Structure and function of crystalline chitin hydrolyzing chitinase from marine organisms

• Project/Area Number: 21580254
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Nihon University
• Principal Investigator: MATSUMIYA Masahiro 日本大学, 生物資源科学部, 教授 (60150702)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 代謝 / 酵素 / キチナーゼ / バイオマス / α-キチン / キチンオリゴ糖 / 基質分解特性 / N-アセチルグルコサミン / 遺伝子 / クローニング

Research Abstract

Crystalline chitin is a major structural structure component of arthropods. We purified several chitinases from marine organisms which take the arthropods as food and investigated the enzymatic properties and reaction. We also obtained several cDNA clones of the chitinases from marine organisms and investigated the structure and function of the chitinases.

Research Products

• [Journal Article] Properties and cDNA cloning of the stomach chitinase from common mackerel Scomber japonicas (2011)
  • Author(s): Masahiro Matsumiya, Mana Ikeda, Keiko Hutami, Sonomi Tanaka, Miyuki Arimoto, Shinya Ito, and Kouji Miyauchi
  • Journal Title: Advances in Chitin Science Volume: Volume XIII Pages: 338-343
• [Journal Article] 「第9回アジア太平洋キチン・キトサンシンポジウム」に参加して (2011)
  • Author(s): 松宮政弘
  • Journal Title: キチン・キトサン研究 Volume: 17巻3号 Pages: 313-314
• [Journal Article] 水産研究のフロントから「第23回キチン・キトサンシンポジウムに参加して」 (2010)
  • Author(s): 松宮政弘
  • Journal Title: 日本水産学会誌 Volume: 76 Pages: 435-435
• [Journal Article] Purification and Characterization of Chitinase from the Stomach of Silver Croaker Pennahia argentatus (2009)
  • Author(s): Mana Ikeda, Kouji Miyauchi, Atsushi Mochizuki, and Masahiro Matsumiya
  • Journal Title: Protein Expression and Purification Volume: 65(2) Pages: 214-222
  • NAID: 10026999660
  • Peer Reviewed
• [Journal Article] Structure and functions of 42 kDa chitinase from the stomach of silver croaker Pennahia argentatus
  • Author(s): Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Journal Title: 9^<th> Asia-Pacific Chitin Chitosan Symposium Volume: (in press)
• [Journal Article] Molecular cloning of a stomach chitinase in the threeline grunt Parapristipoma trilineatum
  • Author(s): Yujiro Kondo, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Journal Title: 9^<th> Asia-Pacific Chitin Chitosan Symposium Volume: (in press)
• [Journal Article] Existence and physiological role of chitinase in the gonad of two species of sea hare, Kuroda's sea hare Aplysia kurodai and walking sea hare Aplysia Juliana
  • Author(s): Shuji Karasuda, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Journal Title: 9^<th> Asia-Pacific Chitin Chitosan Symposium Volume: (in press)
• [Journal Article] Some enzymatic properties of a chitinase from the liver of golden cuttlefish Sepia esculenta
  • Author(s): Akiyoshi Suyama, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Journal Title: 9^<th> Asia-Pacific Chitin Chitosan Symposium Volume: (in press)
• [Journal Article] Purification and characterization of a 56 kDa chitinase isozyme(PaChiB) from the stomach of silver croaker Pennahia argentatus
  • Author(s): Mana Ikeda, Kouji Miyauchi, and Masahiro Matsumiya
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: (in press)
  • NAID: 10030752947
  • Peer Reviewed
• [Journal Article] Purification and characterization of a 56 kDa chitinase isozyme (PaChiB) from the stomach of silver croaker Pennahia argentatus
  • Author(s): Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Journal Title: Biosci.Biotechnol.Biochem Volume: (in press)
  • NAID: 10030752947
  • Peer Reviewed
• [Presentation] マサバ胃キチナーゼアイソザイムのcDNAクローニング (2011)
  • Author(s): 柿崎博美・池田愛・宮内浩二・松宮政弘
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-09-29
• [Presentation] コウイカ肝臓キチナーゼの精製と性質 (2011)
  • Author(s): 須山晃佳・池田愛・宮内浩二・松宮政弘
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2011-09-29
• [Presentation] イサキ胃2種のキチナーゼのcDNAクローニング (2011)
  • Author(s): 近藤雄次郎・宮内浩二・松宮政弘
