| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Allergen analysis of major allergic yolk protein, β'-component (β'-c) was examined in this study and the results were as follows: (1) Five kinds of IgE sequential epitopes in β'-c were identified by overlapped peptide mapping and ELISA. The three kinds of the IgE-binding sites seem to be common epitopes of fish roe, because they were located in high homologous regions of fish β'-cs. (2) Six kinds of β'-c isoforms were prepared from mullet roe and they showed IgE cross-reactivity with salmon β'-c. (3) Vitellogenin subfragment over 1 μg/g were observed in matured salmon muscle and they showed β'-c-specific IgE-binding ability, suggesting food intake of fish meat may induce sensitization to fish roe allergy.
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