Probable tauropine-mediated interaction between marine redalgae and marine bacteria

• Project/Area Number: 21580253
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Kitasato University
• Principal Investigator: KAN-NO Nobuhiro 北里大学, 海洋生命科学部, 教授 (40169800)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 紅藻 / タウロピン / タウロピン脱水素酵素 / アミノ酸配列 / cDNA クローニング / Ahnfeltiopsis paradoxa / ハリガネ / 生理機能 / タウロピン資性化細菌 / 精製 / cDNAクローニング / 共生 / 相互作用 / 一次構造 / 生物相互作用

Research Abstract

To elucidate the physiological role of tauropine (rhodoic acid) which shows limited distribution among Gigartinales (Rhodophyta), the tauropin-mediated interaction between marine algae and marine bacteria was assumed and demonstrated. Tauropine dehydrogenases （ Ahp-TaDH1 and Ahp-TaDH2 ） were highly purified from Ahnfeltiopsis paradoxa.The catalytic properties and N-terminus and the partial amino acid sequences were determined. However, cloning of the tauropine dehydrogenase cDNA using partial amino acid sequence information was incomplete.