Why do not obese bears suffer fatty liver?-Study on mechanism for accumulation of body fat during pre-hibernation period

• Project/Area Number: 21580355
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: TSUBOTA Toshio 北海道大学, 大学院・獣医学研究科, 教授 (10207441)
• Co-Investigator(Kenkyū-buntansha): SHIMOZU Rumichito 北海道大学, 大学院・獣医学研究科, 助教 (50507168)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: クマ / 冬眠 / 肥満 / 体脂肪 / グルコース / 静注糖負荷試験 / インスリン / 脂肪肝 / 中性脂肪 / コレステロール / 遊離脂肪酸 / 静注負荷試験

Research Abstract

This study was conducted in Ani Mataginosato Bear Park, Kitaakita-shi, Akita Prefecture in order to know why obese bears do not suffer fatty liver even though they get 30-40% fat of body weight and to investigate a feature mechanism for accumulation of body fat. Under anesthetized condition, measurements of blood concentrations of carbohydrate and fat, intravenous glucose tolerance tests and adipose tissue biopsies were performed for this study. As results, it is concluded that increase of body fat accumulation during pre-hibernation period is controlled by physiological and metabolic mechanism as well as hyperphagia in Japanese black bears

Research Products

• [Journal Article] Changes in blood glucose and insulin responses to intravenous glucose tolerance tests and blood biochemical values in adult female Japanese black bears (Ursus thibetanus japonicus) (2012)
  • Author(s): Kamine, A., Shimozuru, M., Shibata, H. Tsubota, T
  • Journal Title: n. J. Vet. Res Volume: 60(1) Pages: 5-13
  • NAID: 120003860277
• [Journal Article] Effects of tiletamine-zolazepam with and without premedications on blood chemistry and glucose tolerance tests in Japanese black bears (Ursus thibetanus japonicus) (2012)
  • Author(s): ○Kamine A, Shimozuru M, et al.
  • Journal Title: American Journal of Veterinary Research Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Changes in blood glucose and insulin responses to intravenous glucose tolerance tests and blood biochemical values in adult female Japanese black bears (Ursus thibetanus japonicus) (2012)
  • Author(s): ○Kamine A, Shimozuru M, et al.
  • Journal Title: Japanese Journal of Veterinary Research Volume: 60 Pages: 5-13
  • NAID: 120003860277
  • Peer Reviewed
• [Journal Article] ニホンツキノワグマにおける冬眠前の脂肪蓄積メカニズム.獣医畜産新報 (2010)
  • Author(s): 加味根あかり・下鶴倫人・坪田敏男
  • Journal Title: 獣医畜産新報 Volume: 63(5) Pages: 367-370
• [Journal Article] ニホンツキノワグマにおける繁殖と母体栄養状態の関連性 (2010)
  • Author(s): ○中村幸子、岡野司, 他
  • Journal Title: 獣医畜産新報 Volume: 63 Pages: 361-365
• [Journal Article] ニホンツキノワグマにおける冬眠前の脂肪蓄積メカニズム (2010)
  • Author(s): ○加味根あかり、下鶴倫人、坪田敏男
  • Journal Title: 獣医畜産新報 Volume: 163 Pages: 367-370
• [Journal Article] Change in body weight of mothers and neonates and in milk composition during denning period in captive Japanese black bears (Ursus thibetanus japonicus) (2009)
  • Author(s): Iibuchi, R., Nakano, N., Nakamura, T., Urashima, T., Shimozuru, M., Murase, T. & Tsubota, T
  • Journal Title: Jpn. J. Vet. Res Volume: 57 Pages: 13-22
  • NAID: 120001400760
• [Journal Article] Change in body weight of mothers and neonates and in milk composition during denning period in captive Japanese black bears (Ursus thibetanus japonicus) (2009)
  • Author(s): Iibuchi, R., Nakano, N., et al.
  • Journal Title: Japanese Journal of Veterinary Research 57 Pages: 13-22
  • Peer Reviewed
• [Journal Article] Effects of tiletamine-zolazepam with and without premedications on blood chemistry and glucose tolerance tests in Japanese black bears (Ursus thibetanus japonicus)
  • Author(s): Kamine, A., Shimozuru, M., Shibata, H. Tsubota, T
  • Journal Title: Am. J. Vet. Res Volume: (in press)
