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Structure and function of crystalline chitin hydrolyzing chitinase from marine organisms

Research Project

Project/Area Number 21580254
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionNihon University

Principal Investigator

MATSUMIYA Masahiro  日本大学, 生物資源科学部, 教授 (60150702)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords代謝 / 酵素 / キチナーゼ / バイオマス / α-キチン / キチンオリゴ糖 / 基質分解特性 / N-アセチルグルコサミン / 遺伝子 / クローニング
Research Abstract

Crystalline chitin is a major structural structure component of arthropods. We purified several chitinases from marine organisms which take the arthropods as food and investigated the enzymatic properties and reaction. We also obtained several cDNA clones of the chitinases from marine organisms and investigated the structure and function of the chitinases.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (51 results)

All 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 3 results) Presentation (31 results) Remarks (10 results)

  • [Journal Article] Properties and cDNA cloning of the stomach chitinase from common mackerel Scomber japonicas2011

    • Author(s)
      Masahiro Matsumiya, Mana Ikeda, Keiko Hutami, Sonomi Tanaka, Miyuki Arimoto, Shinya Ito, and Kouji Miyauchi
    • Journal Title

      Advances in Chitin Science

      Volume: Volume XIII Pages: 338-343

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] 「第9回アジア太平洋キチン・キトサンシンポジウム」に参加して2011

    • Author(s)
      松宮政弘
    • Journal Title

      キチン・キトサン研究

      Volume: 17巻3号 Pages: 313-314

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 水産研究のフロントから「第23回キチン・キトサンシンポジウムに参加して」2010

    • Author(s)
      松宮政弘
    • Journal Title

      日本水産学会誌

      Volume: 76 Pages: 435-435

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Purification and Characterization of Chitinase from the Stomach of Silver Croaker Pennahia argentatus2009

    • Author(s)
      Mana Ikeda, Kouji Miyauchi, Atsushi Mochizuki, and Masahiro Matsumiya
    • Journal Title

      Protein Expression and Purification

      Volume: 65(2) Pages: 214-222

    • NAID

      10026999660

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure and functions of 42 kDa chitinase from the stomach of silver croaker Pennahia argentatus

    • Author(s)
      Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Journal Title

      9^<th> Asia-Pacific Chitin Chitosan Symposium

      Volume: (in press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Molecular cloning of a stomach chitinase in the threeline grunt Parapristipoma trilineatum

    • Author(s)
      Yujiro Kondo, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Journal Title

      9^<th> Asia-Pacific Chitin Chitosan Symposium

      Volume: (in press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Existence and physiological role of chitinase in the gonad of two species of sea hare, Kuroda's sea hare Aplysia kurodai and walking sea hare Aplysia Juliana

    • Author(s)
      Shuji Karasuda, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Journal Title

      9^<th> Asia-Pacific Chitin Chitosan Symposium

      Volume: (in press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Some enzymatic properties of a chitinase from the liver of golden cuttlefish Sepia esculenta

    • Author(s)
      Akiyoshi Suyama, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Journal Title

      9^<th> Asia-Pacific Chitin Chitosan Symposium

      Volume: (in press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Purification and characterization of a 56 kDa chitinase isozyme(PaChiB) from the stomach of silver croaker Pennahia argentatus

    • Author(s)
      Mana Ikeda, Kouji Miyauchi, and Masahiro Matsumiya
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: (in press)

    • NAID

      10030752947

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of a 56 kDa chitinase isozyme (PaChiB) from the stomach of silver croaker Pennahia argentatus

    • Author(s)
      Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Journal Title

      Biosci.Biotechnol.Biochem

      Volume: (in press)

    • NAID

      10030752947

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] マサバ胃キチナーゼアイソザイムのcDNAクローニング2011

    • Author(s)
      柿崎博美・池田愛・宮内浩二・松宮政弘
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      長崎大学
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] コウイカ肝臓キチナーゼの精製と性質2011

    • Author(s)
      須山晃佳・池田愛・宮内浩二・松宮政弘
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      長崎大学
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] イサキ胃2種のキチナーゼのcDNAクローニング2011

    • Author(s)
      近藤雄次郎・宮内浩二・松宮政弘
    • Organizer
      日本キチン・キトサン学会、第25回キチン・キトサンシンポジウム
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2011-08-31
    • Related Report
      2011 Final Research Report
  • [Presentation] アイナメ胃3種キチナーゼの構造と機能2011

    • Author(s)
      松宮政弘・南波麻里江・金田葵・木村優作・鈴木豪・浅見広太・池田愛・宮内浩二
    • Organizer
      日本キチン・キトサン学会、第25回キチン・キトサンシンポジウム
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2011-08-31
    • Related Report
      2011 Final Research Report
  • [Presentation] アイナメ胃3種キチナーゼの構造と機能2011

