Development of a general method for obtaining double KO swine cells which will be used as donors for production of cloned homozygous piglets
Project/Area Number |
21580350
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied animal science
|
Research Institution | Kagoshima University |
Principal Investigator |
SATO Masahiro 鹿児島大学, フロンティアサイエンス研究推進センター, 教授 (30287099)
|
Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Mitsutoshi 鹿児島大学, 農学部, 教授 (00174954)
MIYOSHI Kazuchika 鹿児島大学, 農学部, 准教授 (70363611)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | KOブタ / ホモ / gene targeting / 薬剤耐性 / 体細胞核移植 / 胎仔性繊維芽細胞 / 遺伝子導入 / double KO / ホモKOブタ / gene targetin / ホセKOブタ |
Research Abstract |
This study aims to develop a general method for obtaining double KO swine cels in which the target gene expression is completely blocked. This was successfully performed by selection of heterogenous KO cels under higher concentrations of selective drugs after repeated delivery of a targeting vector(RDTV). These resulting double KO cells will be used for somatic cell nuclear transfer-mediated production of cloned pigs.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] Innate immune system still works at diapause, a physiological state of dormancy in insects2011
Author(s)
Nakamura A, Takezawa Y, Ohnami N, Sato M, Ono C, Harada Y, Yoshida K, Kawano N, Kanai S, Miyado M, Umezawa A
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Journal Title
Biochemical and Biophysical Research Communication
Volume: 410
Pages: 351-357
Related Report
Peer Reviewed
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[Journal Article] Beta-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion2011
Author(s)
Takezawa Y, Yoshida K, Miyado K, Sato M, Nakamura A, Kawano N, Sakakibara K, Kondo T, Harada Y, Ohnami N, Kanai S, Miyado M, Saito H, Takahashi Y, Akutsu H, Umezawa A
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Journal Title
Scientific Reports
Volume: 1
Pages: 68-68
Related Report
Peer Reviewed
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