Virus detection and prevention of diseases brought by black tiger prawn migrating toward north with global warming

• Project/Area Number: 21580230
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: University of Miyazaki
• Principal Investigator: ITAMI Toshiaki 宮崎大学, 農学部, 教授 (00363573)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Terutoyo 宮崎大学, 農学部, 准教授 (20240294)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 魚病 / クルマエビ / ウシエビ / ウイルス / 検出 / RNAi / 特定疾病 / LAMP法 / 定量LAMP法

Research Abstract

Expanding the global warming, black tiger prawn, Penaeus monodon, has been migrating toward the north. In Hyuganada sea are, native species of kuruma prawn, Marsupenaeus japonicas, and up-coming tiger prawn are occupying the same habitat. In order to detect the virus that might have been brought by black tiger prawn, a sensitive detection method has been established. Cutting-edge technologies for preventing diseases have been attempted.
1. Pathogenic viruses have not been detected in the black tiger prawn found in Hyuganada sea are using real-time LAMP method.
2. Any virus-positive black tiger prawn was not found along the Miyazaki cost line.
3. Shrimp showed the resistance against WSSV infection when caspase gene was knocked down.

Research Products

• [Journal Article] Molecular cloning and characterization of the NADPH oxidase from the kuruma shrimp, Marsupenaeus japonicus : Early gene up-regulation after Vibrio penaeicida and poly (I:C) stimulations in vitro (2012)
  • Author(s): Mari Inada
  • Journal Title: Molecular and Cellular Probes Volume: 26巻1号 Issue: 1 Pages: 29-41
  • DOI: 10.1016/j.mcp.2011.11.002
  • Peer Reviewed
• [Journal Article] Class B scavenger receptor, Croquemort from kuruma shrimp Marsupenaeus japonicus (2011)
  • Author(s): Mekata, T., Okugawa, S., Inada, M., Yoshimine, M., Nishi, J., Kono, T., Sakai, M., Sudhakaran, R, Itami, T
  • Journal Title: Molecular cloning and characterization Volume: 25(2-3) Pages: 94-100
  • Peer Reviewed
• [Journal Article] Class B scavenger receptor, Croquemort from kuruma shrimp Marsupenaeus japonicus : Molecular cloning and characterization (2011)
  • Author(s): Mekata, T.
  • Journal Title: Molecular and Cellular Probes Volume: 25 Issue: 2-3 Pages: 94-100
  • DOI: 10.1016/j.mcp.2011.02.001
  • Peer Reviewed
• [Journal Article] Deciphering the DNA repair protein, Rad23 from kuruma shrimp Marsupenaeus japonicus : full-length cDNA cloning and characterization (2011)
  • Author(s): Sudhakaran, R.
  • Journal Title: Letters in Applied Microbiology Volume: 53 Issue: 1 Pages: 63-72
  • DOI: 10.1111/j.1472-765x.2011.03073.x
  • Peer Reviewed
• [Journal Article] The concentration of white spot disease virus for its detection in sea water usinga combined ferric colloid adsorption- and foam separation-based met (2011)
  • Author(s): Suzuki, Y.
  • Journal Title: Journal of Virological Method Volume: 173 Issue: 2 Pages: 227-232
  • DOI: 10.1016/j.jviromet.2011.02.010
  • Peer Reviewed
• [Journal Article] A novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp Marsupenaeus japonicus (2010)
  • Author(s): Mekata, T., Sudhakaran, R., Okugawa, S., Inada, M., Kono, T., Sakai, M., Itami, T
  • Journal Title: Fish and Shellfish Immunology Volume: 28-2 Pages: 571-578
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of the nitric oxide synthase gene from kuruma shrimp Marsupenaeus japonicus (2010)
  • Author(s): Inada, M., Mekata, T., Sudhakaran, R., Okugawa, S., Kono, T., El Asely, A. M., Linh, N., Yoshida, T., Sakai, M., Yui, T., Itami, T.
  • Journal Title: Fish and Shellfish Immunology Volume: 28-3 Pages: 701-710
  • Peer Reviewed
• [Journal Article] Molecular cloning and transcriptional analysis of a newly identified anti-lipopolysaccharide factor gene in kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): Mekata, T., Sudhakaran, R., Okugawa, S., Kono, T., Sakai, M., Itami, T