    • Author(s)
      松宮政弘・南波麻里江・金田葵・木村優作・鈴木豪・浅見広太・池田愛・宮内浩二
    • Organizer
      第25回キチン・キトサンシンポジウム
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] イサキ胃2種のキチナーゼのcDNAクローニング2011

    • Author(s)
      近藤雄次郎・宮内浩二・松宮政弘
    • Organizer
      第25回キチン・キトサンシンポジウム
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] シログチ胃キチナーゼアイソザイムの構造と酵素反応特性2011

    • Author(s)
      池田愛・宮内浩二・松宮政弘
    • Organizer
      日本キチン・キトサン学会、第25回キチン・キトサンシンポジウム
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2011-08-30
    • Related Report
      2011 Final Research Report
  • [Presentation] シログチ胃キチナーゼアイソザイムの構造と酵素反応特性2011

    • Author(s)
      池田愛・宮内浩二・松宮政弘
    • Organizer
      第25回キチン・キトサンシンポジウム
    • Place of Presentation
      奈良県新公会堂
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Properties and cDNA cloning of the stomach chitinase from common mackerel Scomber japonicas2011

    • Author(s)
      Masahiro Matsumiya, Mana Ikeda, Keiko Hutami, Sonomi Tanaka, Miyuki Arimoto, Shinya Ito, and Kouji Miyauchi
    • Organizer
      10th International Conference of the European Chitin Society
    • Place of Presentation
      Sain-Petersburg, Russia
    • Year and Date
      2011-05-23
    • Related Report
      2011 Final Research Report
  • [Presentation] Properties and cDNA cloning of the stomach chitinase from common mackerel Scomber japonicas2011

    • Author(s)
      Masahiro Matsumiya, Mana Ikeda, Keiko Hutami, Sonomi Tanaka, Miyuki Arimoto, Shinya Ito, Kouji Miyauchi
    • Organizer
      10^<th> International Conference of the European Chitin society
    • Place of Presentation
      Sain-Petersburg, Russia
    • Year and Date
      2011-05-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] シログチ胃キチナーゼアイソザイムの精製と性質2011

    • Author(s)
      池田愛・宮内浩二・松宮政弘
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] イサキ胃キチナーゼのcDNAクローニング2011

    • Author(s)
      近藤雄次郎・宮内浩二・松宮政弘
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Some enzymatic properties of a chitinase from the liver of golden cuttlefish Sepia esculenta2011

    • Author(s)
      Akiyoshi Suyama, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Organizer
      9^<th> Asia-Pacific Chitin Chitosan Symposium
    • Place of Presentation
      Nha Trang University(Nha Trang, Vetnam)
    • Related Report
      2011 Final Research Report
  • [Presentation] Existence and physiological role of chitinase in the gonad of two species of sea hare, Kuroda's sea hare Aplysia kurodai and walking sea hare Aplysia Juliana2011

    • Author(s)
      Shuji Karasuda, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Organizer
      9^<th> Asia-Pacific Chitin Chitosan Symposium
    • Place of Presentation
      Nha Trang University(Nha Trang, Vetnam)
    • Related Report
      2011 Final Research Report
  • [Presentation] Molecular cloning of a stomach chitinase in the threeline grunt Parapristipoma trilineatum2011

    • Author(s)
      Yujiro Kondo, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Organizer
      9^<th> Asia-Pacific Chitin Chitosan Symposium, 9^<th> Asia-Pacific Chitin Chitosan Symposium
    • Place of Presentation
      Nha Trang University(Nha Trang, Vetnam)
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure and functions of 42 kDa chitinase from the stomach of silver croaker Pennahia argentatus2011

    • Author(s)
      Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Organizer
      9^<th> Asia-Pacific Chitin Chitosan Symposium
    • Place of Presentation
      Nha Trang University(Nha Trang, Vetnam)
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure and functions of 42 kDa chitinase from the stomach of silver croaker Pennahia argentatus2011

    • Author(s)
      Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Organizer
      9^<th> Asia-Pacific Chitin Chitosan Symposium
    • Place of Presentation
      Nha Trang, Vetnam
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular cloning of a stomach chitinase in the threeline grunt Parapristipoma trilineatum2011

    • Author(s)
      Yujiro Kondo, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Organizer
      9^<th> Asia-Pacific Chitin Chitosan Symposium
    • Place of Presentation
      Nha Trang, Vetnam
    • Related Report
      2011 Annual Research Report
  • [Presentation] Existence and physiological role of chitinase in the gonad of two species of sea hare, Kuroda's sea hare Aplysia kurodai and walking sea hare Aplysia Juliana2011