• [Presentation] Changes in expression of hepatic genes involved in energy metabolism during the hibernation period in Japanese black bears (2011)
  • Author(s): Shimozuru, M., Kamine, A., & Tsubota, T
  • Organizer: 20th International Conference on Bear Research & Management
  • Place of Presentation: Ottawa, Canada
• [Presentation] Changes in expression of hepatic genes involved in energy metabolism during the hibernation period in Japanese black bears (2011)
  • Author(s): ○Shimozuru M, Kamine A, et al
  • Organizer: 20h International Conference on Bear Research and Management
  • Place of Presentation: The Westin Ottawa (Canada)
• [Presentation] Changes in biochemical values of captive Japanese black bears during active and hibernation phases (2010)
  • Author(s): Kamine, A., Iibuchi, R., Shimozuru, M. & Tsubota, T
  • Organizer: 19th International Conference on Bear Research & Management
  • Place of Presentation: Tbilisi, Georgia
• [Presentation] Changes in biochemical values of captive Japanese black bears during active and hibernation phases (2010)
  • Author(s): ○Kamine A, Iibuchi R, et al.
  • Organizer: 19h International Conference on Bear Research and Management
  • Place of Presentation: Tbilisi, Georgia(グルジア)
• [Presentation] Physiological and endocrinological mechanism for nutritional condition and reproduction related to hibernation in the Japanese black bear (Ursus thibetanus japonicas) (2009)
  • Author(s): Tsubota, T., Nakamura, S., Iibuchi, R.,Kamine, A., Shimozuru, M
  • Organizer: International Symposium on Conservation of Asiatic Black Bear
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2009-11-21
• [Presentation] Physiological and endocrinological mechanism for nutritional condition and reproduction related to hibernation in the Japanese black bear(Ursus thibetanus japonicus) (2009)
  • Author(s): Tsubota, T., Nakamura, S., et al.
  • Organizer: 2009 International Symposium on conservation of Asiatic black bear
  • Place of Presentation: 台北市、台北動物園(台湾)
  • Year and Date: 2009-11-17
• [Presentation] ニホンツキノワグマにおける冬眠前の脂肪蓄積に関する代謝メカニズムの解明 (2009)
  • Author(s): 加味根あかり、中村幸子, 他
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 宇都宮市、栃木県総合文化センター
  • Year and Date: 2009-04-03
• [Presentation] TPR profiles, and plasma glucose and fat concentrationsof male Japanese black bears (Ursus thibetanus japonicas) during hibernation (2009)
  • Author(s): Iibuchi, R., Kamine, A., Shimozuru, M. & Tsubota, T
  • Organizer: The 3rd international Workshop of Asian Society of Zoo and Wildlife medicine & The 1' Meeting of Korean Society of Zoo and Wildlife Medicine
  • Place of Presentation: Seoul, Korea
• [Presentation] ニホンツキノワグマにおける冬眠前の脂肪蓄積に関する代謝メカニズムの解明 (2009)
  • Author(s): 加味根あかり,中村幸子,飯渕るり子,山中淳史,柴田治樹,下鶴倫人,坪田敏男
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 栃木
• [Presentation] TPR profiles and plasma glucose and fat concentrations of male Japanese black bears(Ursus thibetanus japonicus)during hibernation (2009)
  • Author(s): Iibuchi, R., Kamine, A., et al.
  • Organizer: The 3^<rd> International Workshop of Asian Society of Zoo and Wildlife Medicine
  • Place of Presentation: Seoul, Seoul National University(Korea)
• [Book] 日本のクマ-ヒグマとツキノワグマの生物学- (2011)
  • Author(s): 坪田敏男・山崎晃司編
  • Publisher: 東京大学出版会
• [Book] 日本のクマーヒグマとツキノワグマの生物学 (2011)
  • Author(s): 坪田敏男、山崎晃司編
  • Total Pages: 370
  • Publisher: 東京大学出版社
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.jp/wildlifeBilolgy01.html
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.jp/wildlifeBiology01.html
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.jp/wildlifeBiology01.html