Elucidation of antigenic cross-reactivity mechanism in fish roe allergy
| Project/Area Number |
21580245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAEKI Hiroki 北海道大学, 大学院・水産科学研究院, 教授 (90250505)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Akihiko 北海道大学, 大学院・水産科学研究院, 特任教授 (40091483)
SHIMIZU Yutaka 北海道大学, 大学院・水産科学研究院, 技術職員 (00374629)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 魚卵 / 食物アレルギー / アレルゲン / β'-コンポーネント / ビテロジェニン / 魚肉 / 抗原交差性 / アレルギー / IgE結合部位 / イクラ / β'-component / カラスミ / β'-compornent |
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| Research Abstract |
Allergen analysis of major allergic yolk protein, β'-component (β'-c) was examined in this study and the results were as follows: (1) Five kinds of IgE sequential epitopes in β'-c were identified by overlapped peptide mapping and ELISA. The three kinds of the IgE-binding sites seem to be common epitopes of fish roe, because they were located in high homologous regions of fish β'-cs. (2) Six kinds of β'-c isoforms were prepared from mullet roe and they showed IgE cross-reactivity with salmon β'-c. (3) Vitellogenin subfragment over 1 μg/g were observed in matured salmon muscle and they showed β'-c-specific IgE-binding ability, suggesting food intake of fish meat may induce sensitization to fish roe allergy.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] シロザケ卵アレルゲンのエピトープ解析2011
Author(s)
清水裕, 岸村栄毅, 渡部一彦, 原彰彦, 佐伯宏樹
Organizer
平成23年度水産学会春期大会
Place of Presentation
東京都港区東京海洋大学品川キャンパス(震災により口頭発表は中止されたが、講演要旨集の発行をもって本大会における発表と取り扱うこととなった)
Year and Date
2011-03-28
Related Report
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