    • Author(s)
      Shuji Karasuda, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Organizer
      9^<th> Asia-Pacific Chitin Chitosan Symposium
    • Place of Presentation
      Nha Trang, Vetnam
    • Related Report
      2011 Annual Research Report
  • [Presentation] Some enzymatic properties of a chitinase from the liver of golden cuttlefish Sepia esculenta2011

    • Author(s)
      Akiyoshi Suyama, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
    • Organizer
      9^<th> Asia-Pacific Chitin Chitosan Symposium
    • Place of Presentation
      Nha Trang, Vetnam
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヤマトシジミリゾチームの一次構造解析2010

    • Author(s)
      宮内浩二・松宮政弘
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      京都大学農学部
    • Year and Date
      2010-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] アイナメ胃キチナーゼアイソザイムの一次構造と酵素反応特性2010

    • Author(s)
      松宮政弘・鈴木豪・浅見広太・池田愛・宮内浩二
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      京都大学農学部
    • Year and Date
      2010-09-23
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] シログチ胃キチナーゼのcDNAクローニング2010

    • Author(s)
      池田愛・宮内浩二・松宮政弘
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      京都大学農学部
    • Year and Date
      2010-09-23
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] シログチ胃キチナーゼの精製と性質2010

    • Author(s)
      池田愛・宮内浩二・望月篤・松宮政弘
    • Organizer
      日本キチン・キトサン学会、第24回キチン・キトサンシンポジウム
    • Place of Presentation
      東京大学農学部
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] マサバ胃およびシログチ胃キチナーゼの酵素反応特性2009

    • Author(s)
      松宮政弘、伊藤伸也、池田愛、宮内浩二
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      岩手県民情報交流センター・アリーナ
    • Year and Date
      2009-10-01
    • Related Report
      2011 Final Research Report
  • [Presentation] マサバ胃およびシログチ胃キチナーゼの酵素反応特性2009

    • Author(s)
      松宮政弘, 伊藤伸也, 池田愛, 宮内浩二
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      岩手県民情報交流センター・アイーナ
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] マサバ胃キチナーゼのcDNAクローニング2009

    • Author(s)
      松宮政弘、上田光宏、宮内浩二、田中苑美、有本美雪
    • Organizer
      日本キチン・キトサン学会、第23回キチン・キトサンシンポジウム
    • Place of Presentation
      佐賀県佐賀市、佐賀大学本庄キャンパス
    • Year and Date
      2009-08-20
    • Related Report
      2011 Final Research Report
  • [Presentation] マサバ胃キチナーゼのcDNAクローニング2009

    • Author(s)
      松宮政弘, 上田光宏, 宮内浩二, 田中茄美, 有本美雪
    • Organizer
      日本キチン・キトサン学会, 第23回キチン・キトサンシンポジウム
    • Place of Presentation
      佐賀大学農学部
    • Year and Date
      2009-08-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Properties of endo-type and exo-type chitinolytic enzymes from the liver of Japanese common squid Todarodes pacificus and utilization of those for enzymatic production of N-acetyl-D-glucosamine2009

    • Author(s)
      Masahiro Matsumiya
    • Organizer
      International Symposium on Seafood Processing Technology and Safety Control System
    • Place of Presentation
      Ocean University of China, Qingdao, China
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Some properties of the stomach chitinases from two species of fish, silver croaker Pennahia argentatus and common mackerel Scomber japonicas2009

    • Author(s)
      Masahiro Matsumiya, Mana Ikeda, Ito Shinya, Kouji Miyauchi
    • Organizer
      11^<th> International Conference on Chitin and Chitosan and 8^<th> Asia-Pacific Chitin and Chitosan Symposium
    • Place of Presentation
      National Taiwan University of Science and Te chnology(NTUST), Taipei
    • Related Report
      2011 Final Research Report
  • [Presentation] Some properties of the stomach chitinases from two species of fish, silver croaker Pennahia argentatus and common mackerel Scomber japonicas2009

    • Author(s)
      松宮政弘, 池田愛, 伊藤伸也, 宮内浩二
    • Organizer
      11^<th> International Conference on Chitin and Chitosan and 8^<th> Asia-Pacific Chitin and Chitosan Symposium
    • Place of Presentation
      National Taiwan University of Science and Technology, Taipei
    • Related Report
      2009 Annual Research Report
  • [Remarks] 遺伝子番号取得(1) DDBJ number : AB686658遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (2) DDBJ number : AB686659遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (3) DDBJ number : AB626093遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (4) DDBJ number : AB626094遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (5) DDBJ number : AB605774遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (6) DDBJ number : AB605775遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (7) DDBJ number : AB689022遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (8) DDBJ number : AB642678遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (9) DDBJ number : AB642677遺伝子番号取得

    • Related Report
      2011 Final Research Report
  • [Remarks] (10) DDBJ number : AB686657遺伝子番号取得

    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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