Investigation of oocyte maturation and fertilizati in high-producing and sub-fertile dairy cows
| Project/Area Number |
21580339
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KATAGIRI Seiji 酪農学園大学, 獣医学部, 教授 (00292061)
NAGANAO Masashi 北海道大学, 大学院・獣医学研究科, 准教授 (70312402)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 牛 / 繁殖 / 受胎 / 卵子成熟 / 受精 / 卵子発生 / 高泌乳牛 / 低受胎牛 / 卵子発生能 |
|---|
| Research Abstract |
To elucidate the causes of low fertility in modern high producing dairy cows, we investigated the characteristics of oocyte maturation and fertilization. As the results, the time of ovulation after LH surge and the ultrastructure of in vivo matured preovulatory oocytes were revealed. The time required for the sperm transport to the oviduct reservoir and functional life span of ovulated oocytes were estimated. By using in vitro mature oocytes, we defined the characters of acquisition of developmental capacity. Moreover, we developed a novel treatment of repeat breeding cows by using seminal plasma proteins, and a new in-vitro oocyte growth culture system to examine the effects of stresses on oocyte growth and maturation. The possibility of prediction of the time of ovulation and abnormal ovulation by monitoring vaginal temperature changes was also indicated
|
Report
(4 results)
Research Products
(15 results)
