| Project/Area Number |
21580344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Yamaguchi University (2011)
The University of Tokyo (2009-2010) |
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Principal Investigator |
KANO Kiyoshi 山口大学, 農学部, 准教授 (40312516)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMATSU Masami 北海道大学, 遺伝子病制御研究所, 准教授 (70241370)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 発生工学 / ボディサイズ / マウス / トランスジェニックマウス / 器官のサイズ / DDR2 / 個体の成長 |
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| Research Abstract |
We analyzed phenotypes of DDR2 transgenic mice and ATDC5 cell lines with a focus on growth and size control. In previous study, cartilage cell proliferation decreased in DDR2 KO mice, and we analyzed also the function of DDR2 in cartilage tissue. We produced Ddr2 cDNA transgenic mice, which were specifically overexpressed only in the cartilage cells, but there were no prominent phenotypes in cartilage tissue of the transgenic mice.
To understand the role of Ddr2 in chondrocyte proliferation and differentiation, we next performed knockdown experiments using Ddr2 miRNA in ATDC5 cells. We found that DDR2 might repress cell proliferation and differentiation in cartilage cells.
DDR2 is suggested to have important function especially in the reproductive organs by the expression in various tissues.
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