| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
To elucidate the causes of low fertility in modern high producing dairy cows, we investigated the characteristics of oocyte maturation and fertilization. As the results, the time of ovulation after LH surge and the ultrastructure of in vivo matured preovulatory oocytes were revealed. The time required for the sperm transport to the oviduct reservoir and functional life span of ovulated oocytes were estimated. By using in vitro mature oocytes, we defined the characters of acquisition of developmental capacity. Moreover, we developed a novel treatment of repeat breeding cows by using seminal plasma proteins, and a new in-vitro oocyte growth culture system to examine the effects of stresses on oocyte growth and maturation. The possibility of prediction of the time of ovulation and abnormal ovulation by monitoring vaginal temperature changes was also indicated
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