  • Journal Title: Letters in Applied Microbiology Volume: 50-1 Pages: 112-119
  • Peer Reviewed
• [Journal Article] Double-stranded RNA-mediated silencing of the white spot syndrome virus VP28 gene in kuruma shrimp, Marsupenaeus japonicas (2010)
  • Author(s): R.Sudhakaran
  • Journal Title: Aquaculture Research Volume: 1 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Class B scavenger receptor, Croquemort from kuruma shrimp Marsupenaeus japonicus Molecular cloning and characterization (2010)
  • Author(s): T.Mekata
  • Journal Title: Molecular & Cellular Probes Volume: 25 Pages: 94-100
  • Peer Reviewed
• [Journal Article] A novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): T.Mekata
  • Journal Title: Fish & Shellfish Immunology Volume: 28 Pages: 571-578
  • Peer Reviewed
• [Journal Article] Molecular cloning and transcriptional analysis of a newly identified anti-lipopolysaccharide factor gene in kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): T.Mekata
  • Journal Title: Letters in Applied Microbiology 50 Pages: 112-119
  • Peer Reviewed
• [Journal Article] Real-time transcription loop-mediated isothermal amplification for rapid detection of yellow head virus in shrimp (2009)
  • Author(s): Mekata, T., Sudhakaran, R., Kono, T., U-taynapun, K., Supamattaya, K., Suzuki, Y., Sakai, M., Itami, T
  • Journal Title: Journal of Virological Methods Volume: 162-1 Pages: 81-87
  • Peer Reviewed
• [Journal Article] Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of yellow head virus in shrimp (2009)
  • Author(s): T.Mekata
  • Journal Title: Journal of Virological Methods 162 Pages: 81-87
  • Peer Reviewed
• [Journal Article] Real-time quantitative LAMP(loop-mediated isothermal amplification)as a simple method for detecting white spot syndrome virus(WSSV) (2009)
  • Author(s): T.Mekata
  • Journal Title: Letters in Applied Microbiology 48 Pages: 25-32
  • Peer Reviewed
• [Presentation] Shrimp Diseases and Prevention in Japan (2011)
  • Author(s): Itami, T
  • Organizer: Miyazaki-PSU Joint Seminar
  • Place of Presentation: タイ、ハジャイ市(招待講演)
  • Year and Date: 2011-12-12
• [Presentation] Identification and Expression Analysis of Toll interacting protein gene (2011)
  • Author(s): K. Kihara, M. Yoshimine, M. Inada, J. Nishi, T. Yoshida, T. Kono, M. Sakai and T. Itami
  • Organizer: 8th Symposium on Diseases in Asian Aquaculture
  • Place of Presentation: Mangalore, India
  • Year and Date: 2011-11-23
• [Presentation] Characterization of cytochrome c in kuruma shrimp (Marsupenaeus japonicus) (2011)
  • Author(s): Yoshimine, M
  • Organizer: 8th Symposium on Diseases in Asian Aquaculture
  • Place of Presentation: インド、マンガロール市
  • Year and Date: 2011-11-21
• [Presentation] Molecular cloning, mRNA variants and NO generation of the Nitric Oxide Synthase Gene, MjNOS, in Kuruma Shrimp Marsupenaeus japonicas (2011)
  • Author(s): Inada, M
  • Organizer: 8th Symposium on Diseases in Asian Aquaculture
  • Place of Presentation: インド、マンガロール市
  • Year and Date: 2011-11-21
• [Presentation] cDNA cloning and expression analysis of astakine gene, MjAstaldne, in Kuruma Shrimp Marsupenaeus japonicus and change of hemocyte count on MjAstakine knockdown (2011)
  • Author(s): Inada, M
  • Organizer: 8th Symposium on Diseases in Asian Aquaculture
  • Place of Presentation: インド、マンガロール市
  • Year and Date: 2011-11-21
• [Presentation] Characterization of Argonaute2 gene in kuruma shrimp (Marsupenaeus japonicus) (2011)
  • Author(s): Nishi, J
  • Organizer: 8th Symposium on Diseases in Asian Aquaculture
  • Place of Presentation: インド、マンガロール市
  • Year and Date: 2011-11-21
• [Presentation] Identification and Expression Analysis of Astakine Gene (2011)
  • Author(s): M Inada, S Okugawa. J Nishi, M Yoshimine, R Sudhakaran, T Kono, T Mekata, T Yoshida, M Sakai and T Itami
  • Organizer: Plant and Animal Genome XIX Conference
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-01-19
