹利明,酒井正博,河野智哉,国立大学法人宮崎大学
    • Industrial Property Rights Holder
      国立大学法人宮崎大学
    • Industrial Property Number
      2011-050110
    • Filing Date
      2011-03-08
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 甲殻類急性ウイルス血症に対するワクチン2010

    • Inventor(s)
      酒井正博,伊丹利明,河野智哉,国立大学法人宮崎大学
    • Industrial Property Rights Holder
      国立大学法人宮崎大学
    • Filing Date
      2010-06-22
    • Related Report
      2011 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 甲殻類急性ウイルス血症に対するワクチン2009

    • Inventor(s)
      酒井正博,伊丹利明,河野智哉,国立大学法人宮崎大学
    • Industrial Property Rights Holder
      国立大学法人宮崎大学
    • Industrial Property Number
      2009-162802
    • Filing Date
      2009-07-09
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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