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Characterization of inhibitory factors of lactic acid bacteria against gastrointestinal pathogens

Research Project

Project/Area Number 21580334
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionKitasato University

Principal Investigator

MUKAI Takao  北里大学, 獣医学部, 教授 (20229917)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords乳酸菌 / プロバイオティクス / 付着 / フィブロネクチン / 表層タンパク質 / 感染予防 / キャンピロバクター / 感染 / Helicobacter pylori
Research Abstract

The goal of our study was to elucidate the capacity of the Lactobacillus reuteri and Lactobacillus gasseri to competitively exclude gastrointestinal pathogens. On the basis of the previous results, this study was firstly conducted to determine the adhesion factor of L. reuteri JCM1081 to the sulfated sugar chains of sulfatide, which was recognized as a receptor moiety of Helicobacter pylori. The potential adhesion factor, Ef-Tu was expressed as a recombinant protein followed by kinetics studies. Ef-Tu of the strain was shown to be a high affinity for sulfatide and to be retained tightly on the cell surface at pH 4.0 but not to pH6.0.The findings strongly suggested that Ef-Tu is a novel adhesion factor the sulfated sugar chains of sulfatide to in Lactobacillus strains.
Secondly, the capacity of fibronectin-adherent L. gasseri TM333 selected previously to competitively exclude Campylobacter jejuni 81-176 in chicks was examined. Chicks were given daily oral doses of L. gasseri TM333 for 7 days after oral infection with C. jejuni 81-176.The chicks treated with L. gasseri TM333 showed 98% inhibition of cecum colonization by C. jejuni 81-176.The findings indicate the fibronectin-adherent L. gasseri TM333 effectively inhibited Campylobacter colonization of the chick cecum.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (18 results)

All 2012 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (13 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Photodynamic antimicrobial activity of avian eggshell pigments2010

    • Author(s)
      Ishikawa SI, et al.
    • Journal Title

      FEBS Letters 584

      Pages: 770-774

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 陽内細菌とプロバイオティクス2010

    • Author(s)
      向井孝夫
    • Journal Title

      東北のコロニー 34

      Pages: 13-20

    • Related Report
      2009 Annual Research Report
  • [Presentation] Lactobacillus reuteri JCM1081のEF-Tuの硫酸化糖鎖に対する結合特性の評価2012

    • Author(s)
      西山啓太、坪川大悟、中光貴之、山本裕司、市川尊文、五艘行信、石原和彦、向井孝夫
    • Organizer
      日本畜産学会,第115回大会講演要旨
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Lactobacillus reuteri JCM1081のEF-Tuの硫酸化糖鎖に対する結合特性の評価2012

    • Author(s)
      西山啓太, ら
    • Organizer
      日本畜産学会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Lactobacillus rhamnosusのInternalin Jのラミニンへの付着特性の解析2012

    • Author(s)
      仲又幸一, ら
    • Organizer
      日本畜産学会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] L. gasseri SBT2055のAggregation-promorting factorのフィブロネクチンの付着特性2011

    • Author(s)
      西山啓太、中里彩季子、山本裕司、瀬戸泰行、中島肇、向井孝夫
    • Organizer
      日本酪農科学学会60周年記念大会
    • Place of Presentation
      フォレスト仙台
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] L.gasseri SBT2055のAggregation-promorting factorのフィブロネクチンの付着特性2011

    • Author(s)
      西山啓太, ら
    • Organizer
      日本酪農科学会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Campylobacterと同一のレセプターに結合するLactobacillus gasseriによるin vivo感染阻害2011

    • Author(s)
      西山啓太、角田勤、高井伸二、山本裕司、瀬戸泰幸、中島肇、向井孝夫
    • Organizer
      日本畜産学会,第114回大会講演要旨
    • Place of Presentation
      北里大学
    • Year and Date
      2011-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] ampylobacterと同一のレセプターに結合するLactobacillus gasseriによるin vivo感染阻害2011

    • Author(s)
      西山啓太, ら
    • Organizer
      日本畜産学会
    • Place of Presentation
      北里大学(十和田市)
    • Year and Date
      2011-08-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] ビフィズス菌のムチンへの付着方法の確立2011

    • Author(s)
      清宮広貴, ら
    • Organizer
      日本畜産学会
    • Place of Presentation
      北里大学(十和田市)
    • Year and Date
      2011-08-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 純度の異なるブタムチンへのビフィズス菌の付着性2011

    • Author(s)
      西山啓太, ら
    • Organizer
      日本乳酸菌学会
    • Place of Presentation
      関西大学(大阪市)
    • Year and Date
      2011-07-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Lactobacillus reuteri JCM1081のElongation Factor-Tuの胃腸粘膜への付着因子としての評価2010

    • Author(s)
      西山啓太、浜野太一、金子仁美、山本裕司、向井孝夫
    • Organizer
      日本乳酸菌学会2010年度大会
    • Place of Presentation
      フォレスト仙台
    • Year and Date
      2010-07-26
    • Related Report
      2011 Final Research Report
  • [Presentation] L.reuteri JCM1081のElongation Factor-Tuの胃腸粘膜への付着因子としての評価2010

    • Author(s)
      西山啓太, 向井孝夫, 他2名
    • Organizer
      日本乳酸菌学会
    • Place of Presentation
      フォレスト仙台(仙台)
    • Year and Date
      2010-07-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] L. reuteriの推定付着因子であるEF-TuのpH依存的な付着特性の変化2010

    • Author(s)
      西山啓太、浜野太一、金子仁美、山本裕司、向井孝夫
    • Organizer
      日本畜産学会,第112回大会講演要旨
    • Place of Presentation
      明治大学
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] L.reuteriJCM1081の推定付着因子であるEF-TuのpH依存的な付着特性の変化2010

    • Author(s)
      西山啓太、向井孝夫, ほか3名
    • Organizer
      日本畜産学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Book] Lactobacilusの腸粘膜への付着に関与する菌体表層糖質乳酸菌とビフィズス菌のサイエンス2010

    • Author(s)
      向井孝夫
    • Publisher
      京都大学学術出版会
    • Related Report
      2011 Final Research Report
  • [Book] 食の安全と予防医学(分担執筆)2009

    • Author(s)
      向井孝夫
    • Total Pages
      29
    • Publisher
      養賢堂
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://d.hatena.ne.jp/bunshi/

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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