Investigation for physiological roles of glucocorticoid in the periovulatory follicle

• Project/Area Number: 21580357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: TETSUKA Masafumi 帯広畜産大学, 畜産学部, 教授 (40311526)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 排卵 / 炎症 / 糖質コルチコイド / サイトカイン / 卵母細胞 / 11β-HSD / 糖質コルチコイド受容体 / 生理学 / 細胞・組織 / 酵素

Research Abstract

Ovulation in mammal is an inflammatory reaction and the oocyte undergoes this process is likely to be influenced by various inflammatory factors. In this study we investigated the presence and action of enzymes that catalyze anti-inflammatory glucocorticoid (G) and inflammatory cytokine IL-1 in the bovine cumulus-oocyte complex (COC). Expression and action of enzymes that activate/inactivate G as well as IL-1 and its functional receptor are demonstrated in COC. These results suggest that G and IL-1 act together to regulate functions of periovulatory bovine COC.

Research Products

• [Journal Article] Gene Expression of 11.BETA.-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia: The Role of Follicular Stimulating Hormone (2010)
  • Author(s): Tetsuka M, et al.
  • Journal Title: Journal of Reproduction and Development Volume: 56 Issue: 6 Pages: 616-622
  • DOI: 10.1262/jrd.10-019K
  • NAID: 130000305915
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Gene expression of 11beta-HSD and glucocorticoid receptor in the bovine (bos taurus) follicle during follicular maturation and at resia : the role of follicular stimulating hormone. (2010)
  • Author(s): Tetsuka M, et al.
  • Journal Title: Journal of Reproduction and Development Volume: 56 Pages: 616-622
  • Peer Reviewed
• [Presentation] IL-1ss directly acts on bovine oocyte to induce nuclear maturation (2011)
  • Author(s): Onuma A, et al
  • Organizer: 2nd World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2011-10-10
• [Presentation] 成熟培養時のウシ卵丘卵母細胞複合体におけるIL-1βの卵子核成熟促進作用 (2011)
  • Author(s): 小沼あすか, ほか
  • Organizer: 第104回日本繁殖生物学会大会
  • Place of Presentation: 岩手大学(盛岡)
  • Year and Date: 2011-09-15
• [Presentation] IL-1β directly acts on bovine oocyte to induce nuclear maturation (2010)
  • Author(s): Onuma A, Akasaka K, Tetsuka M
  • Organizer: 2nd World Congress on Reproductive Biology
  • Place of Presentation: Cairns Convention Centre, Cairns, Australia
  • Year and Date: 2010-10-10
• [Presentation] Changes in gene expression and metabolic activity of 11-HSD in bovine cumulus-oocyte complex during maturation culture (2010)
  • Author(s): Tetsuka M, Takeuchi T, Okawara S, Ota S, Onuma A, Akasaka K, Hamano S
  • Organizer: 14th International Congress on Hormonal Steroids and Hormones & Cancer
  • Place of Presentation: University of Edinburgh, Edinburgh, UK.
  • Year and Date: 2010-09-23
• [Presentation] Changes in gene expression and metabolic activity of 11β-HSD in bovine cumulus-oocyte complex during maturation culture (2010)
  • Author(s): Tetsuka M, et al.
  • Organizer: 14th International Congress on Hormonal Steroids and Hormones & Cancer
  • Place of Presentation: University of Edinburgh Edinburgh, Scotland
  • Year and Date: 2010-09-22
• [Presentation] 成熟培養時のウシ卵丘卵母細胞複合体におけるIL-1βの卵子核成熟促進作用 (2010)
  • Author(s): 小沼あすか,赤坂清美,手塚雅文
  • Organizer: 第104回日本繁殖生物学会大会
  • Place of Presentation: 岩手大学,盛岡市
  • Year and Date: 2010-09-15
• [Presentation] 成熟培養に伴うウシ卵丘卵母細胞複合体における糖質コルチコイド代謝の変化 (2010)
  • Author(s): 手塚雅文,竹内拓勝,太田慎太郎,小沼あすか,赤坂清美
  • Organizer: 第103回日本繁殖生物学会大会
  • Place of Presentation: 北里大学獣医学部,十和田市
  • Year and Date: 2010-09-04
• [Presentation] 成熟培養に伴うウシ卵丘卵母細胞複合体における糖質コルチコイド代謝の変化 (2010)
  • Author(s): 手塚雅文, ほか
  • Organizer: 第103回日本繁殖生物学会大会
  • Place of Presentation: 北里大学獣医学部十和田キャンパス
  • Year and Date: 2010-09-04
• [Remarks] 帯広畜産大学学術情報リポジトリ
  • URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2810
• [Remarks] 帯広畜産大学学術情報リポジトリ
  • URL: http://ir.obihiro.ac.jp/dspace/