Three-dimensional ultrastructural study of Endothelial cells and pericytes interdigitation in angiogenesis
Project/Area Number |
21580371
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Basic veterinary science/Basic zootechnical science
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Research Institution | Azabu University |
Principal Investigator |
WAKUI Shin 麻布大学, 獣医学部, 准教授 (40201157)
|
Project Period (FY) |
2009 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 病理 / 血管新生制御 / 血管新生 / 血管内皮細胞 / 血管周細胞 / 細胞質突起相互陥入 / 周細胞 / 相互陥入部 / 三次元電顕免疫学 |
Research Abstract |
Endothelial cells and pericytes play critical role in angiogenesis by the angiopoietin system. Endothelial cell and pericyte interdigitations (EPI), consisting of cytoplasmic projections of pericytes and corresponding endothelial indentations, are frequently present at angiogenic sites. Rats subcutaneous implantation of a thermoreversible gelation polymer discs were inqubated in vitro 24-72hr. The capillary density increased to a peak on 48hr. A number of EPI were observed to a peak on 72hr. Serial sectioning electron microscopy immunohistochemical analysis revealed that Angiopoietin-1 localized at Pericytes and EPI. Quantitative RT-PCR analysis revealed that the level of Angiopoietin-1 increased on 72hr. The present study showed that the microvesselmaturation might to be involved in the expression of EPI and Angiopoietin-1.
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Report
(5 results)
Research Products
(33 results)
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[Journal Article] Atypical Leydig cell hyperplasia in adult rats with low T and high LH induced by prenatal di(n-butyl) phthalate exposure.2013
Author(s)
Wakui, S., Takahashi, H., Muto, T., Shirai,M., Jutabha, P., Anzai,N., Wempe, M.F. Kansaku, N., Hano, H., Inomata,T. and Endou,H.
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Journal Title
Toxicol. Pathol
Volume: 41
Pages: 480-486
Related Report
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[Journal Article] Mrophometorical analysis on preneoplastic cellular nuclei in BBN induced rat bladder carcinogenesis2012
Author(s)
Wakui, S., Mtohashi, M., Imai,K.,Kobayashi, Y., Tanaka, I., Nishimoto, T., Sato, T., Muto, T., Takahashi, H. and Hano, H
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Journal Title
J. Toxicol. Pathol
Volume: 25
Related Report
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[Journal Article] Sertoli cellsproliferate in adult rats with prenatalexposure to 3,3',4,4',5-pentachlorobiphenyl2012
Author(s)
Wakui, S., Muto, T., Suzuki, Y., Takahashi, H. and Hano, H
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Journal Title
Arch. Toxicol
Volume: 86
Pages: 159-162
Related Report
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[Journal Article] Metabolism and pharmacokineticstudies of JPH203, an L-amino acidtransporter2012
Author(s)
Wempe, M.F., Rice, P.J., Lightner, J.W.,Jutabha, P., Hayashi, M., Anzai, N., Wakui, S., Kusuhara, H., Sugiyama, Y. and Endou, H
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Journal Title
Drug Metab. Pharmacokineti
Volume: 27
Pages: 155-161
Related Report
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[Journal Article] Potent hURAT1 inhibitors: in vitro andin vivo metabolism and pharmacokinetic studies2012
Author(s)
Wempe, M.F., Rice, P.J., Lightner, J.W.,Jutabha, P., Hayashi, M., Anzai, N., Wakui, S., Kusuhara, H., Sugiyama, Y. and Endou, H
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Journal Title
Drug Design, Development and Therapy
Volume: 6
Pages: 323-339
Related Report
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[Journal Article] Mrophometorical analysis on preneoplastic cellular nuclei inBBN induced rat bladder carcinogenesis.2012
Author(s)
Wakui, S., Mtohashi, M., Imai, K.,Kobayashi, Y., Tanaka, I., Nishimoto, T., Sato, T., Muto, T.,Takahashi, H. and Hano, H.
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Journal Title
J. Toxicol. Pathol.
Volume: 25
Related Report
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[Journal Article] Sertoli cells proliferate in adult rats with prenatal exposure to 3,3’,4,4’,5-pentachlorobiphenyl.2012
Author(s)
Wakui, S., Muto, T., Suzuki, Y., Takahashi, H. and Hano, H.
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Journal Title
Arch. Toxicol.
Volume: 86
Pages: 159-162
Related Report
Peer Reviewed
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[Journal Article] Metabolism and pharmacokinetic studies of JPH203, an L-amino acid transporter.2012
Author(s)
Wempe, M.F., Rice, P.J., Lightner, J.W., Jutabha, P., Hayashi, M., Anzai, N., Wakui, S., Kusuhara, H., Sugiyama, Y. and Endou, H.
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Journal Title
Drug Metab. Pharmacokineti.
Volume: 27
Pages: 155-161
Related Report
Peer Reviewed
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[Journal Article] Potent hURAT1 inhibitors: in vitro and in vivo metabolism and pharmacokinetic studies.2012
Author(s)
Wempe, M.F., Rice, P.J., Lightner, J.W., Jutabha, P., Hayashi, M., Anzai, N., Wakui, S., Kusuhara, H., Sugiyama, Y. and Endou, H.
