Exploration of factors that have an influence on reassortment of influenza A virus
| Project/Area Number |
21580372
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
GOTO Hideo 東京大学, 医科学研究所, 助教 (50323639)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | インフルエンザ / 遺伝子再集合 / 断片化蛋白質 / コンプリメンテーション / 断片化ルシフェラーゼ |
|---|
| Research Abstract |
To explore causes that have an influence on reassortment of influenza A virus, virus like particles that carried HA and NA genome segments expressing split reporter proteins were generated. Reporter activity by protein complementation was detectable in cells transfected with protein expression plasmids. However the activity was reduced to 1/105 of the wild type activity. Due to the significant reduction of the activity the complementation could not be detected upon the VLP infection. The different amount of protein production between HA and NA segments may contribute the undetectable complementation upon VLP infection
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] F1Fo-ATPase, F-type proton-translocating ATPase, at the plasma membrane is critical for efficient influenza virus budding2012
Author(s)
Gorai T, Goto H, Noda T, Watanabe T, Kozuka-Hata H, Oyama M, Takano R, Neumann G, Watanabe S, Kawaoka Y
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Journal Title
Proceedings of National Academy of Sciences of the United States of America
Volume: 109(12)
Issue: 12
Pages: 4615-4620
DOI
Related Report
Peer Reviewed
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[Journal Article] The HA and NS genes of human H5N1 influenza A virus contribute to high virulence in ferrets2010
Author(s)
Imai H, Shinya K, Takano R, Kiso M, Muramoto Y, Sakabe S, Murakami S, Ito M, Yamada S, Le MT, Nidom CA, Sakai-Tagawa Y, Takahashi K, Omori Y, Noda T, Shimojima M, Kakugawa S, Goto H, Iwatsuki-Horimoto K, Horimoto T, Kawaoka Y
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Journal Title
PloS Pathog
Volume: 6
Issue: 9
Pages: e1001106-e1001106
DOI
Related Report
Peer Reviewed
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