| Project/Area Number |
21580232
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
KONDO Ryuji 福井県立大学, 海洋生物資源学部, 准教授 (30244528)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Shin-ichi 京都大学, 生態学研究センター, 教授 (50270723)
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| Co-Investigator(Renkei-kenkyūsha) |
KANEDA Atsushi 福井県立大学, 海洋生物資源学部, 講師 (70304649)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 微生物 / 環境微生物 / 光合成硫黄細菌 / 亜硫酸還元酵素遺伝子 / 有機汚濁 / 定量PCR / 密度成層湖 / 海底泥 / 亜硫酸還元酵素 / 紅色硫黄細菌 / 緑色硫黄細菌 / リアルタイムPCR |
|---|
| Research Abstract |
We developed new PCR primers for the rapid and sensitive detection and enumeration of dsrA in phototrophic purple and green sulfur bacteria simultaneously. The primers were applied to water and sediment samples from the Japanese meromictic Lake Suigetsu and the eutrophic Tokyo Bay, respectively. Phylogenetic analysis of the sequences of PCR products from lake water showed most clones were affiliated with brown-colored green sulfur bacteria within the Chlorobi, whileγ-proteobacterial species from sediments. Sequences of cloned PCR products show a differential distribution of sulfur bacterial groups in different environments.
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