| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Prior study in this familial epileptic Shetland sheepdog has suggested that a decrease of GLT-1 expression in astrocytes in the cortex under the sulcus may be responsible for the occurrence of abnormal electroencephalographic waves. In this study, we investigated where the process of GLT-1 synthesis would be affected in astrocytes. Immunohistochemically, a decrease of GLT-1 immunolabellings was found in the cerebral cortex under the sulcus. In situ hybridization demonstrated no significant changes of GLT-1 mRNA labellings in the cortex under the sulcus of all familial dogs. In immunoelectron microscopy, GLT-1 immunolabellings were detected in endoplasmic reticulum(ER), while not the plasma membrane of astrocytes in the familial cortex under the sulcus. These results suggested at least two possibilities that GLT-1 synthesis process may stop in ER following an incomplete translation, or that the post-translational process of GLT-1 synthesis may be affected.
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