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Journal Title
Drug Design, Development and Therapy
Volume: 6
Pages: 323-339
Related Report
Peer Reviewed
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[Journal Article] Testicularspermiation failure in rats exposed prenatally to 3,3′,4,4′,5- pentachlorobiphenyl2011
Author(s)
Wakui, S., Muto, T., Motohashi, M.,Kobayashi, Y., Suzuki, Y., Takahashi, H. and Hano
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Journal Title
J. Toxicol. Sci
Volume: 35
Pages: 757-765
Related Report
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[Journal Article] Mrophometorical analysis on preneoplastic cellular nuclei in BBN induced rat bladder carcinogenesis.2011
Author(s)
Wakui, S., Imai, K., Mtohashi, M.,Kobayashi, Y., Tanaka, I., Nishimoto, T., Sato, T., Muto, T., Takahashi, H. and Hano, H
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Journal Title
J. Toxicol. Pathol
Volume: 24
Related Report
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[Journal Article] MicroPNAchanges induced by heterocyclic amines and its significance in the early satges of colon charcinogenesis2011
Author(s)
Imai, K., Ochiai, M., Hippo, Y., Igarashi,M., Wakui, S. and Nakagama, H
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Journal Title
J. Toxicol. Pathol
Volume: 24
Related Report
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[Journal Article] Sex-associated difference in estrogen receptor βexpression in N-methyl-N′-nitro-N- nitrosoguanidine-induced rat gastric cancers2011
Author(s)
Wakui, S., Motohashi, M., Muto, T., Takahashi, H., Hano, H., Jutabha, P., Anzai,N., Wempe, M.F. and Endou, H
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Journal Title
Comparative. Med
Volume: 61
Pages: 1-7
Related Report
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[Journal Article] Cyclin D1/cdk4, estrogen receptors α and β, inN-methyl-N'-nitro-N-nitrosoguanidine- induced rat gastric carcinogenesis: immunohistochemical study2011
Author(s)
Motohashi, M., Wakui, S., Muto, T.,Suzuki, Y., Shirai, M., Takahashi, H. and Hano, H
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Journal Title
J. Toxicol. Sci
Volume: 36
Pages: 373-378
Related Report
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[Journal Article] In vitrothermoreversible gel disc quantitative assay of rat angiogenesis2011
Author(s)
Wakui, S., Muto, T., Anzai, N., Takahashi, H., Jutabha, P., Sakata, A., Wempe, M.F.,Hano, H. and Endou, H.
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Journal Title
AATEX
Volume: 16
Pages: 59-65
NAID
Related Report
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[Journal Article] Human uric acid transporter1: an inhibitor structure-activity relationship study2011
Author(s)
Wempe, M.F., Quade, B., Jutabha, P., Iwen, TJ., Frick, M.F., Rice, PJ., Wakui, S.and Endou, H
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Journal Title
Nucleosides Nucleotides Nucleic Acids
Volume: 30
Pages: 1312-1323
Related Report
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[Journal Article] Human drug transporter gene-expressing cells are useful alternatives to predictpharmacokinetics in man2011
Author(s)
Anzai, N., Wakui, S., Jutabha, P., Muto, T., Hayashi, M., Hayashi, K., Domae, M.,Uchida, K., Wempe, M.F. and Endou, H
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Journal Title
AATEX
Volume: 16
Pages: 66-73
NAID
Related Report
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[Journal Article] Apical voltage-driven urate effiluxtransporter NPT4 in renal proximal tubule2011
Author(s)
Jutabha, P., Anzai, N., Wempe, M.F., Wakui, S., Endou, H. and Sakurai, H
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Journal Title
Nucleosides Nucleotides Nucleic Acids
Volume: 30
Pages: 1302-1311
Related Report
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[Journal Article] Potent hURAT1 inhibitors: in vitro and in vivo metabolism and pharmacokineticstudies2011
Author(s)
Wempe, M.F., Rice, P.J., Lightner, J.W., Jutabha, P., Hayashi, M., Anzai, N., Wakui,S., Kusuhara, H., Sugiyama, Y. and Endou,H
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Journal Title
Drug Design, Develop, Therapy
Volume: 30
Pages: 3112-3113
Related Report
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[Journal Article] Testicular spermiation failure in rats exposed prenatally to 3,3',4,4',5- pentachlorobiphenyl2010
Author(s)
Wakui, S., Muto, T., Motohashi, M.,Kobayashi, Y., Suzuki, Y., Shirai, M., Takahashi, H. and Hano, H
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Journal Title
J. Toxicol. Sci
Volume: 35
Pages: 757-765
NAID
Related Report
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[Journal Article] LAT1 antagonists suppressed N-methyl-N'- nitrosoguanidine induced rat gastric carcinogenesis2010
Author(s)
Ishida, K., Muto, T., Motohashi, M.,Kobayashi, Y., Imai, K. and Wakui, S
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Journal Title
J. Toxicol. Pathol
Volume: 23
Related Report
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[Journal Article] Male-female rats gastric carcinogenesis induced by N-methy-N'-nitrosoguanidine phthalate2010
Author(s)
Motohashi, M., Muto, T., Ishida, K.,Kobayashi, Y., Imai, K. and Wakui, S
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Journal Title
J. Toxicol. Pathol
Volume: 23
Related Report
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[Journal Article] Lung and testicular development in ratswith prenatal perfluorooctane-sulfate exposure2010
Author(s)
Kobayashi, Y., Muto, T., Ishida, K., Motohashi, M., Imai, K. and Wakui, S
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Journal Title
J. Toxicol. Pathol.
Volume: 23
Related Report
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[Journal Article] Rats prenatally3,3',4,4',5-Pentachlorobiphenyl (PCB126)Modulate ERK and ERαexpression inN-nitrobis(2-hydroxypropyl)amine (BHP)induced lung carcinogenesis2009
Author(s)
Kobayashi, Y., Ishida, K., Motohashi, M.,Muto, T. and Wakui, S
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Journal Title
J. Toxicol. Pathol
Volume: 22
Related Report
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