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Exploration of factors that have an influence on reassortment of influenza A virus

Research Project

Project/Area Number 21580372
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo

Principal Investigator

GOTO Hideo  東京大学, 医科学研究所, 助教 (50323639)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsインフルエンザ / 遺伝子再集合 / 断片化蛋白質 / コンプリメンテーション / 断片化ルシフェラーゼ
Research Abstract

To explore causes that have an influence on reassortment of influenza A virus, virus like particles that carried HA and NA genome segments expressing split reporter proteins were generated. Reporter activity by protein complementation was detectable in cells transfected with protein expression plasmids. However the activity was reduced to 1/105 of the wild type activity. Due to the significant reduction of the activity the complementation could not be detected upon the VLP infection. The different amount of protein production between HA and NA segments may contribute the undetectable complementation upon VLP infection

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 2009

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (4 results)

  • [Journal Article] F1Fo-ATPase, F-type proton-translocating ATPase, at the plasma membrane is critical for efficient influenza virus budding2012

    • Author(s)
      Gorai T, Goto H, Noda T, Watanabe T, Kozuka-Hata H, Oyama M, Takano R, Neumann G, Watanabe S, Kawaoka Y
    • Journal Title

      Proceedings of National Academy of Sciences of the United States of America

      Volume: 109(12) Issue: 12 Pages: 4615-4620

    • DOI

      10.1073/pnas.1114728109

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reassortment between seasonal H1N1 and pandemic(H1N1) 2009 influenza viruses is restricted by limited compatibility among polymerase subunits2011

    • Author(s)
      Octaviani CP, Goto H, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 85 Issue: 16 Pages: 8449-52

    • DOI

      10.1128/jvi.05054-11

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA2011

    • Author(s)
      Kawakami E, Watanabe T, Fujii K, Goto H, Watanabe S, Noda T, Kawaoka Y
    • Journal Title

      J Virol Methods

      Volume: 173 Issue: 1 Pages: 1-6

    • DOI

      10.1016/j.jviromet.2010.12.014

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The HA and NS genes of human H5N1 influenza A virus contribute to high virulence in ferrets2010

    • Author(s)
      Imai H, Shinya K, Takano R, Kiso M, Muramoto Y, Sakabe S, Murakami S, Ito M, Yamada S, Le MT, Nidom CA, Sakai-Tagawa Y, Takahashi K, Omori Y, Noda T, Shimojima M, Kakugawa S, Goto H, Iwatsuki-Horimoto K, Horimoto T, Kawaoka Y
    • Journal Title

      PloS Pathog

      Volume: 6 Issue: 9 Pages: e1001106-e1001106

    • DOI

      10.1371/journal.ppat.1001106

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] High level of genetic compatibility between swine-origin H1N1 and highly pathogenic avian H5N1 influenza viruses2010

    • Author(s)
      Octaviani CP, Ozawa M, Yamada S, Goto H, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 84 Issue: 20 Pages: 10918-22

    • DOI

      10.1128/jvi.01140-10

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-12010

    • Author(s)
      Arii J, Goto H, Suenaga T, et al. 11名2番目
    • Journal Title

      Nature

      Volume: 167 Issue: 7317 Pages: 859-62

    • DOI

      10.1038/nature09420

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A role for the C terminus of Mopeia virus nucleoprotein in its incorporation into Z protein-induced virus-like particles2010

    • Author(s)
      Shtanko O, Imai M, Goto H, Lukashevich IS, Neumann G, Watanabe T, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 84 Issue: 10 Pages: 5415-22

    • DOI

      10.1128/jvi.02417-09

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The HA and NS genes of human H5N1 influenza A virus contribute to high virulence in ferrets.2010

    • Author(s)
      Imai H
    • Journal Title

      PLoS Pathog.

      Volume: 6

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High level of genetic compatibility between swine-origin H1N1 and highly pathogenicavian H5N1 influenza viruses.2010

    • Author(s)
      Octaviani CP
    • Journal Title

      J Virol.

      Volume: 84 Pages: 10918-10922

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1.2010

    • Author(s)
      Arii J
    • Journal Title

      Nature

      Volume: 467 Pages: 859-862

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA.2010

    • Author(s)
      Kawakami E
    • Journal Title

      J Virol Methods.

      Volume: 173 Pages: 1-6

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A role for the C-terminus of Mopeia virus nucleoprotein in its incorporation into Z-induced virus-like particles.2010

    • Author(s)
      Shtanko O
    • Journal Title

      J Virol. 84

      Pages: 5415-5422

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nucleotide sequence requirements at the 5' end of the influenza A virus M RNA segment for efficient virus replication2009

    • Author(s)
      Ozawa M, Maeda J, Iwatsuki-Horimoto K, Watanabe S, Goto H, Horimoto T, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 83 Issue: 7 Pages: 3384-8

    • DOI

      10.1128/jvi.02513-08

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] TRAF6 establishes innate immune responses by activating NF-kappaB and IRF7 upon sensing cytosolic viral RNA and DNA2009

    • Author(s)
      Konno H, Yamamoto T, Yamazaki K, et al. 16名7番目
    • Journal Title

      PLoS One

      Volume: 4 Issue: 5 Pages: e5674-e5674

    • DOI

      10.1371/journal.pone.0005674

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] RuvB-like protein 2 is a suppressor of influenza A virus polymerases2009

    • Author(s)
      Kakugawa S, Shimojima M, Neumann G, Goto H, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 83 Issue: 13 Pages: 6429-34

    • DOI

      10.1128/jvi.00293-09

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nucleotide sequence requirements at the 5' end of the influenza A virus M RNA segment for efficient virus replication.2009

    • Author(s)
      Ozawa M
    • Journal Title

      J Virol. 83

      Pages: 3384-3388

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TRAF6 establishes innate immune responses by activating NF-kappaB and IRF7 upon sensing cytosolic viral RNA and DNA.2009

    • Author(s)
      Konno H
    • Journal Title

      PLoS One. 4

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RuvB-like protein 2 is a suppressor of influenza A virus polymerases.2009

    • Author(s)
      Kakugawa S
    • Journal Title

      J Virol. 83

      Pages: 6429-6434

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Sumoylation of influenza A virus nonstructural protein 1 is important for type I interferon antagonism and pathogenicity2011

    • Author(s)
      T. Gorai
    • Organizer
      XV International Congress of Virology
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Polymerase subunit compatibility is a key factor in the regulation of reassortment between swine-origin H1N1 and contemporary H5N1 and H1N1 influenza viruses2011

    • Author(s)
      C. P. Octaviani
    • Organizer
      XV International Congress of Virology
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Polymerase subunit compatibility is a key factor in the regulation of reassortment between swine-origin H1N1 and contemporary H5N1 and H1N1 influenza2011

    • Author(s)
      C.P.Octaviani
    • Organizer
      XV International Congress of Virology
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] H5N1インフルエンザウイルス高病原性発揮のメカニズム2010

    • Author(s)
      今井博貴
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      あわぎんホール徳島郷土文化会館(徳島県徳島市)
    • Year and Date
      2010-11-08
    • Related Report
      2011 Final Research Report 2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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