• [Presentation] Identification and Characterization of Dicer2 Gene in Kuruma Shrimp (2010)
  • Author(s): J Nishi, S Okugawa, M Inada, M Yoshimine, T Kono, T Mekata, T Yoshida, M Sakai, and T Itami
  • Organizer: The 39th Scientific Symposium of UJNR Aquaculture Panel
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2010-10-26
• [Presentation] Molecular Cloning and Expression Analyses of Astakine Gene, MjAstakine, in Kuruma Shrimp Marsupenaeus japonicus (2010)
  • Author(s): M.Inada
  • Organizer: 6th International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, USA
  • Year and Date: 2010-09-15
• [Presentation] A Newly Identified Anti-lipopolysaccharide Factor (ALF2) Gene in Kurama Shrimp Marsupenaeus japonicus (2010)
  • Author(s): S Okugawa
  • Organizer: 6th International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, USA
  • Year and Date: 2010-09-15
• [Presentation] Molecular Cloning and Analysis of c-Jun N-Terminal Kinase (JNK) in Kuruma Shrimp Marsupenaeus japonicus (2010)
  • Author(s): M Yoshimine
  • Organizer: 6th International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, USA
  • Year and Date: 2010-09-15
• [Presentation] Development and Evaluation of Real-time Loop-mediated Isothermal Amplifications Assay for Detection of Disease Viruses in Penaeid Shrimp (2010)
  • Author(s): T Itami, T Mekata, R Sudhakaran, T Kono, T Yoshida, Y Suzuki, K Supamattaya M Inada, S Okugawa, J Nishi, M Yoshimine and M Sakai
  • Organizer: 6th International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, USA
  • Year and Date: 2010-09-07
• [Presentation] Characterization of Tumor Necrosis Factor Ligand Superfamily Gene in Kuruma Shrimp (2010)
  • Author(s): S Okugawa, R Sudhakaran, M Inada, T Mekata, J Nishi, M Yoshimine, T Yoshida, Y Suzuki, T Kono, M Sakai and T Itami
  • Organizer: 6th International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, USA
  • Year and Date: 2010-09-07
• [Presentation] Application of Real-Time Loop-Mediated Isothermal Amplification Assay for Detection of Penaeid Shrimp Viruses (2010)
  • Author(s): T.Itami
  • Organizer: JSPS-NRCT International Symposium 2010
  • Place of Presentation: 東京海洋大学(東京)
  • Year and Date: 2010-02-27
• [Presentation] Development and Evaluation of Real-Time Loop-Mediated Isothermal Amplification for Penaeid Viruses (2009)
  • Author(s): T.Itami
  • Organizer: Integrated Technologies for Advanced Shrimp Production
  • Place of Presentation: Oceanic Institute(Hawaii, USA)
  • Year and Date: 2009-10-14
• [Presentation] Characterization of Anti-Lipopolysaccharide Factors in Kuruma Shrimp (2009)
  • Author(s): S.Okugawa
  • Organizer: Integrated Technologies for Advanced Shrimp Production
  • Place of Presentation: Oceanic Institute(Hawaii, USA)
  • Year and Date: 2009-10-14
• [Presentation] Characterization of anti-lipopolysaccharide factors, MjALF/ALF2, in Kuruma shrimp Marsupenaeus japonicus (2009)
  • Author(s): T.Mekata
  • Organizer: 11th International Congress ISDCI
  • Place of Presentation: プラハ(チェコ共和国)
  • Year and Date: 2009-06-10
• [Book] 第3章ウイルス病§6甲殻類などにおけるウイルス病、「改定・魚病学概論」 (2012)
  • Author(s): 伊丹利明
  • Publisher: 恒星社厚生閣(東京)
• [Book] 魚病学概論(改訂第二版) (2012)
  • Author(s): 伊丹利明
  • Total Pages: 192
  • Publisher: 恒星社厚生閣
• [Remarks]
• [Patent(Industrial Property Rights)] LAMP法による急性ウイルス血症原因ウイルスの検出方法及び検出試薬キット (2011)
  • Inventor(s): 伊丹利明,酒井正博,河野智哉,国立大学法人宮崎大学
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Number: 2011-050110
  • Filing Date: 2011-03-08
• [Patent(Industrial Property Rights)] 甲殻類急性ウイルス血症に対するワクチン (2010)
  • Inventor(s): 酒井正博,伊丹利明,河野智哉,国立大学法人宮崎大学
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Filing Date: 2010-06-22
  • Overseas
• [Patent(Industrial Property Rights)] 甲殻類急性ウイルス血症に対するワクチン (2009)
  • Inventor(s): 酒井正博,伊丹利明,河野智哉,国立大学法人宮崎大学
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Number: 2009-162802
  • Filing Date: 2009-07-09