| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
We showed that rs41648172、rs41648176 and rs41648178 SNPs have differences in allele frequency distribution between high-marbled and low-marbled cattle groups of Japanese Black breed, by performing detection of PNLIP polymorphism with direct-sequencing and establishment of genotyping method with PCR-RFLP. We further clarified that rs41648172 SNP is useful marker for marker-assisted selection to increase marbling level, using association study for individual SNP effect, analysis of comparison of allele frequency distribution between various cattle breeds, and association study for combined marker effect.
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