  • Organizer: 日本キチン・キトサン学会、第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2011-08-31
• [Presentation] アイナメ胃3種キチナーゼの構造と機能 (2011)
  • Author(s): 松宮政弘・南波麻里江・金田葵・木村優作・鈴木豪・浅見広太・池田愛・宮内浩二
  • Organizer: 日本キチン・キトサン学会、第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2011-08-31
• [Presentation] アイナメ胃3種キチナーゼの構造と機能 (2011)
  • Author(s): 松宮政弘・南波麻里江・金田葵・木村優作・鈴木豪・浅見広太・池田愛・宮内浩二
  • Organizer: 第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2011-08-31
• [Presentation] イサキ胃2種のキチナーゼのcDNAクローニング (2011)
  • Author(s): 近藤雄次郎・宮内浩二・松宮政弘
  • Organizer: 第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2011-08-31
• [Presentation] シログチ胃キチナーゼアイソザイムの構造と酵素反応特性 (2011)
  • Author(s): 池田愛・宮内浩二・松宮政弘
  • Organizer: 日本キチン・キトサン学会、第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2011-08-30
• [Presentation] シログチ胃キチナーゼアイソザイムの構造と酵素反応特性 (2011)
  • Author(s): 池田愛・宮内浩二・松宮政弘
  • Organizer: 第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2011-08-30
• [Presentation] Properties and cDNA cloning of the stomach chitinase from common mackerel Scomber japonicas (2011)
  • Author(s): Masahiro Matsumiya, Mana Ikeda, Keiko Hutami, Sonomi Tanaka, Miyuki Arimoto, Shinya Ito, and Kouji Miyauchi
  • Organizer: 10th International Conference of the European Chitin Society
  • Place of Presentation: Sain-Petersburg, Russia
  • Year and Date: 2011-05-23
• [Presentation] Properties and cDNA cloning of the stomach chitinase from common mackerel Scomber japonicas (2011)
  • Author(s): Masahiro Matsumiya, Mana Ikeda, Keiko Hutami, Sonomi Tanaka, Miyuki Arimoto, Shinya Ito, Kouji Miyauchi
  • Organizer: 10^<th> International Conference of the European Chitin society
  • Place of Presentation: Sain-Petersburg, Russia
  • Year and Date: 2011-05-23
• [Presentation] シログチ胃キチナーゼアイソザイムの精製と性質 (2011)
  • Author(s): 池田愛・宮内浩二・松宮政弘
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2011-03-28
• [Presentation] イサキ胃キチナーゼのcDNAクローニング (2011)
  • Author(s): 近藤雄次郎・宮内浩二・松宮政弘
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2011-03-28
• [Presentation] Some enzymatic properties of a chitinase from the liver of golden cuttlefish Sepia esculenta (2011)
  • Author(s): Akiyoshi Suyama, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Organizer: 9^<th> Asia-Pacific Chitin Chitosan Symposium
  • Place of Presentation: Nha Trang University(Nha Trang, Vetnam)
• [Presentation] Existence and physiological role of chitinase in the gonad of two species of sea hare, Kuroda's sea hare Aplysia kurodai and walking sea hare Aplysia Juliana (2011)
  • Author(s): Shuji Karasuda, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Organizer: 9^<th> Asia-Pacific Chitin Chitosan Symposium
  • Place of Presentation: Nha Trang University(Nha Trang, Vetnam)
• [Presentation] Molecular cloning of a stomach chitinase in the threeline grunt Parapristipoma trilineatum (2011)
  • Author(s): Yujiro Kondo, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Organizer: 9^<th> Asia-Pacific Chitin Chitosan Symposium, 9^<th> Asia-Pacific Chitin Chitosan Symposium
  • Place of Presentation: Nha Trang University(Nha Trang, Vetnam)
• [Presentation] Structure and functions of 42 kDa chitinase from the stomach of silver croaker Pennahia argentatus (2011)
  • Author(s): Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Organizer: 9^<th> Asia-Pacific Chitin Chitosan Symposium
  • Place of Presentation: Nha Trang University(Nha Trang, Vetnam)
• [Presentation] Structure and functions of 42 kDa chitinase from the stomach of silver croaker Pennahia argentatus (2011)
  • Author(s): Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Organizer: 9^<th> Asia-Pacific Chitin Chitosan Symposium
  • Place of Presentation: Nha Trang, Vetnam
• [Presentation] Molecular cloning of a stomach chitinase in the threeline grunt Parapristipoma trilineatum (2011)
  • Author(s): Yujiro Kondo, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Organizer: 9^<th> Asia-Pacific Chitin Chitosan Symposium
  • Place of Presentation: Nha Trang, Vetnam
• [Presentation] Existence and physiological role of chitinase in the gonad of two species of sea hare, Kuroda's sea hare Aplysia kurodai and walking sea hare Aplysia Juliana (2011)
  • Author(s): Shuji Karasuda, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Organizer: 9^<th> Asia-Pacific Chitin Chitosan Symposium
  • Place of Presentation: Nha Trang, Vetnam
• [Presentation] Some enzymatic properties of a chitinase from the liver of golden cuttlefish Sepia esculenta (2011)
  • Author(s): Akiyoshi Suyama, Mana Ikeda, Kouji Miyauchi, Masahiro Matsumiya
  • Organizer: 9^<th> Asia-Pacific Chitin Chitosan Symposium
  • Place of Presentation: Nha Trang, Vetnam
• [Presentation] ヤマトシジミリゾチームの一次構造解析 (2010)
  • Author(s): 宮内浩二・松宮政弘
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都大学農学部
  • Year and Date: 2010-09-24
• [Presentation] アイナメ胃キチナーゼアイソザイムの一次構造と酵素反応特性 (2010)
  • Author(s): 松宮政弘・鈴木豪・浅見広太・池田愛・宮内浩二
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都大学農学部
  • Year and Date: 2010-09-23
• [Presentation] シログチ胃キチナーゼのcDNAクローニング (2010)
  • Author(s): 池田愛・宮内浩二・松宮政弘
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都大学農学部
  • Year and Date: 2010-09-23
• [Presentation] シログチ胃キチナーゼの精製と性質 (2010)
  • Author(s): 池田愛・宮内浩二・望月篤・松宮政弘
  • Organizer: 日本キチン・キトサン学会、第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京大学農学部
• [Presentation] マサバ胃およびシログチ胃キチナーゼの酵素反応特性 (2009)
  • Author(s): 松宮政弘、伊藤伸也、池田愛、宮内浩二
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 岩手県民情報交流センター・アリーナ
  • Year and Date: 2009-10-01
• [Presentation] マサバ胃およびシログチ胃キチナーゼの酵素反応特性 (2009)
  • Author(s): 松宮政弘, 伊藤伸也, 池田愛, 宮内浩二
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 岩手県民情報交流センター・アイーナ
  • Year and Date: 2009-10-01
• [Presentation] マサバ胃キチナーゼのcDNAクローニング (2009)
  • Author(s): 松宮政弘、上田光宏、宮内浩二、田中苑美、有本美雪
  • Organizer: 日本キチン・キトサン学会、第23回キチン・キトサンシンポジウム
  • Place of Presentation: 佐賀県佐賀市、佐賀大学本庄キャンパス
  • Year and Date: 2009-08-20
• [Presentation] マサバ胃キチナーゼのcDNAクローニング (2009)
  • Author(s): 松宮政弘, 上田光宏, 宮内浩二, 田中茄美, 有本美雪
  • Organizer: 日本キチン・キトサン学会, 第23回キチン・キトサンシンポジウム
  • Place of Presentation: 佐賀大学農学部
  • Year and Date: 2009-08-20
• [Presentation] Properties of endo-type and exo-type chitinolytic enzymes from the liver of Japanese common squid Todarodes pacificus and utilization of those for enzymatic production of N-acetyl-D-glucosamine (2009)
  • Author(s): Masahiro Matsumiya
  • Organizer: International Symposium on Seafood Processing Technology and Safety Control System
  • Place of Presentation: Ocean University of China, Qingdao, China
• [Presentation] Some properties of the stomach chitinases from two species of fish, silver croaker Pennahia argentatus and common mackerel Scomber japonicas (2009)
  • Author(s): Masahiro Matsumiya, Mana Ikeda, Ito Shinya, Kouji Miyauchi
  • Organizer: 11^<th> International Conference on Chitin and Chitosan and 8^<th> Asia-Pacific Chitin and Chitosan Symposium
  • Place of Presentation: National Taiwan University of Science and Te chnology(NTUST), Taipei
• [Presentation] Some properties of the stomach chitinases from two species of fish, silver croaker Pennahia argentatus and common mackerel Scomber japonicas (2009)
  • Author(s): 松宮政弘, 池田愛, 伊藤伸也, 宮内浩二
  • Organizer: 11^<th> International Conference on Chitin and Chitosan and 8^<th> Asia-Pacific Chitin and Chitosan Symposium
  • Place of Presentation: National Taiwan University of Science and Technology, Taipei
• [Remarks] 遺伝子番号取得(1) DDBJ number : AB686658遺伝子番号取得
• [Remarks] (2) DDBJ number : AB686659遺伝子番号取得
• [Remarks] (3) DDBJ number : AB626093遺伝子番号取得
• [Remarks] (4) DDBJ number : AB626094遺伝子番号取得
• [Remarks] (5) DDBJ number : AB605774遺伝子番号取得
• [Remarks] (6) DDBJ number : AB605775遺伝子番号取得
• [Remarks] (7) DDBJ number : AB689022遺伝子番号取得
• [Remarks] (8) DDBJ number : AB642678遺伝子番号取得
• [Remarks] (9) DDBJ number : AB642677遺伝子番号取得
• [Remarks] (10) DDBJ number : AB686657遺伝